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01-01-2018 | Short Communication

Impact of several wearers on the persistence of DNA on clothes—a study with experimental scenarios

Authors: Micaela Poetsch, Manuel Pfeifer, Helen Konrad, Thomas Bajanowski, Janine Helmus

Published in: International Journal of Legal Medicine | Issue 1/2018

Abstract

The detection of DNA of a certain person on the inside of a piece of clothing involved in a crime scene is usually seen as confirmation that this person is the owner or bearer and therefore participated in this crime. However, besides the possibilities of secondary or even tertiary transfer of DNA, the accused often argues that he lent the garment to another person who by chance did not leave any DNA while committing the crime. Then, forensic genetic scientists have to answer the question how long DNA persists on an item used in daily routine and how long a piece of clothing must be worn to definitively leave detectable DNA behind. In an attempt to answer these questions, several scenarios with two or three individuals wearing the same sweatband for different time periods were set up. DNA left on the sweatbands was isolated, quantified, and then analyzed using the Powerplex® ESX17fast kit. The majority of samples displayed all alleles of both/all three wearers on the outside (67%) as well as on the inside (80%) of the sweatbands. In contrast, a single profile of the first wearer could only be found once among all 204 samples, a single profile of the second wearer in 7% of samples. Wearing the sweatband for only 10 min was enough to result in a complete profile of the second wearer in 79% of samples. So, it is highly unlikely to wear/use a piece of clothing for even a short period of time without leaving own DNA behind.

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Wang DY, Gopinath S, Lagace RE, Norona W, Hennessy LK, Short ML, Mulero JJ (2015) Developmental validation of the GlobalFiler® express PCR amplification kit: a 6-dye multiplex assay for the direct amplification of reference samples. Forensic Sci Int Genet 19:148–155. https://doi.org/10.1016/j.fsigen.2015.07.013 CrossRef

Ensenberger MG, Lenz KA, Matthies LK, Hadinoto GM, Schienman JE, Przech AJ, Morganti MW, Renstrom DT, Baker VM, Gawrys KM, Hoogendoorn M, Steffen CR, Martin P, alonso A, Olson HR, Sprecher DJ, Storts DR (2016) Developmental validation of the PowerPlex® fusion 6C system. Forensic Sci Int Genet 21:134–144, DOI: https://doi.org/10.1016/j.fsigen.2015.12.011

Schneider H, Sommerer T, Rand S, Wiegand P (2011) Hot flakes in cold cases. Int J Legal Med 125(4):543–548. https://doi.org/10.1007/s00414-011-0548-7 CrossRef

Elliott K, Hill DS, Lambert C, Burroughes TR, Gill P (2003) Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides. Forensic Sci Int 137(1):28–36. https://doi.org/10.1016/S0379-0738(03)00267-6 CrossRef

Di Martino D, Giuffre G, Staiti N, Simone A, Todaro P, Saravo L (2004) Laser microdissection and DNA typing of cells from single hair follicles. Forensic Sci Int 146(Suppl):S155–S157. https://doi.org/10.1016/j.forsciint.2004.09.047 CrossRef

Findlay I, Taylor A, Quirke P, Frazier R, Urquhart A (1997) DNA fingerprinting from single cells. Nature 389(6651):555–556. https://doi.org/10.1038/39225 CrossRef

Fonnelop AE, Egeland T, Gill P (2015) Secondary and subsequent DNA transfer during criminal investigation. Forensic Sci Int Genet 17:155–162. https://doi.org/10.1016/j.fsigen.2015.05.009 CrossRef

Szkuta B, Harvey ML, Ballantyne KN, van Oorschot RAH (2015) DNA transfer by examination tools—a risk for forensic casework? Forensic Sci Int Genet 16:246–254. https://doi.org/10.1016/j.fsigen.2015.02.004 CrossRef

Meakin G, Jamieson A (2013) DNA transfer: review and implications for casework. Forensic Sci Int Genet 7(4):434–443. https://doi.org/10.1016/j.fsigen.2013.03.013 CrossRef

10.

Goray M, Mitchell JR, van Oorschot RA (2012) Evaluation of multiple transfer of DNA using mock case scenarios. Leg Med (Tokyo) 14(1):40–46. https://doi.org/10.1016/j.legalmed.2011.09.006 CrossRef

11.

Helmus J, Zorell S, Bajanowski T, Poetsch M (2017) Persistence of DNA on clothes after exposure to water for different time periods—a study on bathtub, pond and river. Int J legal med. https://doi.org/10.1007/s00414-017-1695-2

12.

Lowe A, Murray C, Whitaker J, Tully G, Gill P (2002) The propensity of individuals to deposit DNA and secondary transfer of low level DNA from individuals to inert surfaces. Forensic Sci Int 129(1):25–34. https://doi.org/10.1016/S0379-0738(02)00207-4 CrossRef

13.

Phipps M, Petricevic S (2007) The tendency of individuals to transfer DNA to handled items. Forensic Sci Int 168(2-3):162–168. https://doi.org/10.1016/j.forsciint.2006.07.010 CrossRef

14.

Poetsch M, Bajanowski T, Kamphausen T (2013) Influence of an individual’s age on the amount and interpretability of DNA left on touched items. Int J Legal Med 127(6):1093–1096. https://doi.org/10.1007/s00414-013-0916-6 CrossRef

15.

Kamphausen T, Schadendorf D, von Wurmb-Schwark N, Bajanowski T, Poetsch M (2012) Good shedder or bad shedder—the influence of skin diseases on forensic DNA analysis from epithelial abrasions. Int J Legal Med 126:179–183, 1, DOI: https://doi.org/10.1007/s00414-011-0579-0

16.

Goray M, Eken E, Mitchell RJ, van Oorschot RA (2010) Secondary DNA transfer of biological substances under varying test conditions. Forensic Sci Int Genet 4(2):62–67. https://doi.org/10.1016/j.fsigen.2009.05.001 CrossRef

17.

Daly DJ, Murphy C, McDermott SD (2012) The transfer of touch DNA from hands to glass, fabric and wood. Forensic Sci Int Genet 6(1):41–46. https://doi.org/10.1016/j.fsigen.2010.12.016 CrossRef

18.

Raymond JJ, van Oorschot RA, Gunn PR, Walsh SJ, Roux C (2009) Trace evidence characteristics of DNA: a preliminary investigation of the persistence of DNA at crime scenes. Forensic Sci Int Genet 4(1):26–33. https://doi.org/10.1016/j.fsigen.2009.04.002 CrossRef

19.

Kamphausen T, Fandel SB, Gutmann JS, Bajanowski T, Poetsch M (2015) Everything clean? Transfer of DNA traces between textiles in the washtub. Int J Legal Med 129(4):709–714. https://doi.org/10.1007/s00414-015-1203-5 CrossRef

20.

Schwark T, Poetsch M, Preusse-Prange A, Kamphausen T, von Wurmb-Schwark N (2012) Phantoms in the mortuary-DNA transfer during autopsies. Forensic Sci Int 216(1-3):121–126. https://doi.org/10.1016/j.forsciint.2011.09.006 CrossRef

21.

Poetsch M, Konrad H, Helmus J, Bajanowski T, von Wurmb-Schwark N (2016) Does zero really mean nothing?—first experiences with the new PowerQuant™ system in comparison to established real time quantification kits. Int J Legal Med 130(4):935–940. https://doi.org/10.1007/s00414-016-1352-1 CrossRef

22.

Poetsch M, Bayer K, Ergin Z, Milbrath M, Schwark T, von Wurmb-Schwark N (2011) First experiences using the new Powerplex® ESX17 and ESI17 kits in casework analysis and allele frequencies from two different regions in Germany. Int J Legal Med 125(5):733–739. https://doi.org/10.1007/s00414-010-0480-2 CrossRef

23.

Helmus J, Bajanowski T, Poetsch M (2016) DNA transfer—a never ending story. A study on scenarios involving a second person as carrier. Int J Legal Med 130(1):121–125. https://doi.org/10.1007/s00414-015-1284-1 CrossRef

24.

Schneider PM, Fimmers R, Keil W, Molsberger G, Patzelt D, Pflug W, Rothamel T, Schmitter H, Schneider H, Brinkmann B (2009) The German stain commission: recommendations for the interpretation of mixed stains. Int J Legal Med 123(1):1–5. https://doi.org/10.1007/s00414-008-0244-4 CrossRef

25.

Meakin GE, Butcher EV, van Oorschot RAH, Morgan RM (2017) Trace DNA evidence dynamics: an investigation in to the deposition and persistence of directly—and indirectly—transferred DNA on regularly-used knifes. Forensic Sci Int Genet 29:38–47. https://doi.org/10.1016/j.fsigen.2017.03.016 CrossRef

26.

Taylor D, Biedermann A, Samie L, Pun K-M, Hicks T, Champod C (2017) Helping to distinguish primary from secondary transfer events for trace DNA. Forensic Sci Int Genet 28:155–177. https://doi.org/10.1016/j.fsigen.2017.02.008 CrossRef

27.

Fonnelǿp AE, Ramse M, Egeland T, Gill P (2017) The implications of shedder status and background DNA on direct and secondary transfer in an attack scenario. Forensic Sci Int Genet 29:48–60. https://doi.org/10.1016/j.fsigen.2017.03.019 CrossRef

Title: Impact of several wearers on the persistence of DNA on clothes—a study with experimental scenarios
Authors: Micaela Poetsch
Manuel Pfeifer
Helen Konrad
Thomas Bajanowski
Janine Helmus
Publication date: 01-01-2018
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Legal Medicine / Issue 1/2018
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-017-1742-z

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Impact of several wearers on the persistence of DNA on clothes—a study with experimental scenarios

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