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01-04-2019 | Symposium-in-Writing Paper

How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions

Authors: Annika M. Bruger, Anca Dorhoi, Gunes Esendagli, Katarzyna Barczyk-Kahlert, Pierre van der Bruggen, Marie Lipoldova, Tomas Perecko, Juan Santibanez, Margarida Saraiva, Jo A. Van Ginderachter, Sven Brandau

Published in: Cancer Immunology, Immunotherapy | Issue 4/2019

Abstract

Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of mononuclear and polymorphonuclear myeloid cells, which are present at very low numbers in healthy subjects, but can expand substantially under disease conditions. Depending on disease type and stage, MDSC comprise varying amounts of immature and mature differentiation stages of myeloid cells. Validated unique phenotypic markers for MDSC are still lacking. Therefore, the functional analysis of these cells is of central importance for their identification and characterization. Various disease-promoting and immunosuppressive functions of MDSC are reported in the literature. Among those, the capacity to modulate the activity of T cells is by far the most often used and best-established read-out system. In this review, we critically evaluate the assays available for the functional analysis of human and murine MDSC under in vitro and in vivo conditions. We also discuss critical issues and controls associated with those assays. We aim at providing suggestions and recommendations useful for the contemporary biological characterization of MDSC.

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Title: How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions
Authors: Annika M. Bruger
Anca Dorhoi
Gunes Esendagli
Katarzyna Barczyk-Kahlert
Pierre van der Bruggen
Marie Lipoldova
Tomas Perecko
Juan Santibanez
Margarida Saraiva
Jo A. Van Ginderachter
Sven Brandau
Publication date: 01-04-2019
Publisher: Springer Berlin Heidelberg
Published in: Cancer Immunology, Immunotherapy / Issue 4/2019
Print ISSN: 0340-7004
Electronic ISSN: 1432-0851
DOI: https://doi.org/10.1007/s00262-018-2170-8

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