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European Journal of Nuclear Medicine and Molecular Imaging

Published in:

01-03-2008

In vivo calcium imaging of the aging and diseased brain

Authors: Gerhard Eichhoff, Marc Aurel Busche, Olga Garaschuk

Published in: European Journal of Nuclear Medicine and Molecular Imaging | Special Issue 1/2008

Abstract

Purpose

Over the last decade, in vivo calcium imaging became a powerful tool for studying brain function. With the use of two-photon microscopy and modern labelling techniques, it allows functional studies of individual living cells, their processes and their interactions within neuronal networks. In vivo calcium imaging is even more important for studying the aged brain, which is hard to investigate in situ due to the fragility of neuronal tissue.

Methods

In this article, we give a brief overview of the techniques applicable to image aged rodent brain at cellular resolution.

Results

We use multicolor imaging to visualize specific cell types (neurons, astrocytes, microglia) as well as the autofluorescence of the “aging pigment” lipofuscin.

Conclusions

Further, we illustrate an approach for simultaneous imaging of cortical cells and senile plaques in mouse models of Alzheimer’s disease.

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Title: In vivo calcium imaging of the aging and diseased brain
Authors: Gerhard Eichhoff
Marc Aurel Busche
Olga Garaschuk
Publication date: 01-03-2008
Publisher: Springer-Verlag
Published in: European Journal of Nuclear Medicine and Molecular Imaging / Issue Special Issue 1/2008
Print ISSN: 1619-7070
Electronic ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-007-0709-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

In vivo calcium imaging of the aging and diseased brain

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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