Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Calcified Tissue International
Published in: Calcified Tissue International 4/2006

01-10-2006 | Laboratory Investigations

Identification of a Promoter Element within the Zebrafish colXα1 Gene Responsive to Runx2 Isoforms Osf2/Cbfa1 and til-1 but not to pebp2αA2

Authors: B. Simões, N. Conceição, C. S. B. Viegas, J. P. Pinto, P. J. Gavaia, L. D. Hurst, R. N. Kelsh, M. L. Cancela

Published in: Calcified Tissue International | Issue 4/2006

Login to get access

Abstract

Type X collagen is a short chain collagen specifically expressed by hypertrophic chondrocytes during endochondral ossification. We report here the functional analysis of the zebrafish (Danio rerio) collagen Xα1 gene (colXα1) promoter with the identification of a region responsive to two isoforms of the runt domain transcription factor runx2. Furthermore, we provide evidence for the presence of dual promoter usage in zebrafish, a finding that should be important to further understanding of the regulation of its restricted tissue distribution and spatial-temporal expression during early development. The zebrafish colXα1 gene structure is comparable to that recently identified by comparative genomics in takifugu and shows homology with corresponding mammalian genes, indicating that its general architecture has been maintained throughout vertebrate evolution. Our data suggest that, as in mammals, runx2 plays a role in the development of the osteogenic lineage, supporting zebrafish as a model for studies of bone and cartilage development.
Appendix
Available only for authorised users
Literature
1.
Reichenberger E, Aigner T, von der Mark K, Sto H, Bertling W (1991) In situ hybridization studies on the expression of type X collagen in fetal human cartilage. Dev Biol 148:562–572PubMedCrossRef
2.
Beier F, Vornehm S, Poschl E, von der Mark K, Lammi MJ (1997) Localization of silencer and enhancer elements in the human type X collagen gene. J Cell Biochem 66:210–218PubMedCrossRef
3.
Riemer S, Gebhard S, Beier F, Poschl E, von der Mark K (2002) Role of c-fos in the regulation of type X collagen gene expression by PTH and PTHrP: localization of a PTH/PTHrP-responsive region in the human COL10A1 enhancer. J Cell Biochem 86:688–699PubMedCrossRef
4.
Eerola I, Elima K, Markkula M, Kananen K, Vuorio E (1996) Tissue distribution and phenotypic consequences of different type X collagen gene constructs in transgenic mice. Ann NY Acad Sci 785:248–250PubMedCrossRef
5.
Beier F, Lammi M, Bertling W, von der Mark K (1996) Transcriptional regulation of the human type X collagen gene expression. Ann NY Acad Sci 785:209–211PubMedCrossRef
6.
Jacenko O, LuValle PA, Olsen BR (1993) Spondylometaphyseal dysplasia in mice carrying a dominant negative mutation in a matrix protein specific for cartilage-to-bone transition. Nature 365:56–61PubMedCrossRef
7.
Dourado G, LuValle PA (1998) Proximal DNA elements mediate repressor activity conferred by the distal portion of the chicken collagen X promoter. J Cell Biochem 70:507–516PubMedCrossRef
8.
Magee C, Nurminskaya M, Faverman L, Galera P, Linsenmayer TF (2005) SP3/SP1 transcription activity regulates specific expression of collagen type X in hypertrophic chondrocytes. J Biol Chem 280:25331–25338PubMedCrossRef
9.
Ducy P, Zhang R, Geoffroy V, Ridall AL, Karsenty G (1997) Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell 89:747–754PubMedCrossRef
10.
Pinto J, Conceicao N, Viegas C, Leite R, Hurst L, Kelsh R, Cancela M (2005) Identification of a new pebp2alphaA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro. J Bone Miner Res 20:1440–1453PubMedCrossRef
11.
Jimenez MJG, Balbin M, Lopez JM, Alvarez J, Komori T, Lopez-Otin C (1999) Collagenase 3 is a target of Cbfa1, a transcription factor of the runt gene family involved in bone formation. Mol Cell Biol 19:4431–4442PubMed
12.
Zelzer E, Glotzer DJ, Hartmann C, Thomas D, Fukai N, Soker S, Olsen BR (2001) Tissue specific regulation of VEGF expression during bone development requires Cbfa1/Runx2. Mech Dev 106:97–106PubMedCrossRef
13.
Zheng Q, Zhou G, Morello R, Chen Y, Garcia-Rojas X, Lee B (2003) Type X collagen gene regulation by Runx2 contributes directly to its hypertrophic chondrocyte-specific expression in vivo. J Cell Biol 162:833–842PubMedCrossRef
14.
Sakakura C, Yamaguchi-Iwai Y, Satake M, Bae S, Takahashi A, Ogawa E, Hagiwara A, Takahashi T, Murakami A, Makino K, Nakagawa T, Kamada N, Ito Y (1994) Growth inhibition and induction of differentiation of t(8;21) acute myeloid leukemia cells by the DNA-binding domain of PEBP2 and the AML1/MTG8(ETO)-specific antisense oligonucleotide. Proc Natl Acad Sci USA 91:11723–11727PubMedCrossRef
15.
Harada H, Tagashira S, Fujiwara M, Ogawa S, Katsumata T, Yamaguchi A, Komori T, Nakatsuka M (1999) Cbfa1 isoforms exert functional differences in osteoblast differentiation. J Biol Chem 274:6972–6978PubMedCrossRef
16.
Banerjee C, Javed A, Choi J-Y, Green J, Rosen V, van Wijnen AJ, Stein JL, Lian JB, Stein GS (2001) Differential regulation of the two principal Runx2/Cbfa1 N-terminal isoforms in response to bone morphogenetic protein-2 during development of the osteoblast phenotype. Endocrinology 142:4026–4039PubMedCrossRef
17.
Avaron F, Hoffman L, Guay D, Akimenko MA (2006) Characterization of two new zebrafish members of the hedgehog family: atypical expression of a zebrafish indian hedgehog gene in skeletal elements of both endochondral and dermal origins. Dev Dyn 235:478–489PubMedCrossRef
18.
Padhi B, Joly L, Tellis P, Smith A, Nanjappa P, Chevrette M, Ekker M, Akimenko M (2004) Screen for genes differentially expressed during regeneration of the zebrafish caudal fin. Dev Dyn 231:527–541PubMedCrossRef
19.
Thisse C, Thisse B, Schilling TF, Postlethwait JH (1993) Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos. Development 119:1203–1215PubMed
20.
Sambrook J, Frisch E, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
21.
Cartharius K, Frech K, Grote K, Klocke B, Haltmeier M, Klingenhoff A, Frisch M, Bayerlein M, Werner T (2005) MatInspector and beyond: promoter analysis based on transcription factor binding sites. Bioinformatics 21:2933–2942PubMedCrossRef
22.
Edgar R (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797PubMedCrossRef
23.
Castresana J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17:540–552PubMed
24.
Kong R, Kwan K, Lau E, Thomas J, Boot-Handford R, Grant M, Cheah K (1993) Intron-exon structure, alternative use of promoter and expression of the mouse collagen X gene, Col10a-1. Eur J Biochem 213:99–111PubMedCrossRef
25.
Reichenberger E, Beier F, LuValle P, Olsen BR, von der Mark K, Bertling WM (1992) Genomic organization and full-length cDNA sequence of human collagen X. FEBS J 311:305–310CrossRef
26.
homas JT, Sweetman WA, Cresswell CJ, Wallis GA, Grant ME, Boot-Handford RP (1995) Sequence comparison of three mammalian type-X collagen promoters and preliminary functional analysis of the human promoter. Gene 160:291–296CrossRef
27.
Kamachi Y, Ogawa E, Asano M, Ishida S, Murakami, Satake M, Ito Y, Shigesada K (1990) Purification of a mouse nuclear factor that binds to both the A and B cores of the polyomavirus enhancer. J Virol 64:4808–4819PubMed
28.
Gascuel O (1997) BIONJ: an improved version of the NJ algorithm based on a simple model of sequence data. Mol Biol Evol 14:685–695PubMed
29.
Schmidt H, Strimmer K, Vingron M, von Haeseler A (2002) TREE-PUZZLE: maximum likelihood phylogenetic analysis using quartets and parallel computing. Bioinformatics 18:502–504PubMedCrossRef
30.
Campbell MR, Gress CJ, Appleman EH, Jacenko O (2004) Chicken collagen X regulatory sequences restrict transgene expression to hypertrophic cartilage in mice. Am J Pathol 164:487–499PubMed
31.
Ninomiya Y, Gordon M, van der Rest M, Schmid T, Linsenmeyer T, Olsen B (1986) The developmentally regulated type X collagene gene contains a long reading frame without introns. J Biol Chem 261:5041–5050PubMed
32.
Chan D, Jacenko O (1998) Phenotypic and biochemical consequences of collagen X mutations in mice and humans. Matrix Biol 17:169–184PubMedCrossRef
33.
Lu Valle P, Iwamoto M, Fanning P, Pacifici M, Olsen BR (1993) Multiple negative elements in a gene that codes for an extracellular matrix protein, collagen X, restrict expression to hypertrophic chondrocytes. J Cell Biol 121:1173–1179PubMedCrossRef
34.
Mizoguchi I, Takahashi I, Sasano Y, Kagayama M, Kuboki Y, Mitani H (1997) Localization of types I, II and X collagen and osteocalcin in intramembranous, endochondral and chondroid bone of rats. Anat Embryol (Berl) 196:291–297CrossRef
35.
Huysseune A, Van der heyden C, Sire J (1998) Early development of the zebrafish (Danio rerio) pharyngeal dentition (Teleostei, Cyprinidae). Anat Embryol (Berl) 198:289–305CrossRef
36.
Benjamin M, Ralphs JR, Eberewariye OS (1992) Cartilage and related tissues in the trunk and fins of teleosts. J Anat 181:113–118PubMed
37.
Woods A, James C, Underhill TM, Beier F (2004) Identification of the putative collagen X gene from the pufferfish Fugu rubripes. Gene 342:77–83PubMedCrossRef
38.
Bornstein P, McKay J (1988) The first intron of the alpha 1(I) collagen gene contains several transcriptional regulatory elements. J Biol Chem 263:1603–1606PubMed
39.
Horton W, Miyashita T, Kohno K, Hassell JR, Yamada Y (1987) Identification of a phenotype-specific enhancer in the first intron of the rat collagen II gene. Proc Natl Acad Sci USA 84:8864–8868PubMedCrossRef
40.
Long F, Sonenshein GE, Linsenmayer TF (1998) Multiple transcriptional elements in the avian type X collagen gene. Identification of sp1 family proteins as regulators for high level expression in hypertrophic chondrocytes. J Biol Chem 273:6542–6549PubMedCrossRef
41.
Long F, Linsenmayer TF (1995) Tissue-specific regulation of the type X collagen gene. J Biol Chem 270:31310–31314PubMedCrossRef
42.
Conceicao N, Silva AC, Fidalgo J, Belo JA, Cancela ML (2005) Identification of alternative promoter usage for the matrix Gla protein gene: evidence for differential expression during early development in Xenopus laevis. FEBS Lett 272:1501–1510
43.
Seth P, Mahajan VS, Chauhan SS (2003) Transcription of human cathepsin L mRNA species hCATL B from a novel alternative promoter in the first intron of its gene. Gene 321:83–91PubMedCrossRef
44.
Kahler RA, Westendorf JJ (2003) Lymphoid enhancer factor-1 and beta-catenin inhibit Runx2-dependent transcriptional activation of the osteocalcin promoter. J Biol Chem 278:11937–11944PubMedCrossRef
45.
Driever W, Solnica-Krezel L, Schier AF, Neuhauss SC, Malicki J, Stemple DL, Stainier DY, Zwartkruis F, Abdelilah S, Rangini Z, Belak J, Boggs C (1996) A genetic screen for mutations affecting embryogenesis in zebrafish. Development 123:37–46PubMed
Metadata
Title
Identification of a Promoter Element within the Zebrafish colXα1 Gene Responsive to Runx2 Isoforms Osf2/Cbfa1 and til-1 but not to pebp2αA2
Authors
B. Simões
N. Conceição
C. S. B. Viegas
J. P. Pinto
P. J. Gavaia
L. D. Hurst
R. N. Kelsh
M. L. Cancela
Publication date
01-10-2006
Publisher
Springer-Verlag
Published in
Calcified Tissue International / Issue 4/2006
Print ISSN: 0171-967X
Electronic ISSN: 1432-0827
DOI
https://doi.org/10.1007/s00223-006-0111-6

Other articles of this Issue 4/2006

Calcified Tissue International 4/2006 Go to the issue

Clinical Investigations

Application of a Triage Approach to Peripheral Bone Densitometry Reduces the Requirement for Central DXA but is not Cost Effective

OriginalPaper

Self-Reported Lifetime Physical Activity and Areal Bone Mineral Density in Healthy Postmenopausal Women: The Importance of Teenage Activity

OriginalPaper

Antiremodeling Agents Influence Osteoblast Activity Differently in Modeling and Remodeling Sites of Canine Rib

OriginalPaper

Calcaneus Ultrasonometry and Dual-Energy X-Ray Absorptiometry for the Evaluation of Vertebral Fracture Risk

OriginalPaper

Carotid Plaque Echogenicity and Risk of Nonvertebral Fractures in Women: A Longitudinal Population-Based Study

OriginalPaper

Daily Treatment of Aged Ovariectomized Rats with Human Parathyroid Hormone (1-84) for 12 Months Reverses Bone Loss and Enhances Trabecular and Cortical Bone Strength
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Heart Failure Video

Year in Review: Heart failure and cardiomyopathies

Watch now

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits