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Open Access 01-12-2016 | Research

Knockdown of ribosomal protein S15A induces human glioblastoma cell apoptosis

Authors: Chen Zhang, Jiqiang Fu, Fei Xue, Bomi Ryu, Ting Zhang, Shuili Zhang, Jingyu Sun, Xinxin Xu, Zhaoli Shen, Longpo Zheng, Xianzhen Chen

Published in: World Journal of Surgical Oncology | Issue 1/2016

Abstract

Background

RPS15A is a ribosome protein that is highly conserved in many organisms from yeast to human. A number of studies implied its role in promoting cancer cell growth.

Methods

Here, we firstly conducted RPS15A gene expression analysis in brain cancer using Oncomine database and found RPS15A was remarkably overexpressed in glioblastoma (GBM) compared with that in normal tissues. Then, the expression of RPS15A was specifically silenced in GBM cell line U251 using lentiviral-mediated RNA interference technique. We further investigated the effect of RPS15A knockdown in U251 cells using MTT assay, colony formation test, and flow cytometry analysis. We detected the protein level of Bcl-2 and poly (ADP-ribose) polymerase (PARP) as well as activation of caspase-3.

Results

Our results showed that the knockdown of RPS15A could inhibit cancer cell growth and colony formation in vitro, as well as induced cell cycle arrest at G0/G1 phase and cell apoptosis. In addition, Western blot analysis indicated that the knockdown of RPS15A could significantly inhibit bcl-2 and activate caspase-3 and PARP.

Conclusions

Our findings suggest RPS15A may play an important role in the progression of GBM and lentiviral-mediated silencing of RPS15A could be an effective tool in GBM treatment.

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Title: Knockdown of ribosomal protein S15A induces human glioblastoma cell apoptosis
Authors: Chen Zhang
Jiqiang Fu
Fei Xue
Bomi Ryu
Ting Zhang
Shuili Zhang
Jingyu Sun
Xinxin Xu
Zhaoli Shen
Longpo Zheng
Xianzhen Chen
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: World Journal of Surgical Oncology / Issue 1/2016
Electronic ISSN: 1477-7819
DOI: https://doi.org/10.1186/s12957-016-0891-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Knockdown of ribosomal protein S15A induces human glioblastoma cell apoptosis

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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