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Diagnostic Pathology
Published in: Diagnostic Pathology 1/2013

Open Access 01-12-2013 | Research

Hypermethylation of EDNRB promoter contributes to the risk of colorectal cancer

Authors: Cheng Chen, Lingyan Wang, Qi Liao, Yi Huang, Huadan Ye, Fei Chen, Leiting Xu, Meng Ye, Shiwei Duan

Published in: Diagnostic Pathology | Issue 1/2013

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Abstract

Objective

Colorectal cancer (CRC) is one of the most common digestive malignancies in the world. EDNRB is a new candidate tumor suppressor gene which is often down-regulated or even silenced by promoter hypermethylation in various human cancers. However, the function of EDNRB gene in CRC remains unknown. In this study, we examined the expression and DNA methylation of EDNRB in CRC tissues.

Methods

A total of 42 paired CRC and adjacent normal tissue samples were used to determine mRNA levels and DNA methylation status of EDNRB gene by qRT-PCR and methylation-specific PCR (MSP), respectively.

Results

Our study showed that EDNRB promoter hypermethylation was more frequently in CRC tissues than in the normal tissues (92.86 versus 59.52, p = 0.001). Consequently, significantly lower level of EDNRB mRNA was found in CRC tumor samples than in normal samples (0.31 ± 0.91 versus 0.70 ± 1.18, p = 0.032).

Conclusions

Our results suggested that EDNRB promoter hypermethylation might downregulate its gene expression in CRC, and thus played an important role in the development of CRC.

The virtual slide

The virtual slides for this article can be found here: http://​www.​diagnosticpathol​ogy.​diagnomx.​eu/​vs/​7420980471113303​
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Metadata
Title
Hypermethylation of EDNRB promoter contributes to the risk of colorectal cancer
Authors
Cheng Chen
Lingyan Wang
Qi Liao
Yi Huang
Huadan Ye
Fei Chen
Leiting Xu
Meng Ye
Shiwei Duan
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Diagnostic Pathology / Issue 1/2013
Electronic ISSN: 1746-1596
DOI
https://doi.org/10.1186/1746-1596-8-199

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