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European Journal of Clinical Microbiology & Infectious Diseases

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01-01-2021 | Influenza Virus | Original Article

miR-1975 serves as an indicator of clinical severity upon influenza infection

Authors: Yuag-Meng Liu, Hui-Chen Chen, Yi-Chun Chen, Wen-Ya Yu, Meng-Yen Ho, Chia-Yin Ho, Michael M.C. Lai, Wen-Chi Su

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 1/2021

Abstract

Emerging evidence highlights the role of non-coding small RNAs in host-influenza interaction. We have identified a Y RNA-derived small RNA, miR-1975, which is upregulated upon influenza A virus infection in A549 cells. The aim of this study is to investigate whether miR-1975 serves as an indicator of clinical severity upon influenza infection. We investigate the abundance of miR-1975 in sera from clinical patients and its correlation with hypoxemia status. We quantified its amounts in sera from influenza virus-infected patients and healthy volunteers by means of stem-loop RT-PCR. Median values of miR-1975 were significantly higher in influenza virus-infected patients, especially in hypoxemic patients. miR-1975 levels at the acute stage of the disease were highly correlated with the fraction of inspired oxygen used by the patients and total ventilator days. Receiver operator characteristic curve analysis revealed that miR-1975 levels in combination with days of fever before presenting to hospital had significant predictive value for hypoxemia and respiratory failure for patients infected with influenza virus. Our results reveal that circulating miR-1975 has great potential to serve as a biomarker for predicting prognosis in patients infected with influenza virus.

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Title: miR-1975 serves as an indicator of clinical severity upon influenza infection
Authors: Yuag-Meng Liu
Hui-Chen Chen
Yi-Chun Chen
Wen-Ya Yu
Meng-Yen Ho
Chia-Yin Ho
Michael M.C. Lai
Wen-Chi Su
Publication date: 01-01-2021
Publisher: Springer Berlin Heidelberg
Keywords: Influenza Virus
Hypoxemia
Influenza
Biomarkers
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 1/2021
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-020-04008-1

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