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Osteoporosis International

Published in:

01-02-2008 | Original Article

Improvement of Ca balance by Fructus Ligustri Lucidi extract in aged female rats

Authors: Y. Zhang, W.-P. Lai, P.-C. Leung, C.-T. Che, M.-S. Wong

Published in: Osteoporosis International | Issue 2/2008

Abstract

Summary

This study aimed to demonstrate and delineate the mechanism of action of Fructus Ligustri Lucidi (FLL) involved in improving Ca balance in aged female rats. FLL could enhance the apparent Ca absorption rate and reduce Ca excretion, via its actions on modulating the levels of calciotropic hormones and CaBPs expression.

Introduction

Fructus Ligustri Lucidi (FLL) is a herb classically used for the treatment of age-related symptoms in China. The present study aimed to determine if FLL could improve calcium balance in aged female rats and delineate its mechanism of action in vivo.

Methods

Aged Sprague-Dawley rats (11 months of age) were orally administered with ethanol extract of FLL or its vehicle and fed with diets containing different levels of Ca (low Ca diet, LCD, 0.1% Ca; medium Ca diet, MCD, 0.6% Ca; high Ca diet, HCD, 1.2% Ca) for 12 weeks. Serum, urine and feces were collected for biochemical markers and Ca balance determination. mRNA expressions of calcium binding proteins (CaBPs) were determined by real time RT-PCR. The effects of diets and herb were analyzed by both one-way and two-way ANOVA.

Results

FLL significantly reduced fecal Ca excretion and induced apparent Ca absorption rate in rats fed with MCD and HCD. FLL increased serum 1,25(OH)₂D₃ level and duodenal CaBP-9k mRNA expressions in all rats. Renal CaBP-28k mRNA expressions were induced in rats fed with MCD and HCD upon FLL treatment.

Conclusions

Our study demonstrated that FLL improved Ca balance in aged female rats by increasing serum 1,25 (OH)₂D₃ level and vitamin D-dependent CaBPs expression.

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Title: Improvement of Ca balance by Fructus Ligustri Lucidi extract in aged female rats
Authors: Y. Zhang
W.-P. Lai
P.-C. Leung
C.-T. Che
M.-S. Wong
Publication date: 01-02-2008
Publisher: Springer-Verlag
Published in: Osteoporosis International / Issue 2/2008
Print ISSN: 0937-941X
Electronic ISSN: 1433-2965
DOI: https://doi.org/10.1007/s00198-007-0442-9

At a glance: The STEP trials

Springer Medicine

Improvement of Ca balance by Fructus Ligustri Lucidi extract in aged female rats

Abstract

Summary

Introduction

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Summary

Introduction

Methods

Results

Conclusions

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