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01-02-2012 | Hepatobiliary Tumors

Infiltrating Memory/Senescent T Cell Ratio Predicts Extrahepatic Metastasis of Hepatocellular Carcinoma

Authors: Qiang Gao, MD, PhD, Jian Zhou, MD, PhD, Xiao-Ying Wang, MD, PhD, Shuang-Jian Qiu, MD, PhD, Kang Song, MD, PhD, Xiao-Wu Huang, MD, PhD, Jian Sun, MD, PhD, Ying-Hong Shi, MD, PhD, Bai-Zhou Li, MD, PhD, Yong-Sheng Xiao, MD, PhD, Jia Fan, MD, PhD

Published in: Annals of Surgical Oncology | Issue 2/2012

Abstract

Background

The density of tumor-infiltrating immunocytes (TICs) has been proposed as an independent predictor of intrahepatic recurrence in patients with hepatocellular carcinoma (HCC). However, the relative roles of TIC density in predicting tumor extrahepatic metastasis remain to be elucidated.

Methods

The densities of CD3⁺, CD8⁺, granzyme B⁺, FoxP3⁺, CD45RO⁺, CD20⁺, CD1a⁺, CD83⁺, CD57⁺, and CD68⁺ TICs were assessed by immunohistochemistry in tissue microarrays containing paired intratumoral (IT) and peritumoral (PT) tissues from 206 consecutive HCC patients who underwent liver transplantation. Occurrence of extrahepatic metastasis, recurrence-free survival (RFS), and cancer-specific survival (CSS) were assessed retrospectively in relation to TIC densities.

Results

CD45RO⁺ memory T cell density was lower in tumor tissue compared with peritumor, whereas CD57⁺ senescent T cell density was higher. Univariate analysis revealed that increased CD45RO _IT ⁺ and decreased CD57 _PT ⁺ densities were statistically significantly associated with favorable RFS and CSS, while other types of TICs, intratumorally or peritumorally, showed no prognostic values. Further, the CD45RO _IT ⁺ /CD57 _PT ⁺ ratio could stratify patients more accurately in terms of RFS and CSS than either marker used alone. Finally, multivariate analysis indicated that a high CD45RO _IT ⁺ /CD57 _PT ⁺ ratio was independently associated with better RFS (hazard ratio [HR] = 0.64; 95% confidence interval [CI], 0.42 to 0.98; P = 0.040) and CSS (HR = 0.51; 95% CI, 0.31 to 0.83; P = 0.007), but not CD45RO _IT ⁺ or CD57 _PT ⁺ individually.

Conclusions

These results suggest that the CD45RO _IT ⁺ /CD57 _PT ⁺ (memory/senescent T cell) ratio is of vital importance in preventing HCC extrahepatic metastasis and in particular demonstrates its independent prognostic value in liver transplant recipients.

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Title: Infiltrating Memory/Senescent T Cell Ratio Predicts Extrahepatic Metastasis of Hepatocellular Carcinoma
Authors: Qiang Gao, MD, PhD
Jian Zhou, MD, PhD
Xiao-Ying Wang, MD, PhD
Shuang-Jian Qiu, MD, PhD
Kang Song, MD, PhD
Xiao-Wu Huang, MD, PhD
Jian Sun, MD, PhD
Ying-Hong Shi, MD, PhD
Bai-Zhou Li, MD, PhD
Yong-Sheng Xiao, MD, PhD
Jia Fan, MD, PhD
Publication date: 01-02-2012
Publisher: Springer-Verlag
Published in: Annals of Surgical Oncology / Issue 2/2012
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI: https://doi.org/10.1245/s10434-011-1864-3

At a glance: The STEP trials

Springer Medicine

Infiltrating Memory/Senescent T Cell Ratio Predicts Extrahepatic Metastasis of Hepatocellular Carcinoma

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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