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Journal of Parasitic Diseases
Published in: Journal of Parasitic Diseases 2/2012

01-10-2012 | Original Article

Immunodiagnosis as an aid for early detection of Fasciola gigantica by glutathione S-transferase (GST)

Authors: S. Mandal, S. K. Mukhopadhayay, S. Ganguly, S. Jana

Published in: Journal of Parasitic Diseases | Issue 2/2012

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Abstract

In the present study, effort to isolate purified GST from Fasciola gigantica homogenate and its early detection for confirmation of infection has been successful. In this study, immunoprotection studies with GST and antibody titer post-infection has revealed GST to be an immunogenic protein with high sensitivity to detect anti-Fasciola antibody as early as two weeks post-infection in large ruminants.
Literature
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Richard LG (1995) Development and application of a dot-ELISA test for the detection of serum antibody to Fasciola hepatica antigen in lambs. Vet Parasitol 58:9–15CrossRef
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Wijfell GL, Sexton JL, Salvatore L, Pettitt JMM, Humphris DC, Panaccio M, Spithill TW (1992) Primary sequence heterogeneity and tissue expression of glutathione S-transferase of Fasciola hepatica. Exp Parasitol 74:87–99CrossRef
Yokananth S, Ghosh S, Gupta SC, Suresh MG, Saravanan D (2005) Characterization of specific and cross-reacting antigens of Fasciola gigantica by immunoblotting. Parasitol Res. doi:10.​1007/​S00436-005-1371-1
Metadata
Title
Immunodiagnosis as an aid for early detection of Fasciola gigantica by glutathione S-transferase (GST)
Authors
S. Mandal
S. K. Mukhopadhayay
S. Ganguly
S. Jana
Publication date
01-10-2012
Publisher
Springer-Verlag
Published in
Journal of Parasitic Diseases / Issue 2/2012
Print ISSN: 0971-7196
Electronic ISSN: 0975-0703
DOI
https://doi.org/10.1007/s12639-012-0106-3

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