Skip to main content
Top
Published in: Calcified Tissue International 5/2011

01-05-2011 | Original Research

In Vivo CT Quantification of Trabecular Bone Dynamics in Mice after Sciatic Neurectomy Using Monochromatic Synchrotron Radiation

Authors: Takeshi Matsumoto, Ken Nishikawa, Masao Tanaka, Kentaro Uesugi

Published in: Calcified Tissue International | Issue 5/2011

Login to get access

Abstract

We demonstrated the capability of in vivo synchrotron radiation CT (SRCT) in analyzing short-term changes in trabecular bone architecture (TBA) and the degree of bone mineralization (DBM) in small animals. Mice underwent unilateral sciatic neurectomy (SN) and sham operation on the contralateral side (SO) at 13 weeks of age. In vivo SRCT scans (11.7-μm cubic voxel) were made of both knees 7 and 17 days (group 1, n = 7) or only 17 days (group 2, n = 6) after surgery. In three mice in group 2, one knee was scanned twice on the same day in different orientations for reproducibility testing. Two scan data sets of the tibial proximal metaphysis acquired at different time points (group 1) or at the same time point (group 2) were registered for detecting differences in volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), connectivity density (Conn.D), and mean DBM (mDBM). The reproducibility test showed small errors of <2.5% in the TBA indexes and <3.0% in mDBM, while mismatched bone regions amounted to >25%. In group 1, Tb.Th increased but Tb.N and Conn.D decreased in both SN and SO; BV/TV and mDBM increased only in SO; accordingly, BV/TV, Tb.Th, and mDBM became lower in SN than in SO. No significant interaction between SN and irradiation was found; the SN effects on TBA and DBM were similar between groups 1 and 2, although synchrotron irradiation led to higher Tb.Th and lower Tb.N in group 1. In conclusion, in vivo SRCT has potential use for detecting short-term bone dynamics of small animals.
Appendix
Available only for authorised users
Literature
1.
go back to reference Dempster DW (2000) The contribution of trabecular architecture to cancellous bone quality. J Bone Miner Res 15:20–23PubMedCrossRef Dempster DW (2000) The contribution of trabecular architecture to cancellous bone quality. J Bone Miner Res 15:20–23PubMedCrossRef
2.
3.
go back to reference Liu XS, Sajda P, Saha PK, Wehrli FW, Guo XE (2006) Quantification of the roles of trabecular microarchitecture and trabecular type in determining the elastic modulus of human trabecular bone. J Bone Miner Res 21:1608–1617PubMedCrossRef Liu XS, Sajda P, Saha PK, Wehrli FW, Guo XE (2006) Quantification of the roles of trabecular microarchitecture and trabecular type in determining the elastic modulus of human trabecular bone. J Bone Miner Res 21:1608–1617PubMedCrossRef
4.
go back to reference Niebur GL, Feldstein MJ, Yuen JC, Chen TJ, Keaveny TM (2000) High-resolution finite element models with tissue strength asymmetry accurately predict failure of trabecular bone. J Biomech 33:1575–1583PubMedCrossRef Niebur GL, Feldstein MJ, Yuen JC, Chen TJ, Keaveny TM (2000) High-resolution finite element models with tissue strength asymmetry accurately predict failure of trabecular bone. J Biomech 33:1575–1583PubMedCrossRef
5.
go back to reference Van Der Linden JC, Verhaar JA, Weinans H (2001) A three-dimensional simulation of age-related remodeling in trabecular bone. J Bone Miner Res 16:688–696CrossRef Van Der Linden JC, Verhaar JA, Weinans H (2001) A three-dimensional simulation of age-related remodeling in trabecular bone. J Bone Miner Res 16:688–696CrossRef
6.
go back to reference Ciarelli TE, Fyhrie DP, Schaffler MB, Goldstein SA (2000) Variations in three-dimensional cancellous bone architecture of the proximal femur in female hip fractures and in controls. J Bone Miner Res 15:32–40PubMedCrossRef Ciarelli TE, Fyhrie DP, Schaffler MB, Goldstein SA (2000) Variations in three-dimensional cancellous bone architecture of the proximal femur in female hip fractures and in controls. J Bone Miner Res 15:32–40PubMedCrossRef
7.
go back to reference Homminga J, McCreadie BR, Ciarelli TE, Weinans H, Goldstein SA, Huiskes R (2002) Cancellous bone mechanical properties from normals and patients with hip fractures differ on the structure level, not on the bone hard tissue level. Bone 30:759–764PubMedCrossRef Homminga J, McCreadie BR, Ciarelli TE, Weinans H, Goldstein SA, Huiskes R (2002) Cancellous bone mechanical properties from normals and patients with hip fractures differ on the structure level, not on the bone hard tissue level. Bone 30:759–764PubMedCrossRef
8.
go back to reference McDonnell P, McHugh PE, O’Mahoney D (2007) Vertebral osteoporosis and trabecular bone quality. Ann Biomed Eng 35:170–189PubMedCrossRef McDonnell P, McHugh PE, O’Mahoney D (2007) Vertebral osteoporosis and trabecular bone quality. Ann Biomed Eng 35:170–189PubMedCrossRef
9.
go back to reference Stauber M, Rapillard L, van Lenthe GH, Zysset P, Müller R (2006) Importance of individual rods and plates in the assessment of bone quality and their contribution to bone stiffness. J Bone Miner Res 21:586–589PubMedCrossRef Stauber M, Rapillard L, van Lenthe GH, Zysset P, Müller R (2006) Importance of individual rods and plates in the assessment of bone quality and their contribution to bone stiffness. J Bone Miner Res 21:586–589PubMedCrossRef
10.
go back to reference Hildebrand T, Ruegsegger P (1997) A new method for the model-independent assessment of thickness in three-dimensional images. J Microsc 185:67–75CrossRef Hildebrand T, Ruegsegger P (1997) A new method for the model-independent assessment of thickness in three-dimensional images. J Microsc 185:67–75CrossRef
11.
go back to reference Odgaard A (1997) Three-dimensional methods for quantification of cancellous bone architecture. Bone 20:315–328PubMedCrossRef Odgaard A (1997) Three-dimensional methods for quantification of cancellous bone architecture. Bone 20:315–328PubMedCrossRef
12.
go back to reference Boyd SK, Davison P, Müller R, Gasser JA (2006) Monitoring individual morphological changes over time in ovariectomized rats by in vivo micro-computed tomography. Bone 39:854–862PubMedCrossRef Boyd SK, Davison P, Müller R, Gasser JA (2006) Monitoring individual morphological changes over time in ovariectomized rats by in vivo micro-computed tomography. Bone 39:854–862PubMedCrossRef
13.
go back to reference David V, Laroche N, Boudignon B, Lafage-Proust MH, Alexandre C, Ruegsegger P, Vico L (2003) Noninvasive in vivo monitoring of bone architecture alterations in hindlimb-unloaded female rats using novel three-dimensional microcomputed tomography. J Bone Miner Res 18:1622–1631PubMedCrossRef David V, Laroche N, Boudignon B, Lafage-Proust MH, Alexandre C, Ruegsegger P, Vico L (2003) Noninvasive in vivo monitoring of bone architecture alterations in hindlimb-unloaded female rats using novel three-dimensional microcomputed tomography. J Bone Miner Res 18:1622–1631PubMedCrossRef
14.
go back to reference Ibiwoye MO, Powell KA, Grabiner MD, Patterson TE, Sakai Y, Zborowski M, Wolfman A, Midura RJ (2004) Bone mass is preserved in a critical-sized osteotomy by low energy pulsed electromagnetic fields as quantitated by in vivo micro-computed tomography. J Orthop Res 22:1086–1093PubMedCrossRef Ibiwoye MO, Powell KA, Grabiner MD, Patterson TE, Sakai Y, Zborowski M, Wolfman A, Midura RJ (2004) Bone mass is preserved in a critical-sized osteotomy by low energy pulsed electromagnetic fields as quantitated by in vivo micro-computed tomography. J Orthop Res 22:1086–1093PubMedCrossRef
15.
go back to reference Waarsing JH, Day JS, Verhaar JA, Ederveen AG, Weinans H (2006) Bone loss dynamics result in trabecular alignment in aging and ovariectomized rats. J Orthop Res 24:926–935PubMedCrossRef Waarsing JH, Day JS, Verhaar JA, Ederveen AG, Weinans H (2006) Bone loss dynamics result in trabecular alignment in aging and ovariectomized rats. J Orthop Res 24:926–935PubMedCrossRef
16.
go back to reference Waarsing JH, Day JS, van der Linden JC, Ederveen AG, Spanjers C, De Clerck N, Sasov A, Verhaar JA, Weinans H (2004) Detecting and local changes in the tibiae of individual rats: a novel method to analyse longitudinal in vivo micro-CT data. Bone 34:163–169PubMedCrossRef Waarsing JH, Day JS, van der Linden JC, Ederveen AG, Spanjers C, De Clerck N, Sasov A, Verhaar JA, Weinans H (2004) Detecting and local changes in the tibiae of individual rats: a novel method to analyse longitudinal in vivo micro-CT data. Bone 34:163–169PubMedCrossRef
17.
go back to reference Boyd SK, Moser S, Kuhn M, Klinck RJ, Krauze PL, Müller R, Gasser JA (2006) Evaluation of three-dimensional image registration methodologies for in vivo micro-computed tomography. Ann Biomed Eng 34:1587–1599PubMedCrossRef Boyd SK, Moser S, Kuhn M, Klinck RJ, Krauze PL, Müller R, Gasser JA (2006) Evaluation of three-dimensional image registration methodologies for in vivo micro-computed tomography. Ann Biomed Eng 34:1587–1599PubMedCrossRef
18.
go back to reference Pauchard Y, Mattmann C, Kuhn A, Gasser JA, Boyd SK (2008) Using temporal trends of 3D bone micro-architecture to predict bone quality. J Biomech 41:2946–2953PubMedCrossRef Pauchard Y, Mattmann C, Kuhn A, Gasser JA, Boyd SK (2008) Using temporal trends of 3D bone micro-architecture to predict bone quality. J Biomech 41:2946–2953PubMedCrossRef
19.
go back to reference Bonse U, Busch F (1996) X-ray computed microtomography (microCT) using synchrotron radiation (SR). Prog Biophys Mol Biol 65:133–169PubMedCrossRef Bonse U, Busch F (1996) X-ray computed microtomography (microCT) using synchrotron radiation (SR). Prog Biophys Mol Biol 65:133–169PubMedCrossRef
20.
go back to reference Suortti P, Thomlinson W (2003) Medical applications of synchrotron radiation. Phys Med Biol 48:R1–R35PubMedCrossRef Suortti P, Thomlinson W (2003) Medical applications of synchrotron radiation. Phys Med Biol 48:R1–R35PubMedCrossRef
21.
go back to reference Borah B, Dufresne TE, Ritman EL, Jorgensen SM, Liu S, Chmielewski PA, Phipps RJ, Zhou X, Sibonga JD, Turner RT (2006) Long-term risedronate treatment normalizes mineralization and continues to preserve trabecular architecture: sequential triple biopsy studies with micro-computed tomography. Bone 39:345–352PubMedCrossRef Borah B, Dufresne TE, Ritman EL, Jorgensen SM, Liu S, Chmielewski PA, Phipps RJ, Zhou X, Sibonga JD, Turner RT (2006) Long-term risedronate treatment normalizes mineralization and continues to preserve trabecular architecture: sequential triple biopsy studies with micro-computed tomography. Bone 39:345–352PubMedCrossRef
22.
go back to reference Ito M, Ejiri S, Jinnai H, Kono J, Ikeda S, Nishida A, Uesugi K, Yagi N, Tanaka M, Hayashi K (2003) Bone structure and mineralization demonstrated using synchrotron radiation computed tomography (SR-CT) in animal models: preliminary findings. J Bone Miner Metab 21:287–293PubMedCrossRef Ito M, Ejiri S, Jinnai H, Kono J, Ikeda S, Nishida A, Uesugi K, Yagi N, Tanaka M, Hayashi K (2003) Bone structure and mineralization demonstrated using synchrotron radiation computed tomography (SR-CT) in animal models: preliminary findings. J Bone Miner Metab 21:287–293PubMedCrossRef
23.
go back to reference Matsumoto T, Yoshino M, Uesugi K, Tanaka M (2007) Biphasic change and disuse-mediated regression of canal network structure in cortical bone of growing rats. Bone 41:239–246PubMedCrossRef Matsumoto T, Yoshino M, Uesugi K, Tanaka M (2007) Biphasic change and disuse-mediated regression of canal network structure in cortical bone of growing rats. Bone 41:239–246PubMedCrossRef
24.
go back to reference Nuzzo S, Peyrin F, Cloetens P, Baruchel J, Boivin G (2002) Quantification of the degree of mineralization of bone in three dimensions using synchrotron radiation microtomography. Med Phys 29:2672–2681PubMedCrossRef Nuzzo S, Peyrin F, Cloetens P, Baruchel J, Boivin G (2002) Quantification of the degree of mineralization of bone in three dimensions using synchrotron radiation microtomography. Med Phys 29:2672–2681PubMedCrossRef
25.
go back to reference Schneider P, Stauber M, Voide R, Stampanoni M, Donahue LR, Müller R (2007) Ultrastructural properties in cortical bone vary greatly in two inbred strains of mice as assessed by synchrotron light based micro- and nano-CT. J Bone Miner Res 22:1557–1570PubMedCrossRef Schneider P, Stauber M, Voide R, Stampanoni M, Donahue LR, Müller R (2007) Ultrastructural properties in cortical bone vary greatly in two inbred strains of mice as assessed by synchrotron light based micro- and nano-CT. J Bone Miner Res 22:1557–1570PubMedCrossRef
26.
go back to reference Sone T, Tamada T, Jo Y, Miyoshi H, Fukunaga M (2004) Analysis of three-dimensional microarchitecture and degree of mineralization in bone metastases from prostate cancer using synchrotron microcomputed tomography. Bone 35:432–438PubMedCrossRef Sone T, Tamada T, Jo Y, Miyoshi H, Fukunaga M (2004) Analysis of three-dimensional microarchitecture and degree of mineralization in bone metastases from prostate cancer using synchrotron microcomputed tomography. Bone 35:432–438PubMedCrossRef
27.
go back to reference Yao W, Balooch G, Balooch M, Jiang Y, Nalla RK, Kinney J, Wronski TJ, Lane NE (2006) Sequential treatment of ovariectomized mice with bFGF and risedronate restored trabecular bone microarchitecture and mineralization. Bone 39:460–469PubMedCrossRef Yao W, Balooch G, Balooch M, Jiang Y, Nalla RK, Kinney J, Wronski TJ, Lane NE (2006) Sequential treatment of ovariectomized mice with bFGF and risedronate restored trabecular bone microarchitecture and mineralization. Bone 39:460–469PubMedCrossRef
28.
go back to reference Bayat S, Apostol L, Boller E, Brochard T, Peyrin F (2005) In vivo imaging of bone micro-architecture in mice with 3D synchrotron radiation micro-tomography. Nucl Instrum Methods Phys Res A Accel Spectrom Detect Assoc Equip 548:247–252CrossRef Bayat S, Apostol L, Boller E, Brochard T, Peyrin F (2005) In vivo imaging of bone micro-architecture in mice with 3D synchrotron radiation micro-tomography. Nucl Instrum Methods Phys Res A Accel Spectrom Detect Assoc Equip 548:247–252CrossRef
29.
go back to reference Kinney JH, Lane NE, Haupt DL (1995) In vivo, three-dimensional microscopy of trabecular bone. J Bone Miner Res 10:264–270PubMedCrossRef Kinney JH, Lane NE, Haupt DL (1995) In vivo, three-dimensional microscopy of trabecular bone. J Bone Miner Res 10:264–270PubMedCrossRef
30.
go back to reference Bushberg JT, Seibert JA, Leidholdt EM Jr, Boone JM (2001) The essential physics of medical imaging, 2nd edn. Lippincott Williams & Wilkins, Philadelphia Bushberg JT, Seibert JA, Leidholdt EM Jr, Boone JM (2001) The essential physics of medical imaging, 2nd edn. Lippincott Williams & Wilkins, Philadelphia
31.
go back to reference Hamilton SA, Pecaut MJ, Gridley DS, Travis ND, Bandstra ER, Willey JS, Nelson GA, Bateman TA (2006) A murine model for bone loss from therapeutic and space-relevant sources of radiation. J Appl Physiol 101:789–793PubMedCrossRef Hamilton SA, Pecaut MJ, Gridley DS, Travis ND, Bandstra ER, Willey JS, Nelson GA, Bateman TA (2006) A murine model for bone loss from therapeutic and space-relevant sources of radiation. J Appl Physiol 101:789–793PubMedCrossRef
32.
go back to reference Jacobsson M, Jönsson A, Albrektsson T, Turesson I (1985) Alterations in bone regenerative capacity after low level gamma irradiation. A quantitative study. Scand J Plast Reconstr Surg 19:231–236PubMedCrossRef Jacobsson M, Jönsson A, Albrektsson T, Turesson I (1985) Alterations in bone regenerative capacity after low level gamma irradiation. A quantitative study. Scand J Plast Reconstr Surg 19:231–236PubMedCrossRef
33.
go back to reference Goto S, Takeshita K, Suzuki Y, Ohashi H, Asano Y, Kimura H, Matsushita T, Yagi N, Isshiki M, Yamazaki H, Yoneda Y, Umetani K, Ishikawa T (2001) Construction and commissioning of a 215-m-long beamline at SPring-8. Nucl Instrum Methods Phys Res A Accel Spectrom Detect Assoc Equip 467–468:682–685CrossRef Goto S, Takeshita K, Suzuki Y, Ohashi H, Asano Y, Kimura H, Matsushita T, Yagi N, Isshiki M, Yamazaki H, Yoneda Y, Umetani K, Ishikawa T (2001) Construction and commissioning of a 215-m-long beamline at SPring-8. Nucl Instrum Methods Phys Res A Accel Spectrom Detect Assoc Equip 467–468:682–685CrossRef
34.
go back to reference Maes F, Collignon A, Vander meulen D, Marchal G, Suetens P (1997) Multimodality image registration by maximization of mutual information. IEEE Trans Med Imaging 16:187–198PubMedCrossRef Maes F, Collignon A, Vander meulen D, Marchal G, Suetens P (1997) Multimodality image registration by maximization of mutual information. IEEE Trans Med Imaging 16:187–198PubMedCrossRef
35.
go back to reference Studholme C, Hill DL, Hawkes DJ (1997) Automated three-dimensional registration of magnetic resonance and positron emission tomography brain images by multiresolution optimization of voxel similarity measures. Med Phys 24:25–35PubMedCrossRef Studholme C, Hill DL, Hawkes DJ (1997) Automated three-dimensional registration of magnetic resonance and positron emission tomography brain images by multiresolution optimization of voxel similarity measures. Med Phys 24:25–35PubMedCrossRef
36.
go back to reference Toriwaki J, Mori K (2001) Distance transformation and skeletonization of 3D pictures and their applications to medical images. In: Bertrand G, Imiya A, Klette R (eds) Digital and image geometry. Springer, Tokyo, pp 412–428CrossRef Toriwaki J, Mori K (2001) Distance transformation and skeletonization of 3D pictures and their applications to medical images. In: Bertrand G, Imiya A, Klette R (eds) Digital and image geometry. Springer, Tokyo, pp 412–428CrossRef
37.
go back to reference Voor MJ, Yang S, Burden RL, Waddell SW (2008) In vivo micro-CT scanning of a rabbit distal femur: repeatability and reproducibility. J Biomech 41:186–193PubMedCrossRef Voor MJ, Yang S, Burden RL, Waddell SW (2008) In vivo micro-CT scanning of a rabbit distal femur: repeatability and reproducibility. J Biomech 41:186–193PubMedCrossRef
38.
go back to reference Iwamoto J, Takeda T, Ichimura S, Sato Y, Yeh JK (2003) Comparative effects of orchidectomy and sciatic neurectomy on cortical and cancellous bone in young growing rats. J Bone Miner Metab 21:211–216PubMed Iwamoto J, Takeda T, Ichimura S, Sato Y, Yeh JK (2003) Comparative effects of orchidectomy and sciatic neurectomy on cortical and cancellous bone in young growing rats. J Bone Miner Metab 21:211–216PubMed
39.
go back to reference Ito M, Nishida A, Nakamura T, Uetani M, Hayashi K (2002) Differences of three-dimensional trabecular microstructure in osteopenic rat models caused by ovariectomy and neurectomy. Bone 30:594–598PubMedCrossRef Ito M, Nishida A, Nakamura T, Uetani M, Hayashi K (2002) Differences of three-dimensional trabecular microstructure in osteopenic rat models caused by ovariectomy and neurectomy. Bone 30:594–598PubMedCrossRef
40.
go back to reference Zeng QQ, Jee WS, Bigornia AE, King JG Jr, D’Souza SM, Li XJ, Ma YF, Wechter WJ (1996) Time responses of cancellous and cortical bones to sciatic neurectomy in growing female rats. Bone 19:13–21PubMedCrossRef Zeng QQ, Jee WS, Bigornia AE, King JG Jr, D’Souza SM, Li XJ, Ma YF, Wechter WJ (1996) Time responses of cancellous and cortical bones to sciatic neurectomy in growing female rats. Bone 19:13–21PubMedCrossRef
41.
go back to reference Brouwers JE, Lambers FM, van Rietbergen B, Ito K, Huiskes R (2009) Comparison of bone loss induced by ovariectomy and neurectomy in rats analyzed by in vivo micro-CT. J Orthop Res 27:1521–1527PubMedCrossRef Brouwers JE, Lambers FM, van Rietbergen B, Ito K, Huiskes R (2009) Comparison of bone loss induced by ovariectomy and neurectomy in rats analyzed by in vivo micro-CT. J Orthop Res 27:1521–1527PubMedCrossRef
42.
go back to reference Klinck RJ, Campbell GM, Boyd SK (2008) Radiation effects on bone architecture in mice and rats resulting from in vivo micro-computed tomography scanning. Med Eng Phys 30:888–895PubMedCrossRef Klinck RJ, Campbell GM, Boyd SK (2008) Radiation effects on bone architecture in mice and rats resulting from in vivo micro-computed tomography scanning. Med Eng Phys 30:888–895PubMedCrossRef
43.
go back to reference Kazakia GJ, Burghardt AJ, Cheung S, Majumdar S (2008) Assessment of bone tissue mineralization by conventional X-ray microcomputed tomography: comparison with synchrotron radiation microcomputed tomography and ash measurements. Med Phys 35:3170–3179PubMedCrossRef Kazakia GJ, Burghardt AJ, Cheung S, Majumdar S (2008) Assessment of bone tissue mineralization by conventional X-ray microcomputed tomography: comparison with synchrotron radiation microcomputed tomography and ash measurements. Med Phys 35:3170–3179PubMedCrossRef
44.
go back to reference Beamer WG, Donahue LR, Rosen CJ, Baylink DJ (1996) Genetic variability in adult bone density among inbred strains of mice. Bone 18:397–403PubMedCrossRef Beamer WG, Donahue LR, Rosen CJ, Baylink DJ (1996) Genetic variability in adult bone density among inbred strains of mice. Bone 18:397–403PubMedCrossRef
45.
go back to reference Sheng MH, Baylink DJ, Beamer WG, Donahue LR, Rosen CJ, Lau KH, Wergedal JE (1999) Histomorphometric studies show that bone formation and bone mineral apposition rates are greater in C3H/HeJ (high-density) than C57BL/6 J (low-density) mice during growth. Bone 25:421–429PubMedCrossRef Sheng MH, Baylink DJ, Beamer WG, Donahue LR, Rosen CJ, Lau KH, Wergedal JE (1999) Histomorphometric studies show that bone formation and bone mineral apposition rates are greater in C3H/HeJ (high-density) than C57BL/6 J (low-density) mice during growth. Bone 25:421–429PubMedCrossRef
46.
go back to reference Matsumura S, Jikko A, Hiranuma H, Deguchi A, Fuchihata H (1996) Effect of X-ray irradiation on proliferation and differentiation of osteoblast. Calcif Tissue Int 59:307–308PubMedCrossRef Matsumura S, Jikko A, Hiranuma H, Deguchi A, Fuchihata H (1996) Effect of X-ray irradiation on proliferation and differentiation of osteoblast. Calcif Tissue Int 59:307–308PubMedCrossRef
47.
go back to reference Dare A, Hachisu R, Yamaguchi A, Yokose S, Yoshiki S, Okano T (1997) Effects of ionizing radiation on proliferation and differentiation of osteoblast-like cells. J Dent Res 76:658–664PubMedCrossRef Dare A, Hachisu R, Yamaguchi A, Yokose S, Yoshiki S, Okano T (1997) Effects of ionizing radiation on proliferation and differentiation of osteoblast-like cells. J Dent Res 76:658–664PubMedCrossRef
48.
go back to reference Brouwers JE, van Rietbergen B, Huiskes R (2007) No effects of in vivo micro-CT radiation on structural parameters and bone marrow cells in proximal tibia of Wistar rats detected after eight weekly scans. J Orthop Res 25:1325–1332PubMedCrossRef Brouwers JE, van Rietbergen B, Huiskes R (2007) No effects of in vivo micro-CT radiation on structural parameters and bone marrow cells in proximal tibia of Wistar rats detected after eight weekly scans. J Orthop Res 25:1325–1332PubMedCrossRef
49.
go back to reference Ford NL, Thornton MM, Holdsworth DW (2003) Fundamental image quality limits for microcomputed tomography in small animals. Med Phys 30:2869–2877PubMedCrossRef Ford NL, Thornton MM, Holdsworth DW (2003) Fundamental image quality limits for microcomputed tomography in small animals. Med Phys 30:2869–2877PubMedCrossRef
Metadata
Title
In Vivo CT Quantification of Trabecular Bone Dynamics in Mice after Sciatic Neurectomy Using Monochromatic Synchrotron Radiation
Authors
Takeshi Matsumoto
Ken Nishikawa
Masao Tanaka
Kentaro Uesugi
Publication date
01-05-2011
Publisher
Springer-Verlag
Published in
Calcified Tissue International / Issue 5/2011
Print ISSN: 0171-967X
Electronic ISSN: 1432-0827
DOI
https://doi.org/10.1007/s00223-011-9475-3

Other articles of this Issue 5/2011

Calcified Tissue International 5/2011 Go to the issue
Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.