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Clinical Oral Investigations
Published in: Clinical Oral Investigations 9/2015

01-12-2015 | Original Article

Inhibition of adherence of C. albicans to dental implants and cover screws by Cymbopogon nardus essential oil and citronellal

Authors: Leonardo Antunes Trindade, Julyana de Araújo Oliveira, Ricardo Dias de Castro, Edeltrudes de Oliveira Lima

Published in: Clinical Oral Investigations | Issue 9/2015

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Abstract

Objective

This study investigated the biological activity of the essential oil from Cymbopogon nardus and of the phytoconstituent citronellal on Candida strains as to the inhibition of adherence to dental implants and cover screws.

Material and methods

The essential oil was analyzed by gas chromatography coupled to mass spectrometry (GC-MS) and had its MIC and MFC determined against 12 strains of Candida. Then, tests of inhibition of adherence to the dental implants and cover screws were carried out using the MIC of the substances, followed by scanning electron microscopy analysis. Nystatin and chlorhexidine were used as positive controls, and experiments were performed in triplicate.

Results

The analysis by GC-MS of the essential oil identified citronellal as the major compound. The MICs of the essential oil, citronellal, chlorhexidine, and nystatin—able to inhibit 100 % of the strains—were found to be 64, 512, 64, and 32 μg/ml, respectively. The essential oil significantly inhibited the adherence of Candida albicans to the dental implants and cover screws (p < 0.001). Citronellal inhibited yeast adherence only to the dental implants (p < 0.001), and no significant results were found for the cover screws (p > 0.05) compared to the growth control.

Conclusion

The essential oil and citronellal have proven antifungal activity and are able to inhibit the in vitro adherence of C. albicans.
Clinical relevance: There has been a search for alternative natural product-containing formulations that should be effective in inhibiting adherence of yeasts to the surfaces of materials and also able to treat oral fungal infections. Further trials could make these products an alternative to chemical removal of peri-implant biofilm.
Literature
1.
Roos-Jansaker AM, Lindahl C, Renvert H, Renvert S (2006) Nine-to fourteen-year follow-up of implant treatment. Part II: presence of peri-implant lesions. J Clin Periodontol 33:290–295CrossRefPubMed
2.
Schwartz-Arad D, Samet N, Samet N, Mamlider A (2002) Smoking and complications of endosseous dental implants. J Periodontol 73(2):153–157CrossRefPubMed
3.
Van Steenberghe D, Jacobs R, Desnyder M, Maffei G, Quirynen M (2002) The relative impact of local and endogenous patient-related. Clin Oral Implants Res 13(6):617–622CrossRefPubMed
4.
Heitz-Mayfield LJA (2008) Peri-implant diseases: diagnosis and risk indicators. J Clin Periodontol 35(suppl 8):293–304
5.
Lindhe J, Meyle J (2008) Peri-implant diseases: consensus report of the sixth European workshop on periodontology. J Clin Periodontol 35(suppl8):282–285CrossRefPubMed
6.
7.
8.
Lang NP, Wilson TG, Corbet EF (2000) Biological complications with dental implants: their prevention, diagnosis and treatment. Clin Oral lmplants Res 11(suppl 1):146–155CrossRef
9.
Pye AD, Lockhart DEA, Dawson MP, Murray CA, Smith AJ (2009) A review of dental implants and infection. J Hosp Infect 72(2):104–110CrossRefPubMed
10.
Burgers R, Hahnel S, Reichert TE, Rosentritt M, Behr M, Gerlach T, Handel G, Gosau M (2010) Adhesion of Candida albicans to various dental implant surfaces and the influence of salivary pellicle proteins. Acta Biomater 6:2307–2313CrossRefPubMed
11.
Villard N, Seneviratne C, Tsoi JKH, Heinonen M, Matinlinna J (2015) Candida albicans aspects of novel silane system-coated titanium and zirconia implant surfaces. Clin Oral Implants Res 26(3):332–341
12.
Ramage G, Martinez JP, Lopez-Ribot JL (2006) Candida biofilms on implanted biomaterials: a clinically significant problem. FEMS Yeast Res 6:979–986CrossRefPubMed
13.
Tsang CSP, Ng H, Mc Millan AS (2007) Antifungal susceptibility of Candida albicans biofilms on titanium discs with different surface roughness. Clin Oral Invest 11:361–368CrossRef
14.
Leonhardt A, Bergstrom C, Lekholm U (2003) Microbiologic diagnostics at titanium implants. Clin Implant Dent Relat Res 5:226–232CrossRefPubMed
15.
Monge RA, Roma’n E, Nombela C, Pla J (2006) The MAP Kinase signal transduction network in Candida albicans. Microbiology 152(1):905–912CrossRefPubMed
16.
Blankenship JR, Mitchell AP (2006) How to build a biofilm: a fungal perspective. Curr Opin Microbiol 9:588–594CrossRefPubMed
17.
Triplett RG, Andrews JA, Hallmon WW (2003) Management of peri-implantitis. Oral Maxillofac Surg Clin North Am 15(1):129–138CrossRefPubMed
18.
Menendez A, Li F, Michalek SM, Kirk K, Makhija SK, Childers NK (2005) Comparative analysis of the antibacterial effects of combined mouthrinses on Streptococcus mutans. Oral Microbiol Immunol 20:31–34CrossRefPubMed
19.
Renvert S, Roos-Jansaker AM, Claffey N (2008) Non-surgical treatment of peri-implant mucositis and peri-implantitis: a literature review. J Clin Periodontol 35:305–315CrossRefPubMed
20.
Heitz-Mayfield LJ, Salvi GE, Mombelli A, Faddy M, Lang NP (2012) Anti-infective surgical therapy of peri-implantitis. A 12-month prospective clinical study. Clin Oral Implants Res 23:205–210CrossRefPubMed
21.
Wiltfang J, Zernial O, Behrens E, Schlegel A, Warnke PH, Becker ST (2012) Regenerative treatment of peri-implantitis bone defects with a combination of autologous bone and a demineralized xenogenic bone graft: a series of 36 defects. Clin Implant Dent Relat Res 14:421–427CrossRefPubMed
22.
Paraskevas S (2005) Randomized controlled clinical trials on agents used for chemical plaque control. Int J Dent Hyg 3(4):162–178CrossRefPubMed
23.
Gunsolley JC (2006) A meta-analysis of six-month studies of antiplaque and antigingivitis agents. J Am Dent Assoc 137:1649–1657CrossRefPubMed
24.
Bansod S, Rai M (2008) Antifungal activity of essential oils from Indian medicinal plants against human pathogenic Aspergillus fumigates and A. niger. World J Med Sci 3(2):81–88
25.
Calixto JB (2005) Twenty-five years of research on medicinal plants in Latin America: a personal review. J Ethnopharmacol 100:131–134CrossRefPubMed
26.
Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils—a review. Food Chem Toxicol 46:446–475CrossRefPubMed
27.
Cleeland R, Squires E (1991) Evaluation of new antimicrobials in vitro and in experimental animal infections In: Lorian VAntibiotics in laboratory medicine. 3rd edn. Baltimore: Williams and Wilkiam, pp 739–787
28.
Hadacek F, Greger H (2000) Testing of antifungal natural products: methodologies, comparability of results and assay choice. Phytochem Anal 11:137–147CrossRef
29.
Bauer AWMM, Kirby JC, Turck M (1966) Antibiotic susceptibility testing by astandardized single disk method. Am J Clin Pathol 45(3):493–496PubMed
30.
Deswal DP, Chand U (1997) Standartization of the tetrazolium test for viability estimation in ricebean (Vigna umbellate T.) seeds. Seed Sci Technol 25:409–417
31.
Ernst ME, Klepser ME, Wolfe EJ, Pfaller MA (1996) Antifungal Dynamics of LY 303366, an investigational echinocandin B analog, against Candida spp. Diagn Micr Infec Dis 26:125–131CrossRef
32.
Carreto CFP, Navas EAFA, Paradella TC, Oliveira LD, Junqueira JC, Jorge AOC (2007) Efeitos do chá de tomilho sobre a aderência in vitro de Streptococcusmutansao esmalte dentário e Candida albicansà resina acrílica. Rev Odontol UNESP 36(3):281–186
33.
Billerbeck VG, Roques CG, Bessière JM, Fonvielle JL, Dargent R (2001) Effects of Cymbopogonnardus (L.) W. Watson essential oil on the growth and morphogenesis of Aspergillusniger. Can J Microbiol 47(1):9–17CrossRefPubMed
34.
Zore GB, Thakre AD, Jadhav S, Karuppayil SM (2011) Terpenoids inhibit Candida albicans growth by affecting membrane integrity and arrest of cell cycle. Phytomedicine 18:1181–1190CrossRefPubMed
35.
Koba K, Sanda K, Guyon C, Raynaud C, Chaumont JP, Nicod L (2009) In vitro cytotoxic activity of CymbopogoncitratusL. and CymbopogonnardusL. essential oils from Togo. Bangladesh J Pharmacol 4:29–34
36.
Kpoviessi S, Bero J, Agbani P, Gbaguidi F, Kpadonou-Kpoviessi B, Sinsin B, Accrombessi G, FrédérichM MM, Quetin-Leclercq J (2014) Chemical composition, cytotoxicity and in vitroantitrypanosomal and antiplasmodial activity of the essential oils of four Cymbopogonspecies from Benin. J Ethnopharmacol 151:652–659CrossRefPubMed
37.
Wei LS, Wee W (2013) Chemical composition and antimicrobial activity of Cymbopogonnarduscitronella essential oil against systemic bacteria of aquatic animals. Iran J Microbiol 5(2):147–152PubMedCentralPubMed
38.
Roszaini K, NorAzah MA, Mailina J, Zaini S, Faridz ZM (2013) Toxicity and antitermite activity of the essential oils from Cinnamomumcamphora, Cymbopogonnardus, Melaleucacajuputiand Dipterocarpussp. against Coptotermescurvignathus. Wood Sci Technol 47:1273–1284CrossRef
39.
Ozcan M, Erkmen O (2001) Antimicrobial activity of the essential oils of Turkish plant spices. Eur Food Res Technol 212(6):658–660CrossRef
40.
Williamson EM (2001) Synergy and other interactions in phytomedicines. Phytomedicine 8(5):401–409CrossRefPubMed
41.
Li WR, Shi QS, Ouyang YS, Chen YB, Duan SS (2013) Antifungal effects of citronela oil against Aspergillusniger ATCC 16404. Appl Microbiol Biotechnol 97(16):7483–7492CrossRefPubMed
42.
Wongsariya K, Phanthong P, Bunyapraphatsara N, Srisukh V, Chomnawang MT (2014) Synergistic interaction and mode of action of Citrus hystrix essential oil against bacterial causing periodontal diseases. Pharm Biol 52(3):273–280CrossRefPubMed
43.
Corrêa NB, Gonçalves GMS (2010) Atividade antifúngica de óleos essenciais de plantas aromáticas frente a diferentes espécies de Candida. Anais do XV encontro de iniciação científica da PUC-Campinas
44.
Chandra J, Mukherjee PK, Leidich SD, Faddoul FF, Hoyer LL, Douglas LJ, Ghannoum MA (2001) Antifungal resistance of candidal biofilms formed on denture acrylic in vitro. J Dent Res 80:903–908CrossRefPubMed
45.
Kuhn DM, George T, Chandra J, Mukherjee PK, Ghannoum MA (2002) Antifungal susceptibility of Candida biofilms: unique efficacy of amphotericin B lipid formulations and echinocandins. Antimicrob Agents Chemother 46:1773–1780PubMedCentralCrossRefPubMed
46.
47.
Cox SD, Mann CM, Markham JL, Bell HC, Gustafson JE, Warmington JR, Wyllic SG (2000) The mode of antimicrobial action of essential oils of Melaleucaalternifolia(tea tree oil). J Appl Microbiol 88:170–175CrossRefPubMed
48.
Harder S, Podschun R, Grancicova L, Mehl C, Kern M (2013) Analysis of the intraimplantmicroflora of two-piece dental implants. Clin Oral Invest 17:1135–1142CrossRef
49.
Shibli JA, Martins MC, Lotufo RFM, Marcantonio E (2003) Microbiologic and radiographic analysis of ligature-induced peri-implantitis with diferente dental implant surfaces. Int J Oral Maxillofac Implants 18(3):383–390PubMed
50.
Nasatzky E, Gultchin J, Schwartz Z (2003) The role of surface roughness in promoting osteointegration. Refuat Hapeh Vehashinayim 20(3):8–19
51.
Wennerberg A, Albrektsson T (2000) Suggested guidelines for the topographic evaluation of implant surfaces. Int J Oral Maxillofac Implants 15:331–344PubMed
52.
Novaes AB Jr, Papalexiou V, Grisi MF, Souza SS, Taba M Jr, Kajiwara JK (2004) Influence of implant microstructure on the osseointegration of immediate implants placed in periodontally infected sites. A histomorphometric study in dogs. Clin Oral Implants Res 15:34–43CrossRefPubMed
53.
Mimica LMJ, Ueda SMY, Martino MDV, Navarini A, Martini IJ (2009) Candida infection diagnosis: evaluation of Candida species identification and characterization of susceptibility profile. J Bras Pat Med Lab 5(1):17–23
54.
Quirynen M, Van Der Mei HC, Bollen CM, Schotte A, Marechal M, Doornbusch GI, NAERT I, Busscher HJ, Van Steenberghe D (1993) An in vivo study of the influence of the surface roughness of implants on the microbiology of supra- and subgingival plaque. J Dent Res 72(9):1304–1309CrossRefPubMed
Metadata
Title
Inhibition of adherence of C. albicans to dental implants and cover screws by Cymbopogon nardus essential oil and citronellal
Authors
Leonardo Antunes Trindade
Julyana de Araújo Oliveira
Ricardo Dias de Castro
Edeltrudes de Oliveira Lima
Publication date
01-12-2015
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 9/2015
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-015-1450-3

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