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Virology Journal
Published in: Virology Journal 1/2013

Open Access 01-12-2013 | Research

Human papillomavirus genotyping and integration in ovarian cancer Saudi patients

Authors: Othman A Al-Shabanah, Mohamed M Hafez, Zeinab K Hassan, Mohamed M Sayed-Ahmed, Waleed N Abozeed, Salem S Al-Rejaie, Abdulmalik A Alsheikh

Published in: Virology Journal | Issue 1/2013

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Abstract

Background

Human papillomavirus (HPV) is associated with different malignancies but its role in the pathogenesis of ovarian cancer is controversial. This study investigated the prevalence, genotyping and physical state of HPV in ovarian cancer Saudi patients.

Methods

Hundred formalin fixed paraffin embedded (FFPE) ovarian carcinoma tissues and their normal adjacent tissues (NAT) were included in the study. HPV was detected by nested polymerase chain reaction (PCR) using degenerated HPVL1 consensus primer pairs MY09/MY11 and GP5+/GP6 + to amplify a broad spectrum of HPV genotypes in a single reaction. The HPV positive samples were further genotyped using DNA sequencing. The physical state of the virus was identified using Amplification of Papillomavirus Oncogene Transcripts (APOT) assay in the samples positive for HPV16 and/or HPV18.

Results

High percentage of HPV (42%) was observed in ovarian carcinoma compared to 8% in the NAT. The high-risk HPV types 16, 18 and 45 were highly associated with the advanced stages of tumor, while low-risk types 6 and 11 were present in NAT. In malignant tissues, HPV-16 was the most predominant genotype followed by HPV-18 and -45. The percentage of viral integration into the host genome was significantly high (61.1%) compared to 38.9% episomal in HPV positive tumors tissues. In HPV18 genotype the percentage of viral integration was 54.5% compared to 45.5% episomal.

Conclusion

The high risk HPV genotypes in ovarian cancer may indicate its role in ovarian carcinogenesis. The HPV vaccination is highly recommended to reduce this type of cancer.
Appendix
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Metadata
Title
Human papillomavirus genotyping and integration in ovarian cancer Saudi patients
Authors
Othman A Al-Shabanah
Mohamed M Hafez
Zeinab K Hassan
Mohamed M Sayed-Ahmed
Waleed N Abozeed
Salem S Al-Rejaie
Abdulmalik A Alsheikh
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2013
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-10-343

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