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01-02-2010 | Research Article

Bioluminescence Imaging in Mouse Models Quantifies β Cell Mass in the Pancreas and After Islet Transplantation

Authors: John Virostko, Aramandla Radhika, Greg Poffenberger, Zhongyi Chen, Marcela Brissova, Joshua Gilchrist, Brian Coleman, Maureen Gannon, E. Duco Jansen, Alvin C. Powers

Published in: Molecular Imaging and Biology | Issue 1/2010

Abstract

Purpose

We developed a mouse model that enables non-invasive assessment of changes in β cell mass.

Procedures

We generated a transgenic mouse expressing luciferase under control of the mouse insulin I promoter [mouse insulin promoter-luciferase-Vanderbilt University (MIP-Luc-VU)] and characterized this model in mice with increased or decreased β cell mass and after islet transplantation.

Results

Streptozotocin-induced, diabetic MIP-Luc-VU mice had a progressive decline in bioluminescence that correlated with a decrease in β cell mass. MIP-Luc-VU animals fed a high-fat diet displayed a progressive increase in bioluminescence that reflected an increase in β cell mass. MIP-Luc-VU islets transplanted beneath the renal capsule or into the liver emitted bioluminescence proportional to the number of islets transplanted and could be imaged for more than a year.

Conclusions

Bioluminescence in the MIP-Luc-VU mouse model is proportional to β cell mass in the setting of increased and decreased β cell mass and after transplantation.

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Title: Bioluminescence Imaging in Mouse Models Quantifies β Cell Mass in the Pancreas and After Islet Transplantation
Authors: John Virostko
Aramandla Radhika
Greg Poffenberger
Zhongyi Chen
Marcela Brissova
Joshua Gilchrist
Brian Coleman
Maureen Gannon
E. Duco Jansen
Alvin C. Powers
Publication date: 01-02-2010
Publisher: Springer-Verlag
Published in: Molecular Imaging and Biology / Issue 1/2010
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-009-0240-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Bioluminescence Imaging in Mouse Models Quantifies β Cell Mass in the Pancreas and After Islet Transplantation

Abstract

Purpose

Procedures

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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