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Clinical Oral Investigations

Published in:

01-06-2015 | Original Article

Impact of periodontitis on chemokines in smokers

Authors: O. Haytural, D. Yaman, E. C. Ural, A. Kantarci, Korkud Demirel

Published in: Clinical Oral Investigations | Issue 5/2015

Abstract

Objective

The aim of this study was to investigate the chemokine expression profiles in gingival crevicular fluid (GCF) and serum in patients with advanced chronic periodontitis and to assess the impact of smoking on local and systemic levels of chemokines.

Materials and methods

Thirty patients with chronic periodontitis (CP; 20 smokers and 10 non-smokers) and 20 periodontally healthy subjects (10 smokers and 10 non-smokers) were recruited. Clinical parameters included the plaque index (PI), gingival index (GI), and bleeding on probing (BOP). Macrophage inflammatory protein-1 alpha (MIP-1α), macrophage inflammatory protein-1 beta (MIP-1β), monocyte chemoattractant protein-1 (MCP-1), and regulated on activation normal T cell expressed and secreted chemokine (RANTES) were measured in gingival crevicular fluid (GCF) and serum using a multiplex immunoassay.

Results

MIP-1α levels were significantly lower (10.15 ± 1.48; p = 0.039) while MIP-1β levels were significantly higher (42.05 ± 8.21; p = 0.005) in sera from non-smoker patients with CP compared to non-smoker healthy subjects. MCP-1 concentration in sera was significantly higher in smoker periodontitis patients (8.89 ± 1.65) compared to non-smoker patients with periodontitis (8.14 ± 0.97; p = 0.004). MIP-1α and RANTES were significantly higher in GCF of the patients with CP (p = 0.001) while there were no statistically significant correlations between the GCF levels of these analytes and the smoking status.

Conclusion

Periodontal inflammation increases the chemokine concentrations in the GCF while smoking suppresses chemokine levels in serum suggesting that different local and systemic mechanisms are involved during the response to periodontitis in smokers.

Clinical relevance

Understanding the local and systemic chemokine responses in smokers will enable the development of biologically-based treatment methods for chronic periodontitis.

Periopaper, Oraflow Inc. New York, USA

Venoject Terumo Europe N.V.3001 Leuven, Belgium

Heraeus Labofuge 300, Kendro Laboratory Products, Osterrode, Germany

Luminex 100 MiraiBio, Alameda, CA, USA

Procarta® Cytokine Assay Kit, Panomics Inc. Fremont, USA

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Title: Impact of periodontitis on chemokines in smokers
Authors: O. Haytural
D. Yaman
E. C. Ural
A. Kantarci
Korkud Demirel
Publication date: 01-06-2015
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 5/2015
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-014-1314-2

At a glance: The STEP trials

Springer Medicine

Impact of periodontitis on chemokines in smokers

Abstract

Objective

Materials and methods

Results

Conclusion

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Materials and methods

Results

Conclusion

Clinical relevance

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