Published in:
01-01-2008 | Note
Isolation and identification of antioxidants from Pedilanthus tithymaloides
Authors:
Pedro M. Abreu, Susan Matthew, Tania González, Lucie Vanickova, David Costa, Ana Gomes, Marcela A. Segundo, Eduarda Fernandes
Published in:
Journal of Natural Medicines
|
Issue 1/2008
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Abstract
A bioassay-guided methodology utilizing 2,2-diphenyl-1-picrylhydrazyl (DPPH), the trolox equivalent antioxidant capacity (TEAC), and reducing power assays, as well as an assessment of scavenging properties against O
2
·−
, H2O2, HOCl, ROO·, ·NO, and ONOO− were used to find the main antioxidant principles of Pedilanthus tithymaloides (Euphorbiaceae), a shrub used in traditional Cuban medicine. The principles were identified as kaempferol 3-O-β-D-glucopyranoside-6′′-(3-hydroxy-3-methylglutarate), quercitrin, isoquercitrin, and scopoletin. The contents of total phenolics and flavonoids were found to be 76.0 ± 4.8 mg of gallic acid equivalents/g extract and 9.8 ± 0.4 mg of rutin equivalents/g extract, respectively.