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Open Access 01-12-2015 | Original Article

Efficacy of cleansing agents in killing microorganisms in mixed species biofilms present on silicone facial prostheses—an in vitro study

Authors: Nina Ariani, Anita Visser, Margot R. I. M. Teulings, Melissa Dijk, Tri Budi W. Rahardjo, Arjan Vissink, Henny C. van der Mei

Published in: Clinical Oral Investigations | Issue 9/2015

Abstract

Objectives

The purpose of this study was to assess the efficacy of different cleansing agents in killing mixed species biofilms on silicone facial prostheses.

Materials and methods

Two bacterial and three yeast strains, isolated from silicone facial prostheses, were selected for the mixed species biofilms. A variety of agents used to clean facial prostheses were employed, viz., antibacterial soap, essential-oil-containing mouth rinse, ethanol 27 %, chlorhexidine mouth rinse, and buttermilk. Colony forming units (CFUs) and live/dead staining were analyzed to assess the efficacy of these cleansing agents against 24-h and 2-week biofilms and regrown biofilms on silicone samples.

Results

Chlorhexidine was the most effective cleansing agent. Chlorhexidine killed 8 log unit CFUs (>99.99 % killing) in a 24-h biofilm and 5 log unit CFUs (>99.99 % killing) in 2-week biofilms. Also, after regrowth and repeated treatment of the biofilm, chlorhexidine was the most effective cleansing agent showing no detectable CFUs. The essential-oil-containing mouth rinse (containing 26.9 % ethanol) showed a similar efficacy as ethanol (27 %) alone. Antibacterial soap and buttermilk were the least effective agents tested.

Conclusions

Chlorhexidine showed the highest reduction in CFUs in 24-h, 2-week, and regrown mixed species biofilm of microorganisms isolated from silicone facial prostheses.

Clinical relevance

Chlorhexidine mouth rinse (easy obtainable and relatively cheap) is very effective in killing bacteria and yeast present in biofilms on silicone facial prostheses. When applied on a regular basis, cleansing a facial prosthesis with chlorhexidine will presumably increase its lifetime and reduce skin irritations.

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Title: Efficacy of cleansing agents in killing microorganisms in mixed species biofilms present on silicone facial prostheses—an in vitro study
Authors: Nina Ariani
Anita Visser
Margot R. I. M. Teulings
Melissa Dijk
Tri Budi W. Rahardjo
Arjan Vissink
Henny C. van der Mei
Publication date: 01-12-2015
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 9/2015
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-015-1453-0

At a glance: The STEP trials

Springer Medicine

Efficacy of cleansing agents in killing microorganisms in mixed species biofilms present on silicone facial prostheses—an in vitro study

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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