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Fibrogenesis & Tissue Repair
Published in: Fibrogenesis & Tissue Repair 1/2011

Open Access 01-12-2011 | Research

Hydroxyproline-containing collagen analogs trigger the release and activation of collagen-sequestered proMMP-2 by competition with prodomain-derived peptide P33-42

Authors: Martin Ruehl, Marion Muche, Christian Freise, Ulrike Erben, Ulf Neumann, Detlef Schuppan, Yury Popov, Walburga Dieterich, Martin Zeitz, Richard W Farndale, Rajan Somasundaram

Published in: Fibrogenesis & Tissue Repair | Issue 1/2011

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Abstract

Background

Fibrolytic and profibrotic activities of the matrix metalloproteinases (MMPs)-2 and -9 play a central role in liver fibrosis. Since binding to the extracellular matrix influences the activity of both gelatinases, here the role of fibrillar collagens as the most abundant matrix components in fibrotic tissue was investigated.

Results

In situ zymography and immunohistology showed association of enzymatically inactive prodomain-containing proMMP-2 and proMMP-9 but not of their activated forms to fibrillar collagen structures, which are not substrates of these gelatinases. In solid-phase binding studies with human collagens and collagen fragments, up to 45% of [125I]-labeled proMMP-2 and proMMP-9 but not of active (act)MMP-2 and actMMP-9 were retained by natural collagenous molecules and by synthetic analogs containing repeated Gly-Pro-Hyp triplets (GPO). Surface plasmon resonance yielded binding constants for the interaction of collagen type I (CI) with proMMP-2 and proMMP-9 in a nanomolar range. Values for actMMP-2 and actMMP-9 were 30-40 times higher. Tenfold molar excesses of (GPO)10 reduced the interaction of CI with pro- and actMMP-2 by 22- or 380-fold and resulted in prodomain release accompanied by high enzymatic activation and activity. Pointing to gelatine substrate displacement, higher (GPO)10 concentrations blocked the enzymatic activity. The MMP-2 prodomain-derived collagen-binding domain peptide (P33-42) binds to the collagen-binding domain of MMP-2, thereby preserving enzymatic inactivity. Synthetic P33-42 peptide competed with proMMP-2 binding to CI and prevented (GPO)10-mediated proMMP-2 activation. In contrast to (GPO)10, P33-42 did not activate proMMP-2, making triple helical and hydroxyproline-containing (GPO)10 unique in modulating gelatinase availability and activity.

Conclusions

These findings suggest novel strategies using collagen analogs for the resolution of liver fibrosis via fibrotic matrix-sequestered gelatinases.
Appendix
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Metadata
Title
Hydroxyproline-containing collagen analogs trigger the release and activation of collagen-sequestered proMMP-2 by competition with prodomain-derived peptide P33-42
Authors
Martin Ruehl
Marion Muche
Christian Freise
Ulrike Erben
Ulf Neumann
Detlef Schuppan
Yury Popov
Walburga Dieterich
Martin Zeitz
Richard W Farndale
Rajan Somasundaram
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Fibrogenesis & Tissue Repair / Issue 1/2011
Electronic ISSN: 1755-1536
DOI
https://doi.org/10.1186/1755-1536-4-1

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