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BMC Gastroenterology
Published in: BMC Gastroenterology 1/2012

Open Access 01-12-2012 | Research article

hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions

Authors: Tanielly Cristina Raiol Silva, Mariana Ferreira Leal, Danielle Queiroz Calcagno, Carolina Rosal Teixeira de Souza, André Salim Khayat, Ney Pereira Carneiro dos Santos, Raquel Carvalho Montenegro, Silvia Helena Barem Rabenhorst, Mayara Quaresma Nascimento, Paulo Pimentel Assumpção, Marília de Arruda Cardoso Smith, Rommel Rodríguez Burbano

Published in: BMC Gastroenterology | Issue 1/2012

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Abstract

Background

Gastric cancer is a serious public health problem in Northern Brazil and in the world due to its high incidence and mortality. Despite the severity of the disease, more research is needed to better understand the molecular events involved in this intestinal-type gastric carcinogenesis process. Since precancerous lesions precede intestinal-type gastric cancer, here, we evaluated the hTERT, MYC, and TP53 mRNA and protein expression, as well as TP33 copy number, in gastric preneoplastic lesions.

Methods

We evaluated 19 superficial gastritis, 18 atrophic gastritis, and 18 intestinal metaplasia from cancer-free individuals of Northern Brazil. Quantitative reverse transcription PCR was used to analyze the mRNA expression and immunohistochemical methods were used to assess protein immunoreactivity in tissue samples. The number of TP53 gene copies was investigated in gastric diseases by quantitative PCR.

Results

We observed hTERT, MYC, and p53 immunoreactivity only in intestinal metaplasia samples. The immunoreactivity of these proteins was strongly associated with each other. A significantly higher MYC mRNA expression was observed in intestinal metaplasia compared to gastritis samples. Loss of TP53 was also only detected in intestinal metaplasia specimens.

Conclusions

We demonstrated that hTERT, MYC, and TP53 are deregulated in intestinal metaplasia of individuals from Northern Brazil and these alterations may facilitate tumor initiation.
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Metadata
Title
hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions
Authors
Tanielly Cristina Raiol Silva
Mariana Ferreira Leal
Danielle Queiroz Calcagno
Carolina Rosal Teixeira de Souza
André Salim Khayat
Ney Pereira Carneiro dos Santos
Raquel Carvalho Montenegro
Silvia Helena Barem Rabenhorst
Mayara Quaresma Nascimento
Paulo Pimentel Assumpção
Marília de Arruda Cardoso Smith
Rommel Rodríguez Burbano
Publication date
01-12-2012
Publisher
BioMed Central
Published in
BMC Gastroenterology / Issue 1/2012
Electronic ISSN: 1471-230X
DOI
https://doi.org/10.1186/1471-230X-12-85

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