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Graefe's Archive for Clinical and Experimental Ophthalmology

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Open Access 10-06-2022 | Levofloxacin | Cornea

Spectrum and resistance in bacterial infections of the ocular surface in a German tertiary referral center 2009–2019

Authors: Mathias Roth, Paul Goerke, Christoph Holtmann, Andreas Frings, Colin R. MacKenzie, Gerd Geerling

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 12/2022

Abstract

Purpose

Aim of this study was to evaluate the frequencies, trends, and antibiotic resistance of bacteria collected from ocular surface or contact lens material in a German tertiary referral center from 2009 to 2019.

Methods

Microbiological data from 2009 to 2019 was analyzed. Culture-dependent microbial identification and analysis of antibiotic sensitivity was completed by the Institute of Microbiology. Statistical analysis of age- and sex-specific differences as well as changes in the microbial spectrum and resistance over the study period was performed with GraphPad Prism 9.0 applying nonparametric tests (level of significance: p ≦ 0.05).

Results

A total of 6361 specimens were analyzed. Positivity rate was 18.6%. Sixty-three percent (n = 680) of the bacterial isolates were derived from ocular surface and 37% (n = 399) from contact lens material. The ratio of gram-negative bacteria was significantly higher in contact lens material. Multiresistant bacteria showed a significant increase with patient age (p < 0.0001). An overall increase in resistance to levofloxacin (p = 0.0239) was detected. Only 2.4% and 3.1% isolates were resistant to a combination of moxifloxacin and gentamicin, respectively, levofloxacin and gentamicin.

Conclusions

The reported bacterial spectrum is similar to comparable centers. Our data show that it should not be assumed that the newest classes of antibiotics have the best efficacy or lowest resistance levels. In suspected bacterial conjunctivitis, we propose using gentamicin as first-line therapy. In therapy refractive cases and in involvement of the cornea, we recommend a combination of gentamicin and ofloxacin or moxifloxacin. Overall, the evaluated organisms showed good sensitivity to the regularly used antibiotics.

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Title: Spectrum and resistance in bacterial infections of the ocular surface in a German tertiary referral center 2009–2019
Authors: Mathias Roth
Paul Goerke
Christoph Holtmann
Andreas Frings
Colin R. MacKenzie
Gerd Geerling
Publication date: 10-06-2022
Publisher: Springer Berlin Heidelberg
Keywords: Levofloxacin
Conjunctivitis
Moxifloxacin
Gentamicin
Antibiotic
Pseudomonas
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 12/2022
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-022-05721-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Spectrum and resistance in bacterial infections of the ocular surface in a German tertiary referral center 2009–2019

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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