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Virology Journal
Published in: Virology Journal 1/2016

Open Access 01-12-2016 | Research

Identification of mumps virus protein and lipid composition by mass spectrometry

Authors: Marija Brgles, Maximilian Bonta, Maja Šantak, Maja Jagušić, Dubravko Forčić, Beata Halassy, Günter Allmaier, Martina Marchetti-Deschmann

Published in: Virology Journal | Issue 1/2016

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Abstract

Background

Mumps virus is a negative-sense, single stranded RNA virus consisting of a ribonucleocapsid core enveloped by a lipid membrane derived from host cell, which causes mumps disease preventable by vaccination. Since virus lipid envelope and glycosylation pattern are not encoded by the virus but dependent on the host cell at least to some extent, the aim of this work was to analyse L-Zagreb (L-Zg) mumps virus lipids and proteins derived from two cell types; Vero and chicken embryo fibroblasts (CEF). Jeryl Lynn 5 (JL5) mumps strain lipids were also analysed.

Methods

Virus lipids were isolated by organic phase extraction and subjected to 2D-high performance thin layer chromatography followed by lipid extraction and identification by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Virus samples were also subjected to gel electrophoresis under denaturating conditions and protein bands were excised, in-gel trypsinized and identified by MS as well as tandem MS.

Results

Results showed that lipids of both mumps virus strains derived from Vero cells contained complex glycolipids with up to five monosaccharide units whereas the lipid pattern of mumps virus derived from CEF was less complex. Mumps virus was found to contain expected structural proteins with exception of fusion (F) protein which was not detected but on the other hand, V protein was detected. Most interesting finding related to the mumps proteins is the detection of several forms of nucleoprotein (NP), some of which appear to be C-terminally truncated.

Conclusions

Differences found in lipid and protein content of mumps virus demonstrated the importance of detailed biochemical characterization of mumps virus and the methodology described here could provide a means for a more comprehensive quality control in vaccine production.
Appendix
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Metadata
Title
Identification of mumps virus protein and lipid composition by mass spectrometry
Authors
Marija Brgles
Maximilian Bonta
Maja Šantak
Maja Jagušić
Dubravko Forčić
Beata Halassy
Günter Allmaier
Martina Marchetti-Deschmann
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2016
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-016-0463-0

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