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Open Access 01-12-2022 | Research

Integrated strategy of network analysis prediction and experimental validation to elucidate the possible mechanism of compound Turkish gall ointment in treating eczema

Authors: Xuan Ma, Meng Hao, Ming Hui Zhang, Ya Zeng, Qing Qing Yang, Lu Zhao, Chen Yang Fan, Zhi Hong Ji, Ke Ao Li, Zhi Jian Li, Mirzat Maimaiti, Ji Hong Nie

Published in: Chinese Medicine | Issue 1/2022

Abstract

Background

Compound Turkish gall ointment (CTGO) has a long history of being widely used as a folk medicine in Xinjiang for the treatment of eczema. CTGO is currently in the pre-investigational new drug application stage, but its pharmacological mechanisms of action have not yet been clarified.

Methods

First, a sensitive and reliable ultra-high performance liquid chromatography-Q exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry (UHPLC-Q-Orbitrap HRMS) technique was established. Second, an integrative strategy of network analysis and molecular docking based on identified and retrieved ingredients was implemented to investigate the potential targets and pathways involved in the treatment of eczema with CTGO. Finally, Sprague–Dawley (SD) rats with eczema were prepared to verify the predicted results. The skin conditions of the rats were observed, evaluated, and scored. Skin tissues were observed by hematoxylin–eosin (HE) staining, and the levels of serum interferon-γ (IFN-γ) and interleukin-4 (IL-4) were determined by enzyme-linked immunosorbent assay (ELISA). The expression levels of toll-like receptor 4 (TLR4), nuclear factor kappa-B p65 (NF-κB p65), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were detected by real-time quantitative polymerase chain reaction (RT-qPCR).

Results

A total of 29 compounds were identified. We found 38 active components and 58 targets for the treatment of eczema, which included 118 signaling pathways related to inflammation, immunity, and apoptosis. CTGO significantly improved the skin surface and histopathological characteristics of eczema-affected rats, downregulated the expression of IL-4, TLR4, NF-κB (p65), IL-1β, and TNF-α, and upregulated the expression level of IFN-γ.

Conclusion

We predicted and validated our prediction that CTGO may be used to treat eczema by affecting the TLR4/NF-κB signaling pathway, which provides guidance for future experimental studies.

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Title: Integrated strategy of network analysis prediction and experimental validation to elucidate the possible mechanism of compound Turkish gall ointment in treating eczema
Authors: Xuan Ma
Meng Hao
Ming Hui Zhang
Ya Zeng
Qing Qing Yang
Lu Zhao
Chen Yang Fan
Zhi Hong Ji
Ke Ao Li
Zhi Jian Li
Mirzat Maimaiti
Ji Hong Nie
Publication date: 01-12-2022
Publisher: BioMed Central
Published in: Chinese Medicine / Issue 1/2022
Electronic ISSN: 1749-8546
DOI: https://doi.org/10.1186/s13020-022-00643-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Integrated strategy of network analysis prediction and experimental validation to elucidate the possible mechanism of compound Turkish gall ointment in treating eczema

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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