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Archives of Virology
Published in: Archives of Virology 10/2016

01-10-2016 | Brief Report

Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies

Authors: Xiaofei Li, Liting Qin, Haibo Zhu, Yingjun Sun, Xuezhi Cui, Yadong Gao, Xiaole Qi, Yongqiang Wang, Honglei Gao, Yulong Gao, Xiaomei Wang

Published in: Archives of Virology | Issue 10/2016

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Abstract

Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can induce various clinical tumors. The capsid protein P27 is the group-specific antigen of ALV and has many viral antigen sites that are easy to detect. In this study, we produced a monoclonal antibody (mAb), 3A9, that is specific for the P27 protein. A series of partially overlapping peptides were screened to define 181PPSAR185 as the minimal linear epitope recognized by mAb 3A9. The identified epitope could be recognized by chicken anti-ALV and mouse anti-ALV P27 sera. The epitope was highly conserved among a number of ALV-A, ALV-B and ALV-J strains. MAb 3A9 might be a valuable tool for the development of new immunodiagnostic approaches for ALV, and the defined linear epitope might help further our understanding of the antigenic structure of the P27 protein.
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Metadata
Title
Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies
Authors
Xiaofei Li
Liting Qin
Haibo Zhu
Yingjun Sun
Xuezhi Cui
Yadong Gao
Xiaole Qi
Yongqiang Wang
Honglei Gao
Yulong Gao
Xiaomei Wang
Publication date
01-10-2016
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 10/2016
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-016-2971-z

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