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Pediatric Surgery International

Published in:

01-02-2018 | Original Article

Influence of stress factors on intestinal epithelial injury and regeneration

Authors: Carol Lee, Adam Minich, Bo Li, Hiromu Miyake, Shogo Seo, Agostino Pierro

Published in: Pediatric Surgery International | Issue 2/2018

Abstract

Purpose

Lgr5+ intestinal epithelial stem cells (ISCs) crucial for intestinal epithelial regeneration are impaired during necrotizing enterocolitis. This study aims to investigate the influence of different stressors on intestinal epithelial injury and regeneration in vitro.

Methods

Intestinal epithelial cells (IEC-18) were exposed to stressors such as lipopolysaccharide, hydrogen peroxide, and serum. Cell viability was assessed using MTT assay at 18 and 24 h. IL-6 and Lgr5 gene expressions were measured using qPCR.

Results

IEC-18 cell viability decreased 18 h following administration of lipopolysaccharide, hydrogen peroxide, and low serum concentration. However, after 24 h, the decrease in cell viability was observed only in higher, but not in lower concentrations of lipopolysaccharide and hydrogen peroxide. IL-6 expression increased in all groups compared to control. Lgr5 expression was up-regulated in cells exposed to a single stressor, but down-regulated when multiple stressors were administered.

Conclusion

Lipopolysaccharide, hydrogen peroxide, or low serum induced IEC-18 injury. The upregulation of Lgr5 expression after exposure to a single stressor suggests that minor injury to IEC-18 induces Lgr5+ ISCs to stimulate repair. Conversely, when IEC-18 cells were exposed to multiple stressors, Lgr5 expression was reduced. We speculate that this finding is similar to what happens in NEC when multiple stressors cause impairment of intestinal epithelium regeneration.

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Title: Influence of stress factors on intestinal epithelial injury and regeneration
Authors: Carol Lee
Adam Minich
Bo Li
Hiromu Miyake
Shogo Seo
Agostino Pierro
Publication date: 01-02-2018
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Surgery International / Issue 2/2018
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-017-4183-3

At a glance: The STEP trials

Springer Medicine

Influence of stress factors on intestinal epithelial injury and regeneration

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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