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Virology Journal
Published in: Virology Journal 1/2010

Open Access 01-12-2010 | Research

Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan

Authors: Ryo Hoshina, Mayumi Shimizu, Yoichi Makino, Yoshihiro Haruyama, Shin-ichiro Ueda, Yutaka Kato, Masahiro Kasahara, Bun-ichiro Ono, Nobutaka Imamura

Published in: Virology Journal | Issue 1/2010

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Abstract

Background

We performed an environmental study of viruses infecting the symbiotic single-celled algae of Paramecium bursaria (Paramecium bursaria Chlorella virus, PBCV) in Lake Biwa, the largest lake in Japan. The viruses detected were all Chlorella variabilis virus (CvV = NC64A virus). One of them, designated CvV-BW1, was subjected to further characterization.

Results

CvV-BW1 formed small plaques and had a linear DNA genome of 370 kb, as judged by pulsed-field gel electrophoresis. Restriction analysis indicated that CvV-BW1 DNA belongs to group H, one of the most resistant groups among CvV DNAs. Based on a phylogenetic tree constructed using the dnapol gene, CvV was classified into two clades, A and B. CvV-BW1 belonged to clade B, in contrast to all previously identified virus strains of group H that belonged to clade A.

Conclusions

We conclude that CvV-BW1 composes a distinct species within C. variabilis virus.
Appendix
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Metadata
Title
Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan
Authors
Ryo Hoshina
Mayumi Shimizu
Yoichi Makino
Yoshihiro Haruyama
Shin-ichiro Ueda
Yutaka Kato
Masahiro Kasahara
Bun-ichiro Ono
Nobutaka Imamura
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2010
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-7-222

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