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Archives of Virology
Published in: Archives of Virology 11/2013

01-11-2013 | Original Article

Isolation of Bokeloh bat lyssavirus in Myotis nattereri in France

Authors: Evelyne Picard-Meyer, Alexandre Servat, Emmanuelle Robardet, Marie Moinet, Christophe Borel, Florence Cliquet

Published in: Archives of Virology | Issue 11/2013

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Abstract

Bokeloh bat lyssavirus (BBLV) was found in Myotis nattereri for the first time in northeastern France in July 2012. The complete genome sequence of the virus from the infected Natterer’s bat was determined by whole-genome sequencing and compared to that of the first BBLV strain isolated in 2010 in Germany and with those of all currently identified lyssaviruses. The French isolate [KC169985] showed 98.7 % nucleotide sequence identity to the German BBLV strain [JF311903]. Several organs of the infected French bat were examined by classical rabies diagnostic methods: fluorescent antibody test, cell culture inoculation test and RT-qPCR. Antigen, infectious virus and high viral RNA levels were found in both the brain and salivary glands. Traces of genomic RNA were detected in the bladder, kidney and lung tissue. The results of an investigation of the distribution of lyssaviruses with the detection of infectious virus in the salivary glands suggest a possible mode of transmission of the virus.
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Metadata
Title
Isolation of Bokeloh bat lyssavirus in Myotis nattereri in France
Authors
Evelyne Picard-Meyer
Alexandre Servat
Emmanuelle Robardet
Marie Moinet
Christophe Borel
Florence Cliquet
Publication date
01-11-2013
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 11/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-013-1747-y

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