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Archives of Virology
Published in: Archives of Virology 7/2012

Open Access 01-07-2012 | Brief Report

Inhibition of HSV-1 by chemoattracted neutrophils: supernatants of corneal epithelial cells (HCE) and macrophages (THP-1) treated with virus components chemoattract neutrophils (PMN), and supernatants of PMN treated with these conditioned media inhibit viral growth

Authors: Kozaburo Hayashi, Laura C. Hooper, Toshiomi Okuno, Yuichiro Takada, John J. Hooks

Published in: Archives of Virology | Issue 7/2012

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Abstract

The role of PMNs (neutrophils) in corneal herpes was studied using an in vitro system. Human corneal cells (HCE) and macrophages (THP-1) infected with HSV-1 or treated with virus components (DNA or virus immune complexes) released chemokines, which attracted PMNs. Highly reactive oxygen species were detected in PMNs. PMNs inhibited HSV when overlaid onto infected HCE cells (50:1). PMNs incubated with the supernatants of HCE cells treated with virus components released H2O2 and myeloperoxidase. These inhibited virus growth. PMNs released NO and MIG, which may differentiate CD4 T cells to Th1. PMNs participate in innate immune responses, limit virus growth, and initiate immunopathology.
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Metadata
Title
Inhibition of HSV-1 by chemoattracted neutrophils: supernatants of corneal epithelial cells (HCE) and macrophages (THP-1) treated with virus components chemoattract neutrophils (PMN), and supernatants of PMN treated with these conditioned media inhibit viral growth
Authors
Kozaburo Hayashi
Laura C. Hooper
Toshiomi Okuno
Yuichiro Takada
John J. Hooks
Publication date
01-07-2012
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 7/2012
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1306-y

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