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Virology Journal
Published in: Virology Journal 1/2018

Open Access 01-12-2018 | Research

Immunization with a suicidal DNA vaccine expressing the E glycoprotein protects ducklings against duck Tembusu virus

Authors: Jingyu Tang, Zhuangli Bi, Mingyang Ding, Dongdong Yin, Jie Zhu, Li Zhang, Qiuhong Miao, Yingqi Zhu, Guijun Wang, Guangqing Liu

Published in: Virology Journal | Issue 1/2018

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Abstract

Backgroud

Duck Tembusu virus (DTMUV), a pathogenic flavivirus, emerged in China since 2010 and causing huge economic loss in the Chinese poultry industry. Although several vaccines have been reported to control DTMUV disease, few effective vaccines are available and new outbreaks were continuously reported. Thus, it is urgently to develop a new effective vaccine for prevention of this disease.

Methods

In this study, a suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon and DTMUV E glycoprotein gene was constructed and the efficacy of this new vaccine was assessed according to humoral and cell-mediated immune responses as well as protection against the DTMUV challenge in ducklings.

Results

Our results showed that the recombinant SFV replicon highly expressed E glycoprotein in DEF cells. After intramuscular injection of this new DNA vaccine in ducklings, robust humoral and cellular immune responses were observed in all immunized ducklings. Moreover, all ducklings were protected against challenge with the virulent DTMUV AH-F10 strain.

Conclusions

In conclusion, we demonstrate that this suicidal DNA vaccine is a promising candidate facilitating the prevention of DTMUV infection.
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Metadata
Title
Immunization with a suicidal DNA vaccine expressing the E glycoprotein protects ducklings against duck Tembusu virus
Authors
Jingyu Tang
Zhuangli Bi
Mingyang Ding
Dongdong Yin
Jie Zhu
Li Zhang
Qiuhong Miao
Yingqi Zhu
Guijun Wang
Guangqing Liu
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2018
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-018-1053-0

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