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01-02-2009 | Research Paper

Identification of invasion specific splice variants of the cytoskeletal protein Mena present in mammary tumor cells during invasion in vivo

Authors: Sumanta Goswami, Ulrike Philippar, Daqian Sun, Antonia Patsialou, Jacob Avraham, Weigang Wang, Francesca Di Modugno, Paola Nistico, Frank B. Gertler, John S. Condeelis

Published in: Clinical & Experimental Metastasis | Issue 2/2009

Abstract

We have studied the gene expression pattern of invasive primary mammary tumor cells using a unique in vivo invasion assay that isolates the invasive tumor cells by chemotaxis. One of the genes upregulated in the invasive tumor cells is Mena, an actin binding protein involved in the regulation of cell motility. There are multiple known splice variants of Mena accounted for by four alternatively included exons, +, ++, +++ and 11a. Using the in vivo invasion assay in rats and mice with mammary tumors we observed that two isoforms of Mena, ++ and +++, are upregulated in the invasive tumor cells and one isoform, 11a, is downregulated. The Mena isoform switching pattern described here may provide a new biomarker for the presence of metastatic cancer cells and for prognosis.

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Title: Identification of invasion specific splice variants of the cytoskeletal protein Mena present in mammary tumor cells during invasion in vivo
Authors: Sumanta Goswami
Ulrike Philippar
Daqian Sun
Antonia Patsialou
Jacob Avraham
Weigang Wang
Francesca Di Modugno
Paola Nistico
Frank B. Gertler
John S. Condeelis
Publication date: 01-02-2009
Publisher: Springer Netherlands
Published in: Clinical & Experimental Metastasis / Issue 2/2009
Print ISSN: 0262-0898
Electronic ISSN: 1573-7276
DOI: https://doi.org/10.1007/s10585-008-9225-8

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