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01-02-2013 | Original Paper

Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens

Authors: Byung Ho Kong, Na-Ri Park, Jin-Kyoung Shim, Bo-Kyung Kim, Hye-Jin Shin, Ji-Hyun Lee, Yong-Min Huh, Su-Jae Lee, Se-Hoon Kim, Eui-Hyun Kim, Eun-Kyung Park, Jong Hee Chang, Dong-Seok Kim, Sun Ho Kim, Yong-Kil Hong, Seok-Gu Kang, Frederick F. Lang

Published in: Child's Nervous System | Issue 2/2013

Abstract

Purpose

The existence of cancer stem cells (CSCs) in glioblastoma has been proposed. However, the unknown knowledge that is yet to be revealed is the presence of glioma CSCs (gCSCs) in correlation to each WHO grades of glioma. We approached this study with a hypothesis that specimens from high-grade gliomas would have higher isolation rate of gCSCs in comparison to those of lower-grade gliomas.

Methods

The glioma specimens were obtained from patients and underwent gliomasphere assay. The gliomaspheres were chosen to be analyzed with immunocytochemisty for surface markers. Then the selected gliomaspheres were exposed to neural differentiation conditions. Lastly, we made mouse orthotopic glioma models to examine the capacity of gliomagenesis.

Results

The gliomaspheres were formed in WHO grade IV (13 of 21) and III (two of nine) gliomas. Among them, WHO grade IV (11 of 13) and III (two of two) gliomaspheres showed similar surface markers to gCSCs and were capable of neural differentiation. Lastly, among the chosen cells, 10 of 11 WHO grade IV and two of two WHO grade III gliomaspheres were capable of gliomagenesis. Thus, overall, the rates of existence of gCSCs were more prominent in high-grade gliomas: 47.6 % (10 of 21) in WHO grade IV gliomas and 22.2 % (two of nine) in WHO grade III gliomas, whereas WHO grade II and I gliomas showed virtually no gCSCs.

Conclusions

This trend of stage-by-stage increase of gCSCs in gliomas showed statistical significance by chi-square test linear-by-linear association. We prove that the rates of existence of gCSCs increase proportionally as the WHO grades of gliomas rise.

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Title: Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens
Authors: Byung Ho Kong
Na-Ri Park
Jin-Kyoung Shim
Bo-Kyung Kim
Hye-Jin Shin
Ji-Hyun Lee
Yong-Min Huh
Su-Jae Lee
Se-Hoon Kim
Eui-Hyun Kim
Eun-Kyung Park
Jong Hee Chang
Dong-Seok Kim
Sun Ho Kim
Yong-Kil Hong
Seok-Gu Kang
Frederick F. Lang
Publication date: 01-02-2013
Publisher: Springer-Verlag
Published in: Child's Nervous System / Issue 2/2013
Print ISSN: 0256-7040
Electronic ISSN: 1433-0350
DOI: https://doi.org/10.1007/s00381-012-1964-9

At a glance: The STEP trials

Springer Medicine

Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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