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Cancer Cell International
Published in: Cancer Cell International 1/2021

Open Access 01-12-2021 | Kidney Cancer | Primary research

miR-198 inhibits the progression of renal cell carcinoma by targeting BIRC5

Authors: Chao Yuan, Zhenhong Su, Shengjie Liao, Duanzhuo Li, Zhiwen Zhou, Yawen Wang, Mingchun Quan, Lingling Zeng, Cai Lv, Chenyi Shen, Weida Gong, Jianfeng Wu, Xiaogang Chen, Wenbing Hu, Xu Lv, Wenxia Si, Xin Yu

Published in: Cancer Cell International | Issue 1/2021

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Abstract

Background

miR-198 is involved in the formation, migration, invasion, and metastasis of various malignant cancers. However, the function and mechanism of action of miR-198 in the tumorigenesis of renal cell carcinoma (RCC) remain elusive. Here, we aimed to explore the role of miR198 in RCC.

Methods

Immunohistochemistry was performed to estimate the level of survivin in RCC sections. Quantitative real-time polymerase chain reaction was performed to determine the expression level of miR-198 in fresh RCC tissues. Furthermore, the target relationship between miR-198 and BIRC5 was predicted using the TargetScanHuman 7.2 database and verified via dual-luciferase reporter assay and western blotting. The effects of miR-198 on the viability, apoptosis, invasion, and migration of A498 and ACHN cells were studied using Cell Counting Kit-8, flow cytometry, transwell migration assay, and wound healing assay, respectively. Additionally, a xenograft nude mouse model was established to evaluate the effect of miR-198 on RCC tumorigenesis.

Results

The expression levels of BIRC5 and miR-198 were respectively higher and lower in RCC tissues than those in normal adjacent tissues. Furthermore, miR-198 could inhibit luciferase activity and reduce the protein level of survivin without affecting the BIRC5 mRNA levels. miR-198 inhibited cell viability, migration, and invasion and promoted cell apoptosis; co-transfection with BIRC5 could rescue these effects. Moreover, miR-198 could repress tumor growth in the xenograft nude mouse model of RCC.

Conclusions

Our study demonstrates that miR-198 suppresses RCC progression by targeting BIRC5.
Appendix
Available only for authorised users
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Metadata
Title
miR-198 inhibits the progression of renal cell carcinoma by targeting BIRC5
Authors
Chao Yuan
Zhenhong Su
Shengjie Liao
Duanzhuo Li
Zhiwen Zhou
Yawen Wang
Mingchun Quan
Lingling Zeng
Cai Lv
Chenyi Shen
Weida Gong
Jianfeng Wu
Xiaogang Chen
Wenbing Hu
Xu Lv
Wenxia Si
Xin Yu
Publication date
01-12-2021
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2021
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-021-02092-7

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