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Arthritis Research & Therapy

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Open Access 01-12-2012 | Research article

Intensity-dependent effect of treadmill running on lubricin metabolism of rat articular cartilage

Authors: Guo-Xin Ni, Lei Lei, Yue-Zhu Zhou

Published in: Arthritis Research & Therapy | Issue 6/2012

Abstract

Introduction

We aimed to understand the changes in cartilage lubricin expression and immunolocalisation in responsed to treadmill running with different intensities in a rat model.

Methods

A total of 24 male Wistar rats were randomly assigned into groups of control (CON), low-intensity running (LIR), moderate-intensity running (MIR), and high-intensity running (HIR). Rats in LIR, MIR, and HIR groups were trained for 8 weeks on the treadmill with low, moderate, and high intensity, respectively. After sacrifice, femoral condyles were collected to take histological observation for cartilage characteristics, and immunohistochemistry for lubricin. In addition, cartilage samples were obtained to assess PRG4 and TGF-β mRNA expression by quantitative RT-PCR.

Results

Histological examination showed osteoarthritic changes in rats after eight weeks of high intensity running. In comparison to CON group, significantly lower Mankin score was found in LIR and MIR groups, whereas, HIR group had significantly higher Mankin score than either CON, LIR, or MIR group. On the other hand, both LIR and MIR groups have significantly higher lubricin content than CON group, whereas, significantly lower lubricin content was found in HIR group compared with CON, LIR or MIR group. A significant inverse correlation was detected between the lubricin content and Mankin score. In addition, considerably higher mRNA gene expression of PRG4 and TGF-β was found in LIR and MIR groups, compared with those in CON and HIR groups.

Conclusions

There is a marked intensity-specific effect of running on the immunolocalisation and gene expression of lubricin in cartilage, which is inversely correlated with Mankin score. Our findings provide evidences that mechanical factors are key determinants of lubricin metabolism in vivo.

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Title: Intensity-dependent effect of treadmill running on lubricin metabolism of rat articular cartilage
Authors: Guo-Xin Ni
Lei Lei
Yue-Zhu Zhou
Publication date: 01-12-2012
Publisher: BioMed Central
Published in: Arthritis Research & Therapy / Issue 6/2012
Electronic ISSN: 1478-6362
DOI: https://doi.org/10.1186/ar4101

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Abstract

Introduction

Methods

Results

Conclusions

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