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Cancer Immunology, Immunotherapy
Published in: Cancer Immunology, Immunotherapy 4/2019

Open Access 01-04-2019 | Symposium-in-Writing Paper

Deciphering myeloid-derived suppressor cells: isolation and markers in humans, mice and non-human primates

Authors: Luca Cassetta, Espen S. Baekkevold, Sven Brandau, Anna Bujko, Marco A. Cassatella, Anca Dorhoi, Carsten Krieg, Ang Lin, Karin Loré, Olivia Marini, Jeffrey W. Pollard, Mikael Roussel, Patrizia Scapini, Viktor Umansky, Gosse J. Adema

Published in: Cancer Immunology, Immunotherapy | Issue 4/2019

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Abstract

In cancer, infection and inflammation, the immune system’s function can be dysregulated. Instead of fighting disease, immune cells may increase pathology and suppress host-protective immune responses. Myeloid cells show high plasticity and adapt to changing conditions and pathological challenges. Despite their relevance in disease pathophysiology, the identity, heterogeneity and biology of myeloid cells is still poorly understood. We will focus on phenotypical and functional markers of one of the key myeloid regulatory subtypes, the myeloid derived suppressor cells (MDSC), in humans, mice and non-human primates. Technical issues regarding the isolation of the cells from tissues and blood, timing and sample handling of MDSC will be detailed. Localization of MDSC in a tissue context is of crucial importance and immunohistochemistry approaches for this purpose are discussed. A minimal antibody panel for MDSC research is provided as part of the Mye-EUNITER COST action. Strategies for the identification of additional markers applying state of the art technologies such as mass cytometry will be highlighted. Such marker sets can be used to study MDSC phenotypes across tissues, diseases as well as species and will be crucial to accelerate MDSC research in health and disease.
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Metadata
Title
Deciphering myeloid-derived suppressor cells: isolation and markers in humans, mice and non-human primates
Authors
Luca Cassetta
Espen S. Baekkevold
Sven Brandau
Anna Bujko
Marco A. Cassatella
Anca Dorhoi
Carsten Krieg
Ang Lin
Karin Loré
Olivia Marini
Jeffrey W. Pollard
Mikael Roussel
Patrizia Scapini
Viktor Umansky
Gosse J. Adema
Publication date
01-04-2019
Publisher
Springer Berlin Heidelberg
Published in
Cancer Immunology, Immunotherapy / Issue 4/2019
Print ISSN: 0340-7004
Electronic ISSN: 1432-0851
DOI
https://doi.org/10.1007/s00262-019-02302-2

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Keynote webinar | Spotlight on antibody–drug conjugates in cancer

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Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras
Prof. Fabrice Barlesi
Developed by: Springer Medicine
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