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International Journal of Colorectal Disease

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01-11-2015 | Original Article

Influence of gentamicin-coded PVDF suture material on the healing of intestinal anastomosis in a rat model

Authors: Dominik S. Schoeb, Christian D. Klink, Andreas Lambertz, Roman Eickhoff, Daniel Busch, Tom F. Ulmer, Ulf P. Neumann, Marcel Binnebösel

Published in: International Journal of Colorectal Disease | Issue 11/2015

Abstract

Purpose

Intestinal anastomosis is a fundamental procedure in general surgery and required to restore intestinal continuity following resection. The aim of this study was to evaluate whether a gentamicin-coated polyvinylidene fluoride (PVDF) suture material has beneficial effect on anastomotic healing.

Methods

Ninety Sprague-Dawley rats were divided into three groups: a PVDF-suture group, a gentamicin-coated PVDF (GPVDF)-suture group and a control group using Maxon® (polyglycolid-co-trimethylene carbonate). For each animal, a colonic anastomosis was performed. Ten animals from each group were sacrificed on postoperative days 3, 5, and 14. Measurements of anastomotic bursting pressure were performed on days 3 and 5. At each time, collagen type I/III ratio, MMP 2 and MMP-9 expression and the proliferation index (Ki67) were analyzed.

Results

In total, 90 animals underwent surgery without postoperative complications. Bursting strength in the GPVDF group was significantly elevated on day 5. Immunohistochemistry showed significant increase of the collagen type I/III ratio for PVDF and GPVDF on days 3 and 5. MMP2 was significantly increased for PVDF on days 3 and 5 and for GPVDF on day 5. The analysis of MMP9 revealed significant increase compared to control on day 3 and 5 (GPVDF) as well as on day 5 (PVDF). Staining for Ki67 revealed a significant elevation on postoperative day 3 for the PVDF and the GPVDF group.

Conclusions

The present data shows the feasibility of PVDF as suture material for colonic anastomosis and confirms the ability of gentamicin to increase the stability of colonic anastomosis when used as coating material.

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Title: Influence of gentamicin-coded PVDF suture material on the healing of intestinal anastomosis in a rat model
Authors: Dominik S. Schoeb
Christian D. Klink
Andreas Lambertz
Roman Eickhoff
Daniel Busch
Tom F. Ulmer
Ulf P. Neumann
Marcel Binnebösel
Publication date: 01-11-2015
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Colorectal Disease / Issue 11/2015
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-015-2345-0

At a glance: The STEP trials

Springer Medicine

Influence of gentamicin-coded PVDF suture material on the healing of intestinal anastomosis in a rat model

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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