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01-08-2003 | Original Article

Induction of indirect donor-specific hyporesponsiveness by transportal RT1-peptide pulse in rat skin transplantation

Authors: Masao Yamamura, Takahito Yagi, Hiromi Iwagaki, Naoshi Mitsuoka, Liu Jie, Sun Dong Sheng, Hiroaki Matsuda, Hiroshi Sadamori, Masaru Inagaki, Noriaki Tanaka

Published in: Transplant International | Issue 8/2003

Abstract

In the present study, we examined whether transportal pulse of class I major histocompatibility complex (MHC) allopeptides can induce indirect (non-chimeric) donor-specific hyporesponsiveness, using a high-responder rat skin transplantation model. Two donor-specific 8-amino acid peptides corresponding to residues 58–65 and 70–77 in the α₁ helical region of RT1.A^a were synthesized. In order to test immunogenicity of these peptides, mixed lymphocyte reaction (MLR) was performed. Then, 100-μg portions of peptides were injected into recipient Lewis (LEW, RT1.A^l) rats via the portal vein 14 days before skin transplantation. Skin allografts from August Copenhagen Irish (ACI, RT1^a) or Wistar King A (WKA, RT1^k, third-party) donors were transplanted to LEW (RT1^l) recipients. Transportal pulse of residues 58–65 and 70–77 prolonged graft survival significantly in ACI-to-LEW skin transplantation (17.6±0.40 and 18.0±0.45 days) compared with control (14.2±0.37 days). However, pulse of residues 106–113, a non-donor-specific control, did not prolong graft survival time (14.6±0.40 days) in the same combination. Regarding the third-party donor, residues 58–65 injected into LEW recipients had no effect on survival time of skin grafts (19.0±0.84 days) derived from WKA donors compared with the untreated WKA-to-LEW control (19.4±0.93 days). Transportal pulse of RT1.A^a peptides induced donor-specific hyporesponsiveness even in a high-responder rat skin transplantation model. Our results suggest that graft enhancement by transportal exposure to donor cells may not be induced by a chimeric process but, instead, by an indirect mechanism not involving intervention of viable donor cells.

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Title: Induction of indirect donor-specific hyporesponsiveness by transportal RT1-peptide pulse in rat skin transplantation
Authors: Masao Yamamura
Takahito Yagi
Hiromi Iwagaki
Naoshi Mitsuoka
Liu Jie
Sun Dong Sheng
Hiroaki Matsuda
Hiroshi Sadamori
Masaru Inagaki
Noriaki Tanaka
Publication date: 01-08-2003
Publisher: Springer-Verlag
Published in: Transplant International / Issue 8/2003
Print ISSN: 0934-0874
Electronic ISSN: 1432-2277
DOI: https://doi.org/10.1007/s00147-002-0496-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Induction of indirect donor-specific hyporesponsiveness by transportal RT1-peptide pulse in rat skin transplantation

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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