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01-02-2017 | Short Communication

Induction of varicella zoster virus DNA replication in dissociated human trigeminal ganglia

Authors: Randall J. Cohrs, Hussain Badani, Nicholas L. Baird, Teresa M. White, Bridget Sanford, Don Gilden

Published in: Journal of NeuroVirology | Issue 1/2017

Abstract

Varicella zoster virus (VZV), a human neurotropic alphaherpesvirus, becomes latent after primary infection and reactivates to produce zoster. To study VZV latency and reactivation, human trigeminal ganglia removed within 24 h after death were mechanically dissociated, randomly distributed into six-well tissue culture plates and incubated with reagents to inactivate nerve growth factor (NGF) or phosphoinositide 3-kinase (PI3-kinase) pathways. At 5 days, VZV DNA increased in control and PI3-kinase inhibitor-treated cultures to the same extent, but was significantly more abundant in anti-NGF-treated cultures (p = 0.001). Overall, VZV DNA replication is regulated in part by an NGF pathway that is PI3-kinase-independent.

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Title: Induction of varicella zoster virus DNA replication in dissociated human trigeminal ganglia
Authors: Randall J. Cohrs
Hussain Badani
Nicholas L. Baird
Teresa M. White
Bridget Sanford
Don Gilden
Publication date: 01-02-2017
Publisher: Springer International Publishing
Published in: Journal of NeuroVirology / Issue 1/2017
Print ISSN: 1355-0284
Electronic ISSN: 1538-2443
DOI: https://doi.org/10.1007/s13365-016-0480-1

At a glance: The STEP trials

Springer Medicine

Induction of varicella zoster virus DNA replication in dissociated human trigeminal ganglia

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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