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Published in: Archives of Virology 12/2015

01-12-2015 | Brief Report

Identification and genetic characterization of two porcine astroviruses from domestic piglets in China

Authors: Jin-song Li, Mao-zhong Li, Li-shu Zheng, Na Liu, Dan-di Li, Zhao-jun Duan

Published in: Archives of Virology | Issue 12/2015

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Abstract

Porcine astrovirus are divided into five genotypes. In this study, we identified two porcine astroviruses (AstV-LL-1 and AstV-LL-2) by using sequence-independent single-primer amplification (SISPA) on faecal specimens of healthy domestic piglets younger than 15 days. The detection rate for both was 2.82 % (14/497). AstV-LLs were then sequenced and characterised. Phylogenetic analysis revealed that they have the characteristics of porcine astrovirus (PastV) 2 and 5 and have some unique genetic features. Our findings show that the two astroviruses are novel lineages of PAstV2 and 5. The findings may be helpful in evaluating the ecology and evolution of astroviruses in pigs.
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Literature
2.
go back to reference Brnic D, Prpic J, Keros T, Roic B, Staresina V, Jemersic L (2013) Porcine astrovirus viremia and high genetic variability in pigs on large holdings in Croatia. Infect Genet Evol 14:258–264CrossRefPubMed Brnic D, Prpic J, Keros T, Roic B, Staresina V, Jemersic L (2013) Porcine astrovirus viremia and high genetic variability in pigs on large holdings in Croatia. Infect Genet Evol 14:258–264CrossRefPubMed
3.
go back to reference Cheng WX, Li JS, Huang CP, Yao DP, Liu N, Cui SX, Jin Y, Duan ZJ (2010) Identification and nearly full-length genome characterization of novel porcine bocaviruses. PLoS ONE 5:e13583PubMedCentralCrossRefPubMed Cheng WX, Li JS, Huang CP, Yao DP, Liu N, Cui SX, Jin Y, Duan ZJ (2010) Identification and nearly full-length genome characterization of novel porcine bocaviruses. PLoS ONE 5:e13583PubMedCentralCrossRefPubMed
4.
go back to reference Dryden KA, Tihova M, Nowotny N, Matsui SM, Mendez E, Yeager M (2012) Immature and mature human astrovirus: structure, conformational changes, and similarities to hepatitis E virus. J Mol Biol 422:650–658CrossRefPubMed Dryden KA, Tihova M, Nowotny N, Matsui SM, Mendez E, Yeager M (2012) Immature and mature human astrovirus: structure, conformational changes, and similarities to hepatitis E virus. J Mol Biol 422:650–658CrossRefPubMed
5.
go back to reference Enserink M (2000) Emerging diseases. Malaysian researchers trace Nipah virus outbreak to bats. Science 289:518–519CrossRefPubMed Enserink M (2000) Emerging diseases. Malaysian researchers trace Nipah virus outbreak to bats. Science 289:518–519CrossRefPubMed
6.
go back to reference Gough RE, Collins MS, Borland E, Keymer LF (1984) Astrovirus-like particles associated with hepatitis in ducklings. Vet Rec 114:279CrossRefPubMed Gough RE, Collins MS, Borland E, Keymer LF (1984) Astrovirus-like particles associated with hepatitis in ducklings. Vet Rec 114:279CrossRefPubMed
7.
go back to reference Jonassen CM, Jonassen TO, Saif YM, Snodgrass DR, Ushijima H, Shimizu M, Grinde B (2001) Comparison of capsid sequences from human and animal astroviruses. J Gen Virol 82:1061–1067CrossRefPubMed Jonassen CM, Jonassen TO, Saif YM, Snodgrass DR, Ushijima H, Shimizu M, Grinde B (2001) Comparison of capsid sequences from human and animal astroviruses. J Gen Virol 82:1061–1067CrossRefPubMed
8.
go back to reference Laurin MA, Dastor M, L’Homme Y (2011) Detection and genetic characterization of a novel pig astrovirus: relationship to other astroviruses. Arch Virol 156:2095–2099CrossRefPubMed Laurin MA, Dastor M, L’Homme Y (2011) Detection and genetic characterization of a novel pig astrovirus: relationship to other astroviruses. Arch Virol 156:2095–2099CrossRefPubMed
9.
go back to reference Luo Z, Roi S, Dastor M, Gallice E, Laurin MA, L’Homme Y (2011) Multiple novel and prevalent astroviruses in pigs. Vet Microbiol 149:316–323CrossRefPubMed Luo Z, Roi S, Dastor M, Gallice E, Laurin MA, L’Homme Y (2011) Multiple novel and prevalent astroviruses in pigs. Vet Microbiol 149:316–323CrossRefPubMed
10.
go back to reference Mendez-Toss M, Romero-Guido P, Munguia ME, Mendez E, Arias CF (2000) Molecular analysis of a serotype 8 human astrovirus genome. J Gen Virol 81:2891–2897CrossRefPubMed Mendez-Toss M, Romero-Guido P, Munguia ME, Mendez E, Arias CF (2000) Molecular analysis of a serotype 8 human astrovirus genome. J Gen Virol 81:2891–2897CrossRefPubMed
11.
go back to reference Monceyron C, Grinde B, Jonassen TO (1997) Molecular characterisation of the 3’-end of the astrovirus genome. Arch Virol 142:699–706CrossRefPubMed Monceyron C, Grinde B, Jonassen TO (1997) Molecular characterisation of the 3’-end of the astrovirus genome. Arch Virol 142:699–706CrossRefPubMed
12.
go back to reference Reuter G, Pankovics P, Boros A (2011) Identification of a novel astrovirus in a domestic pig in Hungary. Arch Virol 156:125–128CrossRefPubMed Reuter G, Pankovics P, Boros A (2011) Identification of a novel astrovirus in a domestic pig in Hungary. Arch Virol 156:125–128CrossRefPubMed
14.
go back to reference Wang QH, Kakizawa J, Wen LY, Shimizu M, Nishio O, Fang ZY, Ushijima H (2001) Genetic analysis of the capsid region of astroviruses. J Med Virol 64:245–255CrossRefPubMed Wang QH, Kakizawa J, Wen LY, Shimizu M, Nishio O, Fang ZY, Ushijima H (2001) Genetic analysis of the capsid region of astroviruses. J Med Virol 64:245–255CrossRefPubMed
15.
16.
go back to reference Xiao CT, Gimenez-Lirola LG, Gerber PF, Jiang YH, Halbur PG, Opriessnig T (2013) Identification and characterization of novel porcine astroviruses (PAstVs) with high prevalence and frequent co-infection of individual pigs with multiple PAstV types. J Gen Virol 94:570–582CrossRefPubMed Xiao CT, Gimenez-Lirola LG, Gerber PF, Jiang YH, Halbur PG, Opriessnig T (2013) Identification and characterization of novel porcine astroviruses (PAstVs) with high prevalence and frequent co-infection of individual pigs with multiple PAstV types. J Gen Virol 94:570–582CrossRefPubMed
17.
go back to reference Yu H, Jing H, Chen Z, Zheng H, Zhu X, Wang H, Wang S, Liu L, Zu R, Luo L, Xiang N, Liu H, Liu X, Shu Y, Lee SS, Chuang SK, Wang Y, Xu J, Yang W (2006) Human Streptococcus suis outbreak, Sichuan, China. Emerg Infect Dis 12:914–920PubMedCentralCrossRefPubMed Yu H, Jing H, Chen Z, Zheng H, Zhu X, Wang H, Wang S, Liu L, Zu R, Luo L, Xiang N, Liu H, Liu X, Shu Y, Lee SS, Chuang SK, Wang Y, Xu J, Yang W (2006) Human Streptococcus suis outbreak, Sichuan, China. Emerg Infect Dis 12:914–920PubMedCentralCrossRefPubMed
Metadata
Title
Identification and genetic characterization of two porcine astroviruses from domestic piglets in China
Authors
Jin-song Li
Mao-zhong Li
Li-shu Zheng
Na Liu
Dan-di Li
Zhao-jun Duan
Publication date
01-12-2015
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 12/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-015-2569-x

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