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Archives of Virology
Published in: Archives of Virology 9/2011

01-09-2011 | Brief Report

Immunization with dendritic cells transfected in vivo with HIV-1 plasmid DNA induces HIV-1-specific immune responses

Authors: Maria Malm, Kai Krohn, Vesna Blazevic

Published in: Archives of Virology | Issue 9/2011

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Abstract

We evaluated the importance of dendritic cells (DCs) in the induction of the immune response after immunization of mice with DNA plasmid Auxo-GTU®-MultiHIV. First, GTU®-encoded protein was shown to be expressed by DCs of the draining lymph nodes (LNs) following intradermal (i.d.) immunization. Next, donor mice were immunized with the MultiHIV DNA plasmid, and DCs were enriched and further used to immunize naïve recipient mice. For the first time, the results show that i.d. immunization with Auxo-GTU®-MultiHIV transfects DCs in vivo, enabling them to present antigens and induce HIV-specific immune responses in recipient mice.
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Metadata
Title
Immunization with dendritic cells transfected in vivo with HIV-1 plasmid DNA induces HIV-1-specific immune responses
Authors
Maria Malm
Kai Krohn
Vesna Blazevic
Publication date
01-09-2011
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 9/2011
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-011-1003-2

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