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Molecular Imaging and Biology

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01-02-2010 | Research Article

Intravital Imaging of Tumor Apoptosis with FRET Probes During Tumor Therapy

Authors: Feifan Zhou, Da Xing, Shengnan Wu, Wei R. Chen

Published in: Molecular Imaging and Biology | Issue 1/2010

Abstract

Purpose

The aim of the study is to dynamically and non-invasively monitor the apoptosis events in vivo during photodynamic therapy (PDT) and chemotherapy.

Procedures

A FRET probe, SCAT3, was utilized to determine activation of caspase-3 during tumor cell apoptosis in mice, induced by PDT, and cisplatin treatments. Using this method, dynamics of caspase-3 activation was observed both in vitro and in vivo.

Results

Analysis of the fluorescent missions from tumor cells indicated that the caspase-3 activation started immediately after PDT treatment. In contrast, the caspase-3 activation started about 13 and 36 h after cisplatin treatment in vitro and in vivo, respectively.

Conclusions

FRET could be used effectively to monitor activation of caspase-3 in living organism. This method could be used to provide rapid assessment of apoptosis induced by anti-tumor therapies for improvement of treatment efficacy.

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Title: Intravital Imaging of Tumor Apoptosis with FRET Probes During Tumor Therapy
Authors: Feifan Zhou
Da Xing
Shengnan Wu
Wei R. Chen
Publication date: 01-02-2010
Publisher: Springer-Verlag
Published in: Molecular Imaging and Biology / Issue 1/2010
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-009-0235-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Intravital Imaging of Tumor Apoptosis with FRET Probes During Tumor Therapy

Abstract

Purpose

Procedures

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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