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Archives of Virology
Published in: Archives of Virology 7/2017

01-07-2017 | Original Article

High-throughput sequencing identifies HIV-1-replication- and latency-related miRNAs in CD4+ T cell lines

Authors: Xiangyun Lu, Jin Yang, Haibo Wu, Zongxing Yang, Changzhong Jin, Juan Wang, Linfang Cheng, Xiaorong Peng, Fumin Liu, Xiuming Peng, Sujing Ji, Huilin Ou, Tiansheng Xie, Hangping Yao, Nanping Wu

Published in: Archives of Virology | Issue 7/2017

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Abstract

MicroRNAs are potent gene expression regulators involved in regulating various biological processes, including host-pathogen interactions. In this study, we used high-throughput sequencing to investigate cellular miRNA signatures related to HIV-1 replication and latent infection in CD4+ T cell lines, which included HIV-1-replicating H9/HTLV-IIIB, HIV-1-latently-infected CEM-Bru cells, and their parental uninfected H9 and CEM-SS cells. Relatively few miRNAs were found to be modulated by HIV-1 replication or latent infection, while the cell-lineage-specific miRNA difference was more pronounced, irrespective of HIV-1 infection. In silico analysis showed that some of our HIV-1 infection-regulated miRNA profiles echoed previous studies, while others were novel. In addition, some of the miRNAs that were differentially expressed between the productively and latently infected cells seemed to participate in shaping the differential infection state. Thus, the newly identified miRNA profiles related to HIV-1 replication and latency provide information about the interplay between HIV-1 and its host.
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Metadata
Title
High-throughput sequencing identifies HIV-1-replication- and latency-related miRNAs in CD4+ T cell lines
Authors
Xiangyun Lu
Jin Yang
Haibo Wu
Zongxing Yang
Changzhong Jin
Juan Wang
Linfang Cheng
Xiaorong Peng
Fumin Liu
Xiuming Peng
Sujing Ji
Huilin Ou
Tiansheng Xie
Hangping Yao
Nanping Wu
Publication date
01-07-2017
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 7/2017
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-017-3305-5

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