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01-08-2003 | Original Article

Histamine-degrading enzymes as cellular markers of acute small bowel allograft rejection

Authors: Alexander Klaus, Helmut Weiss, Justin H. Nguyen, Raimund Margreiter, Peter Obrist, Hubert G. Schwelberger

Published in: Transplant International | Issue 8/2003

Abstract

Intestinal histamine-degrading enzymes diamine oxidase (DAO) and histamine N-methyltransferase (HNMT) activities are relatively constant per individual and bowel segment, and they reflect the functional integrity of the intestinal mucosa. It was, therefore, hypothesised that a decrease in these enzymes could be indicative of acute rejection of an intestinal allograft. Enzymatic activities of DAO and HNMT were determined in mucosal biopsies of isogeneic (Lewis-to-Lewis, n=48) and allogeneic (Brown Norway-to-Lewis, n=48) heterotopic small bowel transplants in a rat model at various time periods. Allograft recipients were not given any immunosuppression. While no changes in enzyme activities were observed in isografts up to day 8 following transplantation, significantly reduced activities of both enzymes were found in all allografts 6–8 days after transplantation. Activities of both DAO and HNMT exhibited a strong negative correlation with the histological rejection score (P<0.01). We can conclude that DAO and HNMT activities in gut mucosa are reliable quantitative markers of acute intestinal allograft rejection in the rat that support histopathological analysis.

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Title: Histamine-degrading enzymes as cellular markers of acute small bowel allograft rejection
Authors: Alexander Klaus
Helmut Weiss
Justin H. Nguyen
Raimund Margreiter
Peter Obrist
Hubert G. Schwelberger
Publication date: 01-08-2003
Publisher: Springer-Verlag
Published in: Transplant International / Issue 8/2003
Print ISSN: 0934-0874
Electronic ISSN: 1432-2277
DOI: https://doi.org/10.1007/s00147-003-0589-z

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Histamine-degrading enzymes as cellular markers of acute small bowel allograft rejection

Abstract

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Abstract

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