Top

Published in:

Open Access 01-12-2008 | Research

Interdependency of CEACAM-1, -3, -6, and -8 induced human neutrophil adhesion to endothelial cells

Authors: Keith M Skubitz, Amy PN Skubitz

Published in: Journal of Translational Medicine | Issue 1/2008

Abstract

Members of the carcinoembryonic antigen family (CEACAMs) are widely expressed, and, depending on the tissue, capable of regulating diverse functions including tumor promotion, tumor suppression, angiogenesis, and neutrophil activation. Four members of this family, CEACAM1, CEACAM8, CEACAM6, and CEACAM3 (recognized by CD66a, CD66b, CD66c, and CD66d mAbs, respectively), are expressed on human neutrophils. CD66a, CD66b, CD66c, and CD66d antibodies each increase neutrophil adhesion to human umbilical vein endothelial cell monolayers. This increase in neutrophil adhesion caused by CD66 antibodies is blocked by CD18 mAbs and is associated with upregulation of CD11/CD18 on the neutrophil surface. To examine potential interactions of CEACAMs in neutrophil signaling, the effects on neutrophil adhesion to human umbilical vein endothelial cells of a set of CD66 mAbs was tested following desensitization to stimulation by various combinations of these mAbs. Addition of a CD66 mAb in the absence of calcium results in desensitization of neutrophils to stimulation by that CD66 mAb. The current data show that desensitization of neutrophils to any two CEACAMs results in selective desensitization to those two CEACAMs, while the cells remain responsive to the other two neutrophil CEACAMs. In addition, cells desensitized to CEACAM-3, -6, and -8 were still responsive to stimulation of CEACAM1 by CD66a mAbs. In contrast, desensitization of cells to CEACAM1 and any two of the other CEACAMs left the cells unresponsive to all CD66 mAbs. Cells desensitized to any combination of CEACAMs remained responsive to the unrelated control protein CD63. Thus, while there is significant independence of the four neutrophil CEACAMs in signaling, CEACAM1 appears to play a unique role among the neutrophil CEACAMs. A model in which CEACAMs dimerize to form signaling complexes could accommodate the observations. Similar interactions may occur in other cells expressing CEACAMs.

Available only for authorised users

Khan WN, Frangsmyr L, Teglund S, Israelsson A, Bremer K, Hammarstrom S: Identification of three new genes and estimation of the size of the carcinoembryonic antigen family. Genomics. 1992, 14: 384-390. 10.1016/S0888-7543(05)80230-7.PubMed

Obrink B: CEA adhesion molecules: multifunctional proteins with signal-regulatory properties. Curr Opin Cell Biol. 1997, 9: 616-626. 10.1016/S0955-0674(97)80114-7.PubMed

Skubitz KM, Grunert F, Jantscheff P, Kuroki M, Skubitz AP: Summary of the CD66 Cluster Workshop. Leukocyte Typing VI: White cell differentiation antigens. Edited by: Kishimoto T, Kikutani H, von dem Borne A, Goyert S, Mason D, Misasaka M, Moretta L, Okumura K, Shaw S, Springer T, et al. 1997, New York and London: Garland Publishing, Inc, 922-1000.

Thompson JA, Grunert F, Zimmermann W: Carcinoembryonic antigen gene family: molecular biology and clinical perspectives. J Clin Lab Anal. 1991, 5: 344-366. 10.1002/jcla.1860050510.PubMed

Beauchemin N, Draber P, Dveksler G, Gold P, Gray-Owen S, Grunert F, Hammarstrom S, Holmes KV, Karlsson A, Kuroki M: Redefined nomenclature for members of the carcinoembryonic antigen family. Exp Cell Res. 1999, 252: 243-249. 10.1006/excr.1999.4610.PubMed

Gray-Owen SD, Blumberg RS: CEACAM1: contact-dependent control of immunity. Nat Rev Immunol. 2006, 6: 433-446. 10.1038/nri1864.PubMed

Greicius G, Severinson E, Beauchemin N, Obrink B, Singer BB: CEACAM1 is a potent regulator of B cell receptor complex-induced activation. J Leukoc Biol. 2003, 74: 126-134. 10.1189/jlb.1202594.PubMed

Hammarstrom S: The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin Cancer Biol. 1999, 9: 67-81. 10.1006/scbi.1998.0119.PubMed

Horst AK, Ito WD, Dabelstein J, Schumacher U, Sander H, Turbide C, Brummer J, Meinertz T, Beauchemin N, Wagener C: Carcinoembryonic antigen-related cell adhesion molecule 1 modulates vascular remodeling in vitro and in vivo. J Clin Invest. 2006, 116: 1596-1605. 10.1172/JCI24340.PubMedCentralPubMed

10.

Hsieh JT, Luo W, Song W, Wang Y, Kleinerman DI, Van NT, Lin SH: Tumor suppressive role of an androgen-regulated epithelial cell adhesion molecule (C-CAM) in prostate carcinoma cell revealed by sense and antisense approaches. Cancer Res. 1995, 55: 190-197.PubMed

11.

Ilantzis C, Jothy S, Alpert LC, Draber P, Stanners CP: Cell-surface levels of human carcinoembryonic antigen are inversely correlated with colonocyte differentiation in colon carcinogenesis. Lab Invest. 1997, 76: 703-716.PubMed

12.

Izzi L, Turbide C, Houde C, Kunath T, Beauchemin N: cis-Determinants in the cytoplasmic domain of CEACAM1 responsible for its tumor inhibitory function. Oncogene. 1999, 18: 5563-5572. 10.1038/sj.onc.1202935.PubMed

13.

Kammerer R, Hahn S, Singer BB, Luo JS, von Kleist S: Biliary glycoprotein (CD66a), a cell adhesion molecule of the immunoglobulin superfamily, on human lymphocytes: structure, expression and involvement in T cell activation. Eur J Immunol. 1998, 28: 3664-3674. 10.1002/(SICI)1521-4141(199811)28:11<3664::AID-IMMU3664>3.0.CO;2-D.PubMed

14.

Kammerer R, Stober D, Singer BB, Obrink B, Reimann J: Carcinoembryonic antigen-related cell adhesion molecule 1 on murine dendritic cells is a potent regulator of T cell stimulation. J Immunol. 2001, 166: 6537-6544.PubMed

15.

Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H: Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1. Blood. 2007, 110: 4223-4233. 10.1182/blood-2007-06-097592.PubMed

16.

Kilic N, Oliveira-Ferrer L, Wurmbach JH, Loges S, Chalajour F, Neshat-Vahid S, Weil J, Fernando M, Ergun S: Pro-angiogenic signaling by the endothelial presence of CEACAM1. J Biol Chem. 2005, 280: 2361-2369. 10.1074/jbc.M409407200.PubMed

17.

Klaile E, Muller MM, Kannicht C, Singer BB, Lucka L: CEACAM1 functionally interacts with filamin A and exerts a dual role in the regulation of cell migration. J Cell Sci. 2005, 118: 5513-5524. 10.1242/jcs.02660.PubMed

18.

Kleinerman DI, Dinney CP, Zhang WW, Lin SH, Van NT, Hsieh JT: Suppression of human bladder cancer growth by increased expression of C-CAM1 gene in an orthotopic model. Cancer Res. 1996, 56: 3431-3435.PubMed

19.

Kleinerman DI, Troncoso P, Lin SH, Pisters LL, Sherwood ER, Brooks T, von Eschenbach AC, Hsieh JT: Consistent expression of an epithelial cell adhesion molecule (C-CAM) during human prostate development and loss of expression in prostate cancer: implication as a tumor suppressor. Cancer Res. 1995, 55: 1215-1220.PubMed

20.

Kleinerman DI, Zhang WW, Lin SH, Nguyen TV, von Eschenbach AC, Hsieh JT: Application of a tumor suppressor (C-CAM1)-expressing recombinant adenovirus in androgen-independent human prostate cancer therapy: a preclinical study. Cancer Res. 1995, 55: 2831-2836.PubMed

21.

Krop-Watorek A, Laskowska A, Salwa J, Klopocki AG, Grunert F, Ugorski M: CEA-related proteins on human urothelial cell lines of different transformation grades. Cancer Lett. 1999, 139: 15-22. 10.1016/S0304-3835(98)00363-2.PubMed

22.

Kuespert K, Pils S, Hauck CR: CEACAMs: their role in physiology and pathophysiology. Curr Opin Cell Biol. 2006, 18: 565-571. 10.1016/j.ceb.2006.08.008.PubMed

23.

Kuijpers TW, Hoogerwerf M, Laan van der LJ, Nagel G, Schoot van der CE, Grunert F, Roos D: CD66 nonspecific cross-reacting antigens are involved in neutrophil adherence to cytokine-activated endothelial cells. J Cell Biol. 1992, 118: 457-466. 10.1083/jcb.118.2.457.PubMed

24.

Kuijpers TW, Schoot van der CE, Hoogerwerf M, Roos D: Cross-linking of the carcinoembryonic antigen-like glycoproteins CD66 and CD67 induces neutrophil aggregation. J Immunol. 1993, 151: 4934-4940.PubMed

25.

Kunath T, Ordonez-Garcia C, Turbide C, Beauchemin N: Inhibition of colonic tumor cell growth by biliary glycoprotein. Oncogene. 1995, 11: 2375-2382.PubMed

26.

Lucka L, Budt M, Cichocka I, Danker K, Horstkorte R, Reutter W: C-CAM-mediated adhesion leads to an outside-in dephosphorylation signal. Eur J Biochem. 1999, 262: 541-546. 10.1046/j.1432-1327.1999.00402.x.PubMed

27.

Lund-Johansen F, Olweus J, Symington FW, Arli A, Thompson JS, Vilella R, Skubitz K, Horejsi V: Activation of human monocytes and granulocytes by monoclonal antibodies to glycosylphosphatidylinositol-anchored antigens. Eur J Immunol. 1993, 23: 2782-2791. 10.1002/eji.1830231110.PubMed

28.

Muller MM, Singer BB, Klaile E, Obrink B, Lucka L: Transmembrane CEACAM1 affects integrin-dependent signaling and regulates extracellular matrix protein-specific morphology and migration of endothelial cells. Blood. 2005, 105: 3925-3934. 10.1182/blood-2004-09-3618.PubMed

29.

Nair KS, Zingde SM: Adhesion of neutrophils to fibronectin: role of the cd66 antigens. Cell Immunol. 2001, 208: 96-106. 10.1006/cimm.2001.1772.PubMed

30.

Nollau P, Prall F, Helmchen U, Wagener C, Neumaier M: Dysregulation of carcinoembryonic antigen group members CGM2, CD66a (biliary glycoprotein), and nonspecific cross-reacting antigen in colorectal carcinomas. Comparative analysis by northern blot and in situ hybridization. Am J Pathol. 1997, 151: 521-530.PubMedCentralPubMed

31.

Nollau P, Scheller H, Kona-Horstmann M, Rohde S, Hagenmuller F, Wagener C, Neumaier M: Expression of CD66a (human C-CAM) and other members of the carcinoembryonic antigen gene family of adhesion molecules in human colorectal adenomas. Cancer Res. 1997, 57: 2354-2357.PubMed

32.

Scheffrahn I, Singer BB, Sigmundsson K, Lucka L, Obrink B: Control of density-dependent, cell state-specific signal transduction by the cell adhesion molecule CEACAM1, and its influence on cell cycle regulation. Exp Cell Res. 2005, 307: 427-435. 10.1016/j.yexcr.2005.03.030.PubMed

33.

Screaton RA, Penn LZ, Stanners CP: Carcinoembryonic antigen, a human tumor marker, cooperates with Myc and Bcl-2 in cellular transformation. J Cell Biol. 1997, 137: 939-952. 10.1083/jcb.137.4.939.PubMedCentralPubMed

34.

Singer BB, Klaile E, Scheffrahn I, Muller MM, Kammerer R, Reutter W, Obrink B, Lucka L: CEACAM1 (CD66a) mediates delay of spontaneous and Fas ligand-induced apoptosis in granulocytes. Eur J Immunol. 2005, 35: 1949-1959. 10.1002/eji.200425691.PubMed

35.

Singer BB, Scheffrahn I, Heymann R, Sigmundsson K, Kammerer R, Obrink B: Carcinoembryonic antigen-related cell adhesion molecule 1 expression and signaling in human, mouse, and rat leukocytes: evidence for replacement of the short cytoplasmic domain isoform by glycosylphosphatidylinositol-linked proteins in human leukocytes. J Immunol. 2002, 168: 5139-5146.PubMed

36.

Singer BB, Scheffrahn I, Obrink B: The tumor growth-inhibiting cell adhesion molecule CEACAM1 (C-CAM) is differently expressed in proliferating and quiescent epithelial cells and regulates cell proliferation. Cancer Res. 2000, 60: 1236-1244.PubMed

37.

Skubitz KM, Campbell KD, Skubitz AP: CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils. J Leukoc Biol. 1996, 60: 106-117.PubMed

38.

Stanners CP, DeMarte L, Rojas M, Gold P, Fuks A: Opposite functions for two classes of genes of the human carcinoembryonic antigen family. Tumour Biol. 1995, 16: 23-31.PubMed

39.

Stocks SC, Kerr MA: Stimulation of neutrophil adhesion by antibodies recognizing CD15 (Le(X)) and CD15-expressing carcinoembryonic antigen-related glycoprotein NCA-160. Biochem J. 1992, 288 (Pt 1): 23-27.PubMedCentralPubMed

40.

Stocks SC, Kerr MA, Haslett C, Dransfield I: CD66-dependent neutrophil activation: a possible mechanism for vascular selectin-mediated regulation of neutrophil adhesion. J Leukoc Biol. 1995, 58: 40-48.PubMed

41.

Stocks SC, Ruchaud-Sparagano MH, Kerr MA, Grunert F, Haslett C, Dransfield I: CD66: role in the regulation of neutrophil effector function. Eur J Immunol. 1996, 26: 2924-2932. 10.1002/eji.1830261218.PubMed

42.

Turbide C, Kunath T, Daniels E, Beauchemin N: Optimal ratios of biliary glycoprotein isoforms required for inhibition of colonic tumor cell growth. Cancer Res. 1997, 57: 2781-2788.PubMed

43.

Esteban JM, Felder B, Ahn C, Simpson JF, Battifora H, Shively JE: Prognostic relevance of carcinoembryonic antigen and estrogen receptor status in breast cancer patients. Cancer. 1994, 74: 1575-1583. 10.1002/1097-0142(19940901)74:5<1575::AID-CNCR2820740513>3.0.CO;2-W.PubMed

44.

Jantscheff P, Terracciano L, Lowy A, Glatz-Krieger K, Grunert F, Micheel B, Brummer J, Laffer U, Metzger U, Herrmann R, Rochlitz C: Expression of CEACAM6 in resectable colorectal cancer: a factor of independent prognostic significance. J Clin Oncol. 2003, 21: 3638-3646. 10.1200/JCO.2003.55.135.PubMed

45.

Kim J, Kaye FJ, Henslee JG, Shively JE, Park JG, Lai SL, Linnoila RI, Mulshine JL, Gazdar AF: Expression of carcinoembryonic antigen and related genes in lung and gastrointestinal cancers. Int J Cancer. 1992, 52: 718-725. 10.1002/ijc.2910520509.PubMed

46.

Koops MD, Thompson J, Zimmermann W, Stanners CP: Transcriptional regulation of the non-specific cross-reacting antigen gene, a member of the carcinoembryonic antigen gene family up-regulated in colorectal carcinomas. Eur J Biochem. 1998, 253: 778-786. 10.1046/j.1432-1327.1998.2530778.x.PubMed

47.

Thompson J, Mossinger S, Reichardt V, Engels U, Beauchemin N, Kommoss F, von Kleist S, Zimmermann W: A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members. Int J Cancer. 1993, 55: 311-319. 10.1002/ijc.2910550223.PubMed

48.

Hasegawa K, Pham L, O'Connor MK, Federspiel MJ, Russell SJ, Peng KW: Dual therapy of ovarian cancer using measles viruses expressing carcinoembryonic antigen and sodium iodide symporter. Clin Cancer Res. 2006, 12: 1868-1875. 10.1158/1078-0432.CCR-05-1803.PubMed

49.

Ringhoffer M, Blumstein N, Neumaier B, Glatting G, von Harsdorf S, Buchmann I, Wiesneth M, Kotzerke J, Zenz T, Buck AK: 188Re or 90Y-labelled anti-CD66 antibody as part of a dose-reduced conditioning regimen for patients with acute leukaemia or myelodysplastic syndrome over the age of 55: results of a phase I-II study. Br J Haematol. 2005, 130: 604-613. 10.1111/j.1365-2141.2005.05663.x.PubMed

50.

Zenz T, Glatting G, Schlenk RF, Buchmann I, Dohner H, Reske SN, Bunjes D: Targeted marrow irradiation with radioactively labeled anti-CD66 monoclonal antibody prior to allogeneic stem cell transplantation for patients with leukemia: results of a phase I-II study. Haematologica. 2006, 91: 285-286.PubMed

51.

Barnett TR, Drake L, Pickle W: Human biliary glycoprotein gene: characterization of a family of novel alternatively spliced RNAs and their expressed proteins. Mol Cell Biol. 1993, 13: 1273-1282.PubMedCentralPubMed

52.

Barnett TR, Kretschmer A, Austen DA, Goebel SJ, Hart JT, Elting JJ, Kamarck ME: Carcinoembryonic antigens: alternative splicing accounts for the multiple mRNAs that code for novel members of the carcinoembryonic antigen family. J Cell Biol. 1989, 108: 267-276. 10.1083/jcb.108.2.267.PubMed

53.

Kuroki M, Arakawa F, Matsuo Y, Oikawa S, Nakazato H, Matsuoka Y: Three novel molecular forms of biliary glycoprotein deduced from cDNA clones from a human leukocyte library. Biochem Biophys Res Commun. 1991, 176: 578-585. 10.1016/S0006-291X(05)80223-2.PubMed

54.

Singer BB, Lucka L: CEACAM1. UCSD-Nature Molecule Pages. 2005

55.

Watt SM, Fawcett J, Murdoch SJ, Teixeira AM, Gschmeissner SE, Hajibagheri NM, Simmons DL: CD66 identifies the biliary glycoprotein (BGP) adhesion molecule: cloning, expression, and adhesion functions of the BGPc splice variant. Blood. 1994, 84: 200-210.PubMed

56.

Skubitz KM, Snook RW: Monoclonal antibodies that recognize lacto-N-fucopentaose III (CD15) react with the adhesion-promoting glycoprotein family (LFA-1/HMac-1/gp 150,95) and CR1 on human neutrophils. J Immunol. 1987, 139: 1631-1639.PubMed

57.

Harvath L, Balke JA, Christiansen NP, Russell AA, Skubitz KM: Selected antibodies to leukocyte common antigen (CD45) inhibit human neutrophil chemotaxis. J Immunol. 1991, 146: 949-957.PubMed

58.

Skubitz KM, Campbell KD, Iida J, Skubitz AP: CD63 associates with tyrosine kinase activity and CD11/CD18, and transmits an activation signal in neutrophils. J Immunol. 1996, 157: 3617-3626.PubMed

59.

Parham P: Purification of immunologically active HLA-A and -B antigens by a series of monoclonal antibody columns. J Biol Chem. 1979, 254: 8709-8712.PubMed

60.

Vaporciyan AA, Jones ML, Ward PA: Rapid analysis of leukocyte-endothelial adhesion. J Immunol Methods. 1993, 159: 93-100. 10.1016/0022-1759(93)90145-W.PubMed

61.

Wertheimer SJ, Myers CL, Wallace RW, Parks TP: Intercellular adhesion molecule-1 gene expression in human endothelial cells. Differential regulation by tumor necrosis factor-alpha and phorbol myristate acetate. J Biol Chem. 1992, 267: 12030-12035.PubMed

62.

Benchimol S, Fuks A, Jothy S, Beauchemin N, Shirota K, Stanners CP: Carcinoembryonic antigen, a human tumor marker, functions as an intercellular adhesion molecule. Cell. 1989, 57: 327-334. 10.1016/0092-8674(89)90970-7.PubMed

63.

Eidelman FJ, Fuks A, DeMarte L, Taheri M, Stanners CP: Human carcinoembryonic antigen, an intercellular adhesion molecule, blocks fusion and differentiation of rat myoblasts. J Cell Biol. 1993, 123: 467-475. 10.1083/jcb.123.2.467.PubMed

64.

Jessup JM, Kim JC, Thomas P, Ishii S, Ford R, Shively JE, Durbin H, Stanners CP, Fuks A, Zhou H: Adhesion to carcinoembryonic antigen by human colorectal carcinoma cells involves at least two epitopes. Int J Cancer. 1993, 55: 262-268. 10.1002/ijc.2910550216.PubMed

65.

Oikawa S, Inuzuka C, Kuroki M, Arakawa F, Matsuoka Y, Kosaki G, Nakazato H: A specific heterotypic cell adhesion activity between members of carcinoembryonic antigen family, W272 and NCA, is mediated by N-domains. J Biol Chem. 1991, 266: 7995-8001.PubMed

66.

Oikawa S, Inuzuka C, Kuroki M, Matsuoka Y, Kosaki G, Nakazato H: Cell adhesion activity of non-specific cross-reacting antigen (NCA) and carcinoembryonic antigen (CEA) expressed on CHO cell surface: homophilic and heterophilic adhesion. Biochem Biophys Res Commun. 1989, 164: 39-45. 10.1016/0006-291X(89)91679-3.PubMed

67.

Oikawa S, Kuroki M, Matsuoka Y, Kosaki G, Nakazato H: Homotypic and heterotypic Ca(++)-independent cell adhesion activities of biliary glycoprotein, a member of carcinoembryonic antigen family, expressed on CHO cell surface. Biochem Biophys Res Commun. 1992, 186: 881-887. 10.1016/0006-291X(92)90828-9.PubMed

68.

Olsson H, Wikstrom K, Kjellstrom G, Obrink B: Cell adhesion activity of the short cytoplasmic domain isoform of C-CAM (C-CAM2) in CHO cells. FEBS Lett. 1995, 365: 51-56. 10.1016/0014-5793(95)00436-D.PubMed

69.

Rojas M, DeMarte L, Screaton RA, Stanners CP: Radical differences in functions of closely related members of the human carcinoembryonic antigen gene family. Cell Growth Differ. 1996, 7: 655-662.PubMed

70.

Rojas M, Fuks A, Stanners CP: Biliary glycoprotein, a member of the immunoglobulin supergene family, functions in vitro as a Ca2(+)-dependent intercellular adhesion molecule. Cell Growth Differ. 1990, 1: 527-533.PubMed

71.

Teixeira AM, Fawcett J, Simmons DL, Watt SM: The N-domain of the biliary glycoprotein (BGP) adhesion molecule mediates homotypic binding: domain interactions and epitope analysis of BGPc. Blood. 1994, 84: 211-219.PubMed

72.

Zhou H, Fuks A, Alcaraz G, Bolling TJ, Stanners CP: Homophilic adhesion between Ig superfamily carcinoembryonic antigen molecules involves double reciprocal bonds. J Cell Biol. 1993, 122: 951-960. 10.1083/jcb.122.4.951.PubMed

73.

Brummer J, Neumaier M, Gopfert C, Wagener C: Association of pp60c-src with biliary glycoprotein (CD66a), an adhesion molecule of the carcinoembryonic antigen family downregulated in colorectal carcinomas. Oncogene. 1995, 11: 1649-1655.PubMed

74.

Skubitz KM, Campbell KD, Ahmed K, Skubitz AP: CD66 family members are associated with tyrosine kinase activity in human neutrophils. J Immunol. 1995, 155: 5382-5390.PubMed

75.

Beauchemin N, Kunath T, Robitaille J, Chow B, Turbide C, Daniels E, Veillette A: Association of biliary glycoprotein with protein tyrosine phosphatase SHP-1 in malignant colon epithelial cells. Oncogene. 1997, 14: 783-790. 10.1038/sj.onc.1200888.PubMed

76.

McCaw SE, Schneider J, Liao EH, Zimmermann W, Gray-Owen SD: Immunoreceptor tyrosine-based activation motif phosphorylation during engulfment of Neisseria gonorrhoeae by the neutrophil-restricted CEACAM3 (CD66d) receptor. Mol Microbiol. 2003, 49: 623-637. 10.1046/j.1365-2958.2003.03591.x.PubMed

77.

Iida J, Skubitz AP, McCarthy JB, Skubitz KM: Protein kinase activity is associated with CD63 in melanoma cells. J Transl Med. 2005, 3: 42-10.1186/1479-5876-3-42.PubMedCentralPubMed

78.

McCaw SE, Liao EH, Gray-Owen SD: Engulfment of Neisseria gonorrhoeae: revealing distinct processes of bacterial entry by individual carcinoembryonic antigen-related cellular adhesion molecule family receptors. Infect Immun. 2004, 72: 2742-2752. 10.1128/IAI.72.5.2742-2752.2004.PubMedCentralPubMed

79.

Schmitter T, Pils S, Sakk V, Frank R, Fischer KD, Hauck CR: The granulocyte receptor carcinoembryonic antigen-related cell adhesion molecule 3 (CEACAM3) directly associates with Vav to promote phagocytosis of human pathogens. J Immunol. 2007, 178: 3797-3805.PubMed

80.

Schmitter T, Pils S, Weibel S, Agerer F, Peterson L, Buntru A, Kopp K, Hauck CR: Opa proteins of pathogenic neisseriae initiate Src kinase-dependent or lipid raft-mediated uptake via distinct human carcinoembryonic antigen-related cell adhesion molecule isoforms. Infect Immun. 2007, 75: 4116-4126. 10.1128/IAI.01835-06.PubMedCentralPubMed

81.

Lucka L, Cichocka I, Baumler K, Bechler K, Reutter W: A short isoform of carcinoembryonic-antigen-related rat liver cell-cell adhesion molecule (C-CAM/gp110) mediates intercellular adhesion. Sequencing and recombinant functional analysis. Eur J Biochem. 1995, 234: 527-535. 10.1111/j.1432-1033.1995.527_b.x.PubMed

82.

Pignatelli M, Durbin H, Bodmer WF: Carcinoembryonic antigen functions as an accessory adhesion molecule mediating colon epithelial cell-collagen interactions. Proc Natl Acad Sci USA. 1990, 87: 1541-1545. 10.1073/pnas.87.4.1541.PubMedCentralPubMed

83.

Zhou H, Stanners CP, Fuks A: Specificity of anti-carcinoembryonic antigen monoclonal antibodies and their effects on CEA-mediated adhesion. Cancer Res. 1993, 53: 3817-3822.PubMed

84.

Hunter I, Sawa H, Edlund M, Obrink B: Evidence for regulated dimerization of cell-cell adhesion molecule (C-CAM) in epithelial cells. Biochem J. 1996, 320 (Pt 3): 847-853.PubMedCentralPubMed

85.

Singer BB: Molekulare organization von CD66-molekullen afu neutrophilen granulozyten. Doctoral dissertation. doctoral dissertation. 1996, Albert-Ludwigs-Universitat Freiburg

86.

Bos MP, Grunert F, Belland RJ: Differential recognition of members of the carcinoembryonic antigen family by Opa variants of Neisseria gonorrhoeae. Infect Immun. 1997, 65: 2353-2361.PubMedCentralPubMed

87.

Chen T, Gotschlich EC: CGM1a antigen of neutrophils, a receptor of gonococcal opacity proteins. Proc Natl Acad Sci USA. 1996, 93: 14851-14856. 10.1073/pnas.93.25.14851.PubMedCentralPubMed

88.

Chen T, Grunert F, Medina-Marino A, Gotschlich EC: Several carcinoembryonic antigens (CD66) serve as receptors for gonococcal opacity proteins. J Exp Med. 1997, 185: 1557-1564. 10.1084/jem.185.9.1557.PubMedCentralPubMed

89.

Gray-Owen SD, Dehio C, Haude A, Grunert F, Meyer TF: CD66 carcinoembryonic antigens mediate interactions between Opa-expressing Neisseria gonorrhoeae and human polymorphonuclear phagocytes. Embo J. 1997, 16: 3435-3445. 10.1093/emboj/16.12.3435.PubMedCentralPubMed

90.

Gray-Owen SD, Lorenzen DR, Haude A, Meyer TF, Dehio C: Differential Opa specificities for CD66 receptors influence tissue interactions and cellular response to Neisseria gonorrhoeae. Mol Microbiol. 1997, 26: 971-980. 10.1046/j.1365-2958.1997.6342006.x.PubMed

91.

Hauck CR, Meyer TF, Lang F, Gulbins E: CD66-mediated phagocytosis of Opa52 Neisseria gonorrhoeae requires a Src-like tyrosine kinase- and Rac1-dependent signalling pathway. Embo J. 1998, 17: 443-454. 10.1093/emboj/17.2.443.PubMedCentralPubMed

92.

Leusch HG, Drzeniek Z, Markos-Pusztai Z, Wagener C: Binding of Escherichia coli and Salmonella strains to members of the carcinoembryonic antigen family: differential binding inhibition by aromatic alpha-glycosides of mannose. Infect Immun. 1991, 59: 2051-2057.PubMedCentralPubMed

93.

Sauter SL, Rutherfurd SM, Wagener C, Shively JE, Hefta SA: Binding of nonspecific cross-reacting antigen, a granulocyte membrane glycoprotein, to Escherichia coli expressing type 1 fimbriae. Infect Immun. 1991, 59: 2485-2493.PubMedCentralPubMed

94.

Virji M, Evans D, Hadfield A, Grunert F, Teixeira AM, Watt SM: Critical determinants of host receptor targeting by Neisseria meningitidis and Neisseria gonorrhoeae: identification of Opa adhesiotopes on the N-domain of CD66 molecules. Mol Microbiol. 1999, 34: 538-551. 10.1046/j.1365-2958.1999.01620.x.PubMed

95.

Virji M, Makepeace K, Ferguson DJ, Watt SM: Carcinoembryonic antigens (CD66) on epithelial cells and neutrophils are receptors for Opa proteins of pathogenic neisseriae. Mol Microbiol. 1996, 22: 941-950. 10.1046/j.1365-2958.1996.01551.x.PubMed

96.

Wang J, Gray-Owen SD, Knorre A, Meyer TF, Dehio C: Opa binding to cellular CD66 receptors mediates the transcellular traversal of Neisseria gonorrhoeae across polarized T84 epithelial cell monolayers. Mol Microbiol. 1998, 30: 657-671. 10.1046/j.1365-2958.1998.01102.x.PubMed

97.

Ducker TP, Skubitz KM: Subcellular localization of CD66, CD67, and NCA in human neutrophils. J Leukoc Biol. 1992, 52: 11-16.PubMed

98.

Kuroki M, Matsuo Y, Kinugasa T, Matsuoka Y: Augmented expression and release of nonspecific cross-reacting antigens (NCAs), members of the CEA family, by human neutrophils during cell activation. J Leukoc Biol. 1992, 52: 551-557.PubMed

99.

Tetteroo PA, Bos MJ, Visser FJ, von dem Borne AE: Neutrophil activation detected by monoclonal antibodies. J Immunol. 1986, 136: 3427-3432.PubMed

100.

Ergun S, Kilik N, Ziegeler G, Hansen A, Nollau P, Gotze J, Wurmbach JH, Horst A, Weil J, Fernando M, Wagener C: CEA-related cell adhesion molecule 1: a potent angiogenic factor and a major effector of vascular endothelial growth factor. Mol Cell. 2000, 5: 311-320. 10.1016/S1097-2765(00)80426-8.PubMed

101.

Brummer J, Ebrahimnejad A, Flayeh R, Schumacher U, Loning T, Bamberger AM, Wagener C: cis Interaction of the cell adhesion molecule CEACAM1 with integrin beta(3). Am J Pathol. 2001, 159: 537-546.PubMedCentralPubMed

102.

Dveksler G, Nedellec P, Lu JH, Keck U, Basile A, Cardellichio C, Zimmermann W, Beauchemin N, Holmes KV: Characterization of a new gene that encodes a functional MHV receptor and progress in the identification of the virus-binding site(s). Adv Exp Med Biol. 1995, 380: 345-350.PubMed

103.

Dveksler GS, Dieffenbach CW, Cardellichio CB, McCuaig K, Pensiero MN, Jiang GS, Beauchemin N, Holmes KV: Several members of the mouse carcinoembryonic antigen-related glycoprotein family are functional receptors for the coronavirus mouse hepatitis virus-A59. J Virol. 1993, 67: 1-8.PubMedCentralPubMed

104.

Dveksler GS, Pensiero MN, Cardellichio CB, Williams RK, Jiang GS, Holmes KV, Dieffenbach CW: Cloning of the mouse hepatitis virus (MHV) receptor: expression in human and hamster cell lines confers susceptibility to MHV. J Virol. 1991, 65: 6881-6891.PubMedCentralPubMed

105.

Holmes KV, Dveksler G, Gagneten S, Yeager C, Lin SH, Beauchemin N, Look AT, Ashmun R, Dieffenbach C: Coronavirus receptor specificity. Adv Exp Med Biol. 1993, 342: 261-266.PubMed

106.

Holmes KV, Tresnan DB, Zelus BD: Virus-receptor interactions in the enteric tract. Virus-receptor interactions. Adv Exp Med Biol. 1997, 412: 125-133.PubMed

107.

Kammerer R, von Kleist S: The carcinoembryonic antigen (CEA) modulates effector-target cell interaction by binding to activated lymphocytes. Int J Cancer. 1996, 68: 457-463. 10.1002/(SICI)1097-0215(19961115)68:4<457::AID-IJC10>3.0.CO;2-2.PubMed

108.

Morales VM, Christ A, Watt SM, Kim HS, Johnson KW, Utku N, Texieira AM, Mizoguchi A, Mizoguchi E, Russell GJ: Regulation of human intestinal intraepithelial lymphocyte cytolytic function by biliary glycoprotein (CD66a). J Immunol. 1999, 163: 1363-1370.PubMed

Title: Interdependency of CEACAM-1, -3, -6, and -8 induced human neutrophil adhesion to endothelial cells
Authors: Keith M Skubitz
Amy PN Skubitz
Publication date: 01-12-2008
Publisher: BioMed Central
Published in: Journal of Translational Medicine / Issue 1/2008
Electronic ISSN: 1479-5876
DOI: https://doi.org/10.1186/1479-5876-6-78

Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin

Prof. Florian Limbourg

Prof. Anoop Chauhan

Developed by: Springer Medicine

Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits

Illustration of figure on mountain summit/© Orapun / Stock.adobe.com, STEP AAG HeroTeaserCollection image/© Tarek / Generated with AI / Stock.adobe.com, ACC 2024 Pediatric and Congenital Heart Disease: Year in Review/© 2024 American College of Cardiology, ACC 2024 Pulmonary Vascular Disease: Year in Review/© 2024 American College of Cardiology, ACC 2024 Valvular Heart Disease: Year in Review/© 2024 American College of Cardiology, ACC 2024 HF and Cardiomyopathies: Year in Review/© 2024 American College of Cardiology, Woman out walking/© Seventyfour / stock.adobe.com (symbolic image with model), Woman checking smartwatch /© saltdium / stock.adobe.com (symbolic image with model), Cardiac catheterization/© Damian / stock.adobe.com

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 1/2008

Multilineage hematopoietic recovery with concomitant antitumor effects using low dose Interleukin-12 in myelosuppressed tumor-bearing mice

Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients

Comparison between conventional and "clinical" assessment of experimental lung fibrosis

Pathways to ischemic neuronal cell death: are sex differences relevant?

Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor antagonist

Gene expression profiling for molecular distinction and characterization of laser captured primary lung cancers

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

Highlights from the ACC 2024 Congress

ACC 2024 Congress Coverage