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01-06-2011 | Research Article

Identification of differential expressed transcripts in cervical cancer of Mexican patients

Authors: Leticia Santos, Ma. Fabiola León-Galván, Erika Nahomy Marino-Marmolejo, Ana Paulina Barba de la Rosa, Antonio De León Rodríguez, Roberto González-Amaro, Ramón Gerardo Guevara-González

Published in: Tumor Biology | Issue 3/2011

Abstract

The aim of this study was to identify the gene expression profile in biopsies of patients with cervical intraepithelial neoplasia (CIN) 1, CIN 2, CIN 3, and microinvasive cancer by suppression subtractive hybridization and Southern blotting. After analyzing 1,800 cDNA clones, we found 198 upregulated genes, 166 downregulated, and no significant change of gene expression in 86 clones (p = 0.005). These results were validated by Northern blot analysis (p = 0.0001) in the identification of 28 overexpressed and 7 downregulated transcripts. We observed a set of genes related to the Notch signaling pathway that may be involved in the transformation of cervical cells and in the development to malignancy. The differentially expressed genes may provide useful information about the molecular mechanisms involved in human cervical carcinoma and as diagnostic markers.

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Title: Identification of differential expressed transcripts in cervical cancer of Mexican patients
Authors: Leticia Santos
Ma. Fabiola León-Galván
Erika Nahomy Marino-Marmolejo
Ana Paulina Barba de la Rosa
Antonio De León Rodríguez
Roberto González-Amaro
Ramón Gerardo Guevara-González
Publication date: 01-06-2011
Publisher: Springer Netherlands
Published in: Tumor Biology / Issue 3/2011
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-010-0151-4

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