Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Virology Journal
Published in: Virology Journal 1/2013

Open Access 01-12-2013 | Short report

Development and characterization of the replicon system of Japanese encephalitis live vaccine virus SA14-14-2

Authors: Shi-Hua Li, Xiao-Feng Li, Hui Zhao, Yong-Qiang Deng, Xue-Dong Yu, Shun-Ya Zhu, Tao Jiang, Qing Ye, E-De Qin, Cheng-Feng Qin

Published in: Virology Journal | Issue 1/2013

Login to get access

Abstract

Background

Viral self-replicating sub-genomic replicons represent a powerful tool for studying viral genome replication, antiviral screening and chimeric vaccine development. Many kinds of flavivirus replicons have been developed with broad applications.

Findings

The replicon system of JEV live vaccine strain SA14-14-2 was successfully developed in this study. Two kinds of replicons that express enhanced green fluorescent protein (EGFP) and Renilla luciferase (R.luc) were constructed under the control of SP6 promoter, respectively. Robust EGFP and R.luc signals could be detected in the replicon-transfected BHK-21 cells. Furthermore, the potential effects of selected amino acids in the C-terminal of envelope protein on replication were characterized using the replicon system.

Conclusions

Our results provide a useful platform not only for the study of JEV replication, but also for antiviral screening and chimeric vaccine development.
Appendix
Available only for authorised users
Literature
1.
Kumar R, Tripathi P, Singh S, Bannerji G: Clinical features in children hospitalized during the 2005 epidemic of Japanese encephalitis in uttar pradesh, India. Clin Infect Dis 2006, 43: 123-131. 10.1086/505121PubMedCrossRef
2.
Murgod UA, Muthane UB, Ravi V, Radhesh S, Desai A: Persistent movement disorders following Japanese encephalitis. Neurology 2001, 57: 2313-2315. 10.1212/WNL.57.12.2313PubMedCrossRef
3.
Misra UK, Kalita J: Overview: Japanese encephalitis. Prog Neurobiol 2010, 91: 108-120. 10.1016/j.pneurobio.2010.01.008PubMedCrossRef
4.
Erlanger TE, Weiss S, Keiser J, Utzinger J, Wiedenmayer K: Past, present, and future of Japanese encephalitis. Emerg Infect Dis 2009, 15: 1-7. 10.3201/eid1501.080311PubMedPubMedCentralCrossRef
5.
Endy TP, Nisalak A: Japanese encephalitis virus: ecology and epidemiology. Curr Top Microbiol Immunol 2002, 267: 11-48. 10.1007/978-3-642-59403-8_2PubMed
6.
Mackenzie JS, Johansen CA, Ritchie SA, van den Hurk AF, Hall RA: Japanese encephalitis as an emerging virus: the emergence and spread of Japanese encephalitis virus in Australasia. Curr Top Microbiol Immunol 2002, 267: 49-73. 10.1007/978-3-642-59403-8_3PubMed
7.
Yun SI, Choi YJ, Song BH, Lee YM: 3′ cis-acting elements that contribute to the competence and efficiency of Japanese encephalitis virus genome replication: functional importance of sequence duplications, deletions, and substitutions. J Virol 2009, 83: 7909-7930. 10.1128/JVI.02541-08PubMedPubMedCentralCrossRef
8.
Oya A, Kurane I: Japanese encephalitis for a reference to international travelers. J Travel Med 2007, 14: 259-268. 10.1111/j.1708-8305.2007.00134.xPubMedCrossRef
9.
Halstead SB, Thomas SJ: New Japanese encephalitis vaccines: alternatives to production in mouse brain. Expert Rev Vaccines 2011, 10: 355-364. 10.1586/erv.11.7PubMedCrossRef
10.
Fischer M, Lindsey N, Staples JE, Hills S: Japanese encephalitis vaccines: recommendations of the advisory committee on immunization practices (ACIP). MMWR Recomm Rep 2010, 59: 1-27.
11.
Yun SI, Choi YJ, Yu XF, Song JY, Shin YH, Ju YR, Kim SY, Lee YM: Engineering the Japanese encephalitis virus RNA genome for the expression of foreign genes of various sizes: implications for packaging capacity and RNA replication efficiency. J Neurovirol 2007, 13: 522-535. 10.1080/13550280701684651PubMedCrossRef
12.
Khromykh AA, Westaway EG: Subgenomic replicons of the flavivirus Kunjin: construction and applications. J Virol 1997, 71: 1497-1505.PubMedPubMedCentral
13.
Gehrke R, Ecker M, Aberle SW, Allison SL, Heinz FX, Mandl CW: Incorporation of tick-borne encephalitis virus replicons into virus-like particles by a packaging cell line. J Virol 2003, 77: 8924-8933. 10.1128/JVI.77.16.8924-8933.2003PubMedPubMedCentralCrossRef
14.
Mosimann AL, de Borba L, Bordignon J, Mason PW, dos Santos CN: Construction and characterization of a stable subgenomic replicon system of a Brazilian dengue virus type 3 strain (BR DEN3 290–02). J Virol Methods 2010, 163: 147-152. 10.1016/j.jviromet.2009.09.004PubMedCrossRef
15.
Suzuki R, de Borba L, Santos CN Dd, Mason PW: Construction of an infectious cDNA clone for a Brazilian prototype strain of dengue virus type 1: characterization of a temperature-sensitive mutation in NS1. Virology 2007, 362: 374-383. 10.1016/j.virol.2006.11.026PubMedPubMedCentralCrossRef
16.
Ng CY, Gu F, Phong WY, Chen YL, Lim SP, Davidson A, Vasudevan SG: Construction and characterization of a stable subgenomic dengue virus type 2 replicon system for antiviral compound and siRNA testing. Antiviral Res 2007, 76: 222-231. 10.1016/j.antiviral.2007.06.007PubMedCrossRef
17.
Pang X, Zhang M, Dayton AI: Development of Dengue virus type 2 replicons capable of prolonged expression in host cells. BMC Microbiol 2001, 1: 18. 10.1186/1471-2180-1-18PubMedPubMedCentralCrossRef
18.
Corver J, Lenches E, Smith K, Robison RA, Sando T, Strauss EG, Strauss JH: Fine mapping of a cis-acting sequence element in yellow fever virus RNA that is required for RNA replication and cyclization. J Virol 2003, 77: 2265-2270. 10.1128/JVI.77.3.2265-2270.2003PubMedPubMedCentralCrossRef
19.
Jones CT, Patkar CG, Kuhn RJ: Construction and applications of yellow fever virus replicons. Virology 2005, 331: 247-259. 10.1016/j.virol.2004.10.034PubMedCrossRef
20.
Rossi SL, Zhao Q, O’Donnell VK, Mason PW: Adaptation of West Nile virus replicons to cells in culture and use of replicon-bearing cells to probe antiviral action. Virology 2005, 331: 457-470. 10.1016/j.virol.2004.10.046PubMedCrossRef
21.
Shi PY, Tilgner M, Lo MK: Construction and characterization of subgenomic replicons of New York strain of west nile virus. Virology 2002, 296: 219-233. 10.1006/viro.2002.1453PubMedCrossRef
22.
Yamshchikov VF, Wengler G, Perelygin AA, Brinton MA, Compans RW: An infectious clone of the west nile flavivirus. Virology 2001, 281: 294-304. 10.1006/viro.2000.0795PubMedCrossRef
23.
Moritoh K, Maeda A, Nishino T, Sasaki N, Agui T: Development and application of west nile virus subgenomic replicon RNA expressing secreted alkaline phosphatase. J Vet Med Sci 2011, 73: 683-686. 10.1292/jvms.10-0495PubMedCrossRef
24.
Mishin VP, Cominelli F, Yamshchikov VF: A ‘minimal’ approach in design of flavivirus infectious DNA. Virus Res 2001, 81: 113-123. 10.1016/S0168-1702(01)00371-9PubMedCrossRef
25.
Sumiyoshi H, Hoke CH, Trent DW: Infectious Japanese encephalitis virus RNA can be synthesized from in vitro -ligated cDNA templates. J Virol 1992, 66: 5425-5431.PubMedPubMedCentral
26.
Sumiyoshi H, Tignor GH, Shope RE: Characterization of a highly attenuated Japanese encephalitis virus generated from molecularly cloned cDNA. J Infect Dis 1995, 171: 1144-1151. 10.1093/infdis/171.5.1144PubMedCrossRef
27.
Zhang F, Huang Q, Ma W, Jiang S, Fan Y, Zhang H: Amplification and cloning of the full-length genome of Japanese encephalitis virus by a novel long RT-PCR protocol in a cosmid vector. J Virol Methods 2001, 96: 171-182. 10.1016/S0166-0934(01)00331-7PubMedCrossRef
28.
Yoshii K, Holbrook MR: Sub-genomic replicon and virus-like particles of Omsk hemorrhagic fever virus. Arch Virol 2009, 154: 573-580. 10.1007/s00705-009-0345-5PubMedCrossRef
29.
Alvarez DE, Lodeiro MF, Luduena SJ, Pietrasanta LI, Gamarnik AV: Long-range RNA-RNA interactions circularize the dengue virus genome. J Virol 2005, 79: 6631-6643. 10.1128/JVI.79.11.6631-6643.2005PubMedPubMedCentralCrossRef
30.
Bredenbeek PJ, Kooi EA, Lindenbach B, Huijkman N, Rice CM, Spaan WJ: A stable full-length yellow fever virus cDNA clone and the role of conserved RNA elements in flavivirus replication. J Gen Virol 2003, 84: 1261-1268. 10.1099/vir.0.18860-0PubMedCrossRef
31.
Khromykh AA, Meka H, Guyatt KJ, Westaway EG: Essential role of cyclization sequences in flavivirus RNA replication. J Virol 2001, 75: 6719-6728. 10.1128/JVI.75.14.6719-6728.2001PubMedPubMedCentralCrossRef
32.
Cao F, Li XF, Yu XD, Deng YQ, Jiang T, Zhu QY, Qin ED, Qin CF: A DNA-based west nile virus replicon elicits humoral and cellular immune responses in mice. J Virol Methods 2011, 178: 87-93. 10.1016/j.jviromet.2011.08.018PubMedCrossRef
33.
Patkar CG, Larsen M, Owston M, Smith JL, Kuhn RJ: Identification of inhibitors of yellow fever virus replication using a replicon-based high-throughput assay. Antimicrob Agents Chemother 2009, 53: 4103-4114. 10.1128/AAC.00074-09PubMedPubMedCentralCrossRef
34.
Ye Q, Li XF, Zhao H, Li SH, Deng YQ, Cao RY, Song KY, Wang HJ, Hua RH, Yu YX: A single nucleotide mutation in NS2A of Japanese encephalitis-live vaccine virus (SA14-14-2) ablates NS1’ formation and contributes to attenuation. J Gen Virol 2012, 93: 1959-1964. 10.1099/vir.0.043844-0PubMedCrossRef
35.
Huang Y, Liu S, Yang P, Wang C, Du Y, Yu W, Sun Z: Replicon-based Japanese encephalitis virus vaccines elicit immune response in mice. J Virol Methods 2012, 179: 217-225. 10.1016/j.jviromet.2011.11.002PubMedCrossRef
36.
Huang Y, Liu S, Yang P, Wang C, Du Y, Sun Z, Yu W: Influence of Japanese enciphalitis virus capsid protein on the self-replicate ability of JEV replicon vectors. Sheng Wu Gong Cheng Xue Bao 2010, 26: 1088-1094.PubMed
37.
Hennessy S, Liu Z, Tsai TF, Strom BL, Wan CM, Liu HL, Wu TX, Yu HJ, Liu QM, Karabatsos N: Effectiveness of live-attenuated Japanese encephalitis vaccine (SA14-14-2): a case–control study. Lancet 1996, 347: 1583-1586. 10.1016/S0140-6736(96)91075-2PubMedCrossRef
38.
Tandan JB, Ohrr H, Sohn YM, Yoksan S, Ji M, Nam CM, Halstead SB: Single dose of SA 14-14-2 vaccine provides long-term protection against Japanese encephalitis: a case–control study in Nepalese children 5 years after immunization. Vaccine 2007, 25: 5041-5045. 10.1016/j.vaccine.2007.04.052PubMedCrossRef
39.
Ohrr H, Tandan JB, Sohn YM, Shin SH, Pradhan DP, Halstead SB: Effect of single dose of SA 14-14-2 vaccine 1 year after immunisation in Nepalese children with Japanese encephalitis: a case–control study. Lancet 2005, 366: 1375-1378. 10.1016/S0140-6736(05)67567-8PubMedCrossRef
40.
Franco D, Li W, Qing F, Stoyanov CT, Moran T, Rice CM, Ho DD: Evaluation of yellow fever virus 17D strain as a new vector for HIV-1 vaccine development. Vaccine 2010, 28: 5676-5685. 10.1016/j.vaccine.2010.06.052PubMedCrossRef
41.
McGee CE, Lewis MG, Claire MS, Wagner W, Lang J, Guy B, Tsetsarkin K, Higgs S, Decelle T: Recombinant chimeric virus with wild-type dengue 4 virus premembrane and envelope and virulent yellow fever virus Asibi backbone sequences is dramatically attenuated in nonhuman primates. J Infect Dis 2008, 197: 693-697. 10.1086/527329PubMedCrossRef
42.
Chambers TJ, Liang Y, Droll DA, Schlesinger JJ, Davidson AD, Wright PJ, Jiang X: Yellow fever virus/dengue-2 virus and yellow fever virus/dengue-4 virus chimeras: biological characterization, immunogenicity, and protection against dengue encephalitis in the mouse model. J Virol 2003, 77: 3655-3668. 10.1128/JVI.77.6.3655-3668.2003PubMedPubMedCentralCrossRef
43.
van Der Most RG, Murali-Krishna K, Ahmed R, Strauss JH: Chimeric yellow fever/dengue virus as a candidate dengue vaccine: quantitation of the dengue virus-specific CD8 T-cell response. J Virol 2000, 74: 8094-8101. 10.1128/JVI.74.17.8094-8101.2000PubMedPubMedCentralCrossRef
44.
Guirakhoo F, Weltzin R, Chambers TJ, Zhang ZX, Soike K, Ratterree M, Arroyo J, Georgakopoulos K, Catalan J, Monath TP: Recombinant chimeric yellow fever-dengue type 2 virus is immunogenic and protective in nonhuman primates. J Virol 2000, 74: 5477-5485. 10.1128/JVI.74.12.5477-5485.2000PubMedPubMedCentralCrossRef
45.
Guirakhoo F, Zhang ZX, Chambers TJ, Delagrave S, Arroyo J, Barrett AD, Monath TP: Immunogenicity, genetic stability, and protective efficacy of a recombinant, chimeric yellow fever-Japanese encephalitis virus (ChimeriVax-JE) as a live, attenuated vaccine candidate against Japanese encephalitis. Virology 1999, 257: 363-372. 10.1006/viro.1999.9695PubMedCrossRef
46.
Chambers TJ, Droll DA, Jiang X, Wold WS, Nickells JA: JE Nakayama/JE SA14-14-2 virus structural region intertypic viruses: biological properties in the mouse model of neuroinvasive disease. Virology 2007, 366: 51-61. 10.1016/j.virol.2007.04.016PubMedPubMedCentralCrossRef
47.
Yun SI, Song BH, Koo Y, Jeon I, Byun SJ, Park JH, Joo YS, Kim SY, Lee YM: Japanese encephalitis virus-based replicon RNAs/particles as an expression system for HIV-1 Pr55 Gag that is capable of producing virus-like particles. Virus Res 2009, 144: 298-305. 10.1016/j.virusres.2009.04.014PubMedCrossRef
Metadata
Title
Development and characterization of the replicon system of Japanese encephalitis live vaccine virus SA14-14-2
Authors
Shi-Hua Li
Xiao-Feng Li
Hui Zhao
Yong-Qiang Deng
Xue-Dong Yu
Shun-Ya Zhu
Tao Jiang
Qing Ye
E-De Qin
Cheng-Feng Qin
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2013
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-10-64

Other articles of this Issue 1/2013

Virology Journal 1/2013 Go to the issue

Research

A seroepidemiological survey of Crimean Congo hemorrhagic fever among Cattle in North Kordufan State, Sudan

Research

Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in cervical cancer

Methodology

High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes

Research

Quantitative real-time PCR detection of Zika virus and evaluation with field-caught Mosquitoes

Research

Neutralization sensitivity of HIV-1 subtype B’ clinical isolates from former plasma donors in China

Research

Quantitative detection of relative expression levels of the whole genome of Southern rice black-streaked dwarf virus and its replication in different hosts
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits