Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
European Journal of Nuclear Medicine and Molecular Imaging
Published in: European Journal of Nuclear Medicine and Molecular Imaging 7/2012

01-07-2012 | Short Communication

In vivo experimental imaging of osteochondral defects and their healing using 99mTc-NTP 15-5 radiotracer

Authors: E. Miot-Noirault, J. Guicheux, A. Vidal, O. Gauthier, P. Auzeloux, J. Lesoeur, F. Cachin, S. Askienazy, J. M. Chezal, C. Vinatier

Published in: European Journal of Nuclear Medicine and Molecular Imaging | Issue 7/2012

Login to get access

Abstract

Purpose

A rabbit model of osteochondral defects (OD) and spontaneous healing was longitudinally followed over 12 weeks, by in vivo joint scintigraphy using 99mTc-NTP 15-5, and histology.

Methods

We used two models, one with one OD (OD1 group) in the femoral condyle of one knee and the other with two ODs (OD2 group) in the femoral condyle of one knee, with the contralateral knees serving as the reference. A serial longitudinal imaging study was performed with the scintigraphic ratio (SR, operated knee uptake/contralateral knee uptake) determined at each time-point.

Results

ODs were imaged as radioactive defects. The SR was decreased with respective to controls, with values of 0.73 ± 0.08 and 0.65 ± 0.07 in the OD1 and OD2 groups, respectively, at 4 weeks after surgery. Histology of both OD groups revealed the presence of repair tissue characterized by a small amount of sulphated glycosaminoglycans and collagen.

Conclusion

99mTc-NTP 15-5 imaging provided quantitative criteria useful for in vivo evaluation of cartilage trauma and healing.
Literature
1.
Bedi A, Feeley BT, Williams 3rd RJ. Management of articular cartilage defects of the knee. J Bone Joint Surg Am. 2010;92:994–1009.PubMedCrossRef
2.
Yokota M, Yasuda K, Kitamura N, Arakaki K, Onodera S, Kurokawa T, et al. Spontaneous hyaline cartilage regeneration can be induced in an osteochondral defect created in the femoral condyle using a novel double network hydrogel. BMC Musculoskelet Disord. 2011;12:49–59.PubMedCrossRef
3.
Vinatier C, Mrugala D, Jorgensen C, Guicheux J, Noel D. Cartilage engineering: a crucial combination of cells, biomaterials and biofactors. Trends Biotechnol. 2009;27:307–14.PubMedCrossRef
4.
Ollier M, Maurizis JC, Nicolas C, Bonafous J, de Latour M, Veyre A, et al. Joint scintigraphy in rabbits with 99mTc-N-[3-(triethylammonio) propyl]-15ane-N5, a new radiodiagnostic agent for articular cartilage imaging. J Nucl Med. 2001;42:141–5.PubMed
5.
Miot-Noirault E, Vidal A, Pastoureau P, Bonafous J, Chomel A, Sarry L, et al. Early detection and monitoring of cartilage alteration in the experimental meniscectomized guinea pig of osteoarthritis by 99mTc-NTP 15-5 scintigraphy. Eur J Nucl Med Mol Imaging. 2007;34:1280–90.PubMedCrossRef
6.
Vinatier C, Gauthier O, Fatimi A, Merceron C, Masson M, Moreau A, et al. An injectable cellulose-based hydrogel for the transfer of autologous nasal chondrocytes in articular cartilage defects. Biotechnol Bioeng. 2009;102:1259–67.PubMedCrossRef
7.
Cachin F, Boisgard S, Vidal A, Filaire M, Auzeloux P, Culot D, et al. First ex vivo study demonstrating that 99mTc-NTP 15-5 radiotracer binds to human articular cartilage. Eur J Nucl Med Mol Imaging. 2011;38:2077–82.PubMedCrossRef
8.
Vanderheyden JL. The use of imaging in preclinical drug development. Q J Nucl Med Mol Imaging. 2009;53:374–81.PubMed
Metadata
Title
In vivo experimental imaging of osteochondral defects and their healing using 99mTc-NTP 15-5 radiotracer
Authors
E. Miot-Noirault
J. Guicheux
A. Vidal
O. Gauthier
P. Auzeloux
J. Lesoeur
F. Cachin
S. Askienazy
J. M. Chezal
C. Vinatier
Publication date
01-07-2012
Publisher
Springer-Verlag
Published in
European Journal of Nuclear Medicine and Molecular Imaging / Issue 7/2012
Print ISSN: 1619-7070
Electronic ISSN: 1619-7089
DOI
https://doi.org/10.1007/s00259-012-2081-4

Other articles of this Issue 7/2012

European Journal of Nuclear Medicine and Molecular Imaging 7/2012 Go to the issue

Image of the Month

Skin lesions, elevated serum lipase level and abnormal bone scintigraphy

Book Review

Blaine T. Smith and Kara D. Weatherman (eds): Diagnostic Imaging for Pharmacists

Society Communications

Even in times of crisis the distribution of knowledge is …

Original Article

Preclinical evaluation of two 68Ga-siderophores as potential radiopharmaceuticals for Aspergillus fumigatus infection imaging

Original Article

LV dyssynchrony as assessed by phase analysis of gated SPECT myocardial perfusion imaging in patients with Wolff-Parkinson-White syndrome

Original Article

Predictive value of early and late residual 18F-fluorodeoxyglucose and 18F-fluorothymidine uptake using different SUV measurements in patients with non-small-cell lung cancer treated with erlotinib