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Archives of Virology
Published in: Archives of Virology 1/2009

01-01-2009 | Original Article

HPV16 E6 oncoprotein increases cell adhesion in human keratinocytes

Authors: Alexander Epshtein, A. Jackman, P. Gonen, L. Sherman

Published in: Archives of Virology | Issue 1/2009

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Abstract

Expression of the E6 oncoprotein of human papillomavirus (HPV) 16 in primary human keratinocytes (PHKs) was previously shown to significantly reduce apoptosis. This could be due to increased cell adhesion. Adhesion ability was tested by seeding cells on tissue culture dishes coated with different concentrations of poly(HEME) and determination of the proportion of attached cells. Assays were carried out with PHKs, immortalized human keratinocytes (HaCaT) and human 293T cells. The E6 gene was transduced via retroviral infection or DNA transfection. Results of these assays showed that expression of E6 increased the proportion of cells that attached to poly(HEME). Several HPV16 E6 mutants were also tested in the above assay in 293T cells. These assays showed that the p53 targeting region of E6 is dispensable for this activity. Assays of inhibition of tyrosine kinases by bombesin showed that E6 probably utilizes other pathways to increase cell adhesion.
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Metadata
Title
HPV16 E6 oncoprotein increases cell adhesion in human keratinocytes
Authors
Alexander Epshtein
A. Jackman
P. Gonen
L. Sherman
Publication date
01-01-2009
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 1/2009
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-008-0273-9

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