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Intensive Care Medicine

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01-06-2008 | Experimental

Hyperbaric oxygenation reduces overexpression of c-Fos and oxidative stress in the brain stem of experimental endotoxemic rats

Authors: Hui-Ching Lin, Fang-Jung Wan

Published in: Intensive Care Medicine | Issue 6/2008

Abstract

Objective

Septic encephalopathy is associated with an increased mortality rate in septic patients. We have previously shown that a peripheral lipopolysaccharide (LPS) injection induces neuronal activation in the brain-stem nuclei of rats. Nitric oxide (NO) and superoxide are involved in LPS-induced brain damage. Hyperbaric oxygenation (HBO) provides protective effects against systemic oxidative stress and mortality in animals with septic shock. We examined the effects of HBO on neuronal activation and oxidative stress in the brain-stem nuclei of LPS-treated rats.

Design and interventions

Wistar rats were randomly distributed into six groups for the following treatments:(a) normal saline injection (NS); (b) HBO; (c) LPS; (d) LPS–HBO; (e) LPS–aminoguanidine (AG, an inhibitor of inducible nitric oxide synthase); or (f) hydralazine (HYD, a direct vasodilator). The HYD induces prolonged hypotension and was used as a comparison for LPS stimulation. The AG was used as a comparison for HBO treatment. Two HBO sessions were administered, 1 and 4 h after LPS.

Results

HBO and AG significantly reversed the overproduction of c-Fos induced by LPS in the brain stems of rats, with greater reversal in the nucleus tractus solitarii (NTS) by HBO. Although AG did not reduce the superoxide level, HBO significantly abolished superoxide production and NADPH diaphorase expression in the brain stems of LPS-treated rats. The HYD induced much lower c-Fos expression in the brain-stem nuclei than that in LPS-treated animals and caused no significant increase in NADPH diaphorase expression or superoxide formation.

Conclusion

HBO protects against endotoxin-related neuronal activation and oxidative stress in the brain-stem nuclei of rats.

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Title: Hyperbaric oxygenation reduces overexpression of c-Fos and oxidative stress in the brain stem of experimental endotoxemic rats
Authors: Hui-Ching Lin
Fang-Jung Wan
Publication date: 01-06-2008
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 6/2008
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-007-0986-3

At a glance: The STEP trials

Springer Medicine

Hyperbaric oxygenation reduces overexpression of c-Fos and oxidative stress in the brain stem of experimental endotoxemic rats

Abstract

Objective

Design and interventions

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Design and interventions

Results

Conclusion

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