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Tumor Biology
Published in: Tumor Biology 10/2016

01-10-2016 | Original Article

High expression of apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) in human cholangiocarcinoma

Authors: Daraporn Chua-on, Tanakorn Proungvitaya, Anchalee Techasen, Temduang Limpaiboon, Sittiruk Roytrakul, Sopit Wongkham, Chaisiri Wongkham, Ongart Somintara, Sakkarn Sungkhamanon, Siriporn Proungvitaya

Published in: Tumor Biology | Issue 10/2016

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Abstract

Prognosis of cholangiocarcinoma (CCA) patients is absolutely poor in spite of extensive efforts for the development of chemotherapy. Mitochondrial proteins play key roles in carcinogenesis of various cancers. Therefore, mitochondria are considered as the target organelles for chemotherapy of several cancers including CCA. The purpose of this study is to identify potential candidate proteins for chemotherapy using mitochondrial proteome analysis for CCA tissues. A shotgun proteomic approach using SDS-PAGE coupled with LC-MS/MS was applied to compare the expression of mitochondrial proteins in CCA and the adjacent non-cancerous tissues. Using the proteomic analysis for the pooled mitochondrial proteins purified from three each of papillary and non-papillary types of CCA and their adjacent tissues, 281 proteins were identified as mitochondrial proteins, and 105 of them have significantly different expression levels compared with the corresponding counterparts. Among the 105 proteins, apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) was a unique protein commonly over-expressed in both papillary and non-papillary types of CCA tissues but not in the adjacent non-cancerous tissues. In conclusion, AIFM3 was aberrantly expressed only in the mitochondria of CCA tissues. This finding suggests that AIFM3 could be a potential target molecule for CCA chemotherapy.
Literature
1.
2.
Sripa B, Tangkawattana S, Laha T, Kaewkes S, Mallory FF, Smith JF, et al. Toward integrated opisthorchiasis control in Northeast Thailand: the Lawa project. Acta Trop. 2015;141(Pt B):361–7.CrossRefPubMed
3.
Woradet S, Promthet S, Songserm N, Parkin DM. Factors affecting survival time of cholangiocarcinoma patients: a prospective study in Northeast Thailand. Asian Pac J Cancer Prev. 2013;14(3):1623–7.CrossRefPubMed
4.
5.
Yusenko MV, Ruppert T, Kovacs G. Analysis of differentially expressed mitochondrial proteins in chromophobe renal cell carcinomas and renal oncocytomas by 2-D gel electrophoresis. Int J Biol Sci. 2010;6(3):213–24.CrossRefPubMedPubMedCentral
6.
Kim HK, Park WS, Kang SH, Warda M, Kim N, Ko JH, et al. Mitochondrial alterations in human gastric carcinoma cell line. Am J Phys Cell Physiol. 2007;293(2):C761–71.CrossRef
7.
McGee AM, Douglas DL, Liang Y, Hyder SM, Baines CP. The mitochondrial protein C1qbp promotes cell proliferation, migration and resistance to cell death. Cell Cycle. 2011;10(23):4119–27.CrossRefPubMedPubMedCentral
8.
Miyasaka Y, Enomoto N, Nagayama K, Izumi N, Marumo F, Watanabe M, et al. Analysis of differentially expressed genes in human hepatocellular carcinoma using suppression subtractive hybridization. Br J Cancer. 2001;85(2):228–34.CrossRefPubMedPubMedCentral
9.
Kuai XY, Ji ZY, Zhang HJ. Mitochondrial uncoupling protein 2 expression in colon cancer and its clinical significance. World J Gastroenterol. 2010;16(45):5773–8.CrossRefPubMedPubMedCentral
10.
Jain M, Nilsson R, Sharma S, Madhusudhan N, Kitami T, Souza AL, et al. Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science. 2012;336(6084):1040–4.CrossRefPubMedPubMedCentral
11.
Wadhwa R, Yaguchi T, Hasan MK, Taira K, Kaul SC. Mortalin-MPD (mevalonate pyrophosphate decarboxylase) interactions and their role in control of cellular proliferation. Biochem Biophys Res Commun. 2003;302(4):735–42.CrossRefPubMed
12.
Sawai M, Yashiro M, Nishiguchi Y, Ohira M, Hirakawa K. Growth-inhibitory effects of the ketone body, monoacetoacetin, on human gastric cancer cells with succinyl-CoA: 3-oxoacid CoA-transferase (SCOT) deficiency. Anticancer Res. 2004;24(4):2213–7.PubMed
13.
Kongpetch S, Kukongviriyapan V, Prawan A, Senggunprai L, Kukongviriyapan U, Buranrat B. Crucial role of heme oxygenase-1 on the sensitivity of cholangiocarcinoma cells to chemotherapeutic agents. PLoS One. 2012;7(4):e34994.CrossRefPubMedPubMedCentral
14.
Okaro A, Fennell D, Corbo M, Davidson B, Cotter F. Pk11195, a mitochondrial benzodiazepine receptor antagonist, reduces apoptosis threshold in Bcl-XL and mcl-1 expressing human cholangiocarcinoma cells. Gut. 2002;51:556–61.CrossRefPubMedPubMedCentral
15.
Giris M, Erbil Y, Depboylu B, Mete O, Turkoglu U, Abbasoglu SD, et al. Heme oxygenase-1 prevents hyperthyroidism induced hepatic damage via an antioxidant and antiapoptotic pathway. J Surg Res. 2010;164(2):266–75.CrossRefPubMed
16.
Loilome W, Kadsanit S, Namwat N, Techasen A, Puapairoj A, Dechakhamphu A, et al. Impaired antioxidant enzyme activity and increased DNA repair enzyme expression in hamster liver tissues related to cholangiocarcinoma development. Asian Pac J Cancer Prev. 2012;13(Suppl):59–64.PubMed
17.
Muisuk K, Silsirivanit A, Imtawil K, Bunthot S, Pukhem A, Pairojkul C, et al. Novel mutations in cholangiocarcinoma with low frequencies revealed by whole mitochondrial genome sequencing. Asian Pac J Cancer Prev. 2015;16(5):1737–42.CrossRefPubMed
18.
19.
Lee JH, Hong CS, Lee S, Yang JE, Park YI, Lee D, et al. Radiating amyloid fibril formation on the surface of lipid membranes through unit-assembly of oligomeric species of a-synuclein. PLoS One. 2012;7:e47580.CrossRefPubMedPubMedCentral
20.
Fernandez-Vizarra E, Lopez-Perez MJ, Enriquez JA. Isolation of biogenetically competent mitochondria from mammalian tissues and cultured cells. Methods. 2002;26(4):292–7.CrossRefPubMed
21.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265–75.PubMed
22.
Kuhn M, Szklarczyk D, Pletscher-Frankild S, Blicher TH, von Mering C, Jensen LJ, et al. STITCH 4: integration of protein-chemical interactions with user data. Nucleic Acids Res. 2014;42(Database issue):D401–7.CrossRefPubMed
23.
Ma HY, YN L, Marchbanks PA, Folger SG, Strom BL, McDonald JA, et al. Quantitative measures of estrogen receptor expression in relation to breast cancer-specific mortality risk among white women and black women. Breast Cancer Res. 2013;15(5):R90.CrossRefPubMedPubMedCentral
24.
Singh R, Avliyakulov NK, Braga M, Haykinson MJ, Martinez L, Singh V, et al. Proteomic identification of mitochondrial targets of arginase in human breast cancer. PLoS One. 2013;8(11):e79242.CrossRefPubMedPubMedCentral
25.
Xie Q, Lin T, Zhang Y, Zheng J, Bonanno JA. Molecular cloning and characterization of a human AIF-like gene with ability to induce apoptosis. J Biol Chem. 2005;280(20):19673–81.CrossRefPubMed
26.
Urbano A, Lakshmanan U, Choo PH, Kwan JC, Ng PY, Guo K, et al. AIF suppresses chemical stress-induced apoptosis and maintains the transformed state of tumor cells. EMBO J. 2005;24(15):2815–26.CrossRefPubMedPubMedCentral
27.
Klein JA, Longo-Guess CM, Rossmann MP, Seburn KL, Hurd RE, Frankel WN, et al. The harlequin mouse mutation downregulates apoptosis-inducing factor. Nature. 2002;419(6905):367–74.CrossRefPubMed
28.
Yang W, Sun T, Cao J, Liu F, Tian Y, Zhu W. Downregulation of miR-210 expression inhibits proliferation, induces apoptosis and enhances radiosensitivity in hypoxic human hepatoma cells in vitro. Exp Cell Res. 2012;318(8):944–54.CrossRefPubMed
29.
Yang W, Wei J, Sun T, Liu F. Effects of knockdown of miR-210 in combination with ionizing radiation on human hepatoma xenograft in nude mice. Radiat Oncol. 2013;8:102.CrossRefPubMedPubMedCentral
30.
Delettre C, Yuste VJ, Moubarak RS, Bras M, Lesbordes-Brion JC, Petres S, et al. AIFsh, a novel apoptosis-inducing factor (AIF) pro-apoptotic isoform with potential pathological relevance in human cancer. J Biol Chem. 2006;281(10):6413–27.CrossRefPubMed
31.
Wang R, Dashwood WM, Nian H, Lohr CV, Fischer KA, Tsuchiya N, et al. NADPH oxidase overexpression in human colon cancers and rat colon tumors induced by 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP). Int J Cancer. 2011;128(11):2581–90.CrossRefPubMed
32.
Li X, Pan Y, Fan R, Jin H, Han S, Liu J, et al. Adenovirus-delivered CIAPIN1 small interfering RNA inhibits HCC growth in vitro and in vivo. Carcinogenesis. 2008;29(8):1587–93.CrossRefPubMedPubMedCentral
33.
Magnifico A, Albano L, Campaner S, Campiglio M, Pilotti S, Menard S, et al. Protein kinase c alpha determines HER2 fate in breast carcinoma cells with HER2 protein overexpression without gene amplification. Cancer Res. 2007;67(11):5308–17.CrossRefPubMed
34.
Sun XM, MacFarlane M, Zhuang J, Wolf BB, Green DR, Cohen GM. Distinct caspase cascades are initiated in receptor-mediated and chemical-induced apoptosis. J Biol Chem. 1999;274(8):5053–60.CrossRefPubMed
35.
Wolf BB, Goldstein JC, Stennicke HR, Beere H, Amarante-Mendes GP, Salvesen GS, et al. Calpain functions in a caspase-independent manner to promote apoptosis-like events during platelet activation. Blood. 1999;94(5):1683–92.PubMed
36.
Zhang C, Feng YS, SL Q, Wei X, Zhu HL, Luo Q, et al. Resveratrol attenuates doxorubicin-induced cardiomyocyte apoptosis in mice through SIRT1-mediated deacetylation of p53. Cardiovasc Res. 2011;90(3):538–45.CrossRefPubMed
37.
YR F, Yi ZJ, Yan YR, Qiu ZY. Proteomic analysis of mitochondrial proteins in hydroxycamptothecin-treated SMMC-7721 cells. Zhonghua Gan Zang Bing Za Zhi. 2007;15(8):572–6.
38.
Diz AP, Truebano M, Skibinski DO. The consequences of sample pooling in proteomics: an empirical study. Electrophoresis. 2009;30(17):2967–75.CrossRefPubMed
Metadata
Title
High expression of apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) in human cholangiocarcinoma
Authors
Daraporn Chua-on
Tanakorn Proungvitaya
Anchalee Techasen
Temduang Limpaiboon
Sittiruk Roytrakul
Sopit Wongkham
Chaisiri Wongkham
Ongart Somintara
Sakkarn Sungkhamanon
Siriporn Proungvitaya
Publication date
01-10-2016
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 10/2016
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-016-5204-x

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