Top

Published in:

01-06-2020 | Herpes Virus | Short Communication

Genetic Analysis of Chelonid Herpesvirus 5 in Marine Turtles from Baja California Peninsula

Authors: Joelly Espinoza, Elsa Hernández, María Mónica Lara-Uc, Eduardo Reséndiz, Alonzo Alfaro-Núñez, Sawako Hori-Oshima, Gerardo Medina-Basulto

Published in: EcoHealth | Issue 2/2020

Abstract

The Chelonid herpesvirus 5 (ChHV5) is the primary etiological agent associated with fibropapillomatosis (FP), a neoplastic disease in marine turtles. In this study, we report for the first time ChHV5 in marine turtles and a leech from Baja California Peninsula. Eighty-seven black, olive or loggerhead turtle species, one FP tumor and five leeches were analyzed. The tumor sample from an olive, a skin sample from a black and a leech resulted positive of ChHV5 for conventional PCR. Two viral variants were identified and grouped within the Eastern Pacific phylogenetic group, suggesting a possible flow of the virus in this region.

Available only for authorised users

Aguirre AA, Lutz P (2004) Marine turtles as sentinels of ecosystem health: is fibropapillomatosis an indicator? EcoHealth 1:275–283. https://doi.org/10.1007/s10393-004-0097-3CrossRef

Alfaro-Núñez A, Gilbert MTP (2014) Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections. Journal of Virological Methods 206C:38–41. https://doi.org/10.1016/j.jviromet.2014.05.019CrossRef

Alfaro-Núñez A, Frost Bertelsen M, Bojesen A, Rasmussen I, Zepeda-Mendoza L, Tange Olsen M, Gilbert M (2014) Global distribution of Chelonid fibropapilloma-associated herpesvirus among clinically healthy sea turtles. BMC Evolutionary Biology 14:206. https://doi.org/10.1186/s12862-014-0206-zCrossRefPubMedPubMedCentral

Alfaro-Núñez A, Bojesen AM, Bertelsen MF, Wales N, Balazs GH, Gilbert MTP (2016) Further evidence of Chelonid herpesvirus 5 (ChHV5) latency: high levels of ChHV5 DNA detected in clinically healthy marine turtles. PeerJ 4:e2274. https://doi.org/10.7717/peerj.2274CrossRefPubMedPubMedCentral

Ariel E, Nainu F, Jones K, Juntunen K, Bell I, Gaston J, Scott J, Trocini S, Burgess GW (2017) Phylogenetic Variation of Chelonid Alphaherpesvirus 5 (ChHV5) in Populations of Green Turtles Chelonia mydas along the Queensland Coast, Australia. Journal of Aquatic Animal Health 29:150–157. https://doi.org/10.1177/0300985814560236CrossRefPubMed

Barragan A, Sarti LM (1994) A Possible Case of Fibropapilloma in Kemp’s Ridley Turtle (Lepidochelys kempii). Marine Turtle Newsletter 67:1

Brenes Chaves L, Berrocal A, Meneses AI, Jiménez Sánchez C, Orrego Vásquez CM (2013) Study on the etiology of fibropapillomatosis of olive ridley sea turtles (Lepidochelys olivacea) nesting in the National Wildlife Refuge at Ostional, Guanacaste, Costa Rica. Revista Ciencias Marinas y Costeras 5:119. https://doi.org/10.15359/revmar.10-5.8CrossRef

Cárdenas DM, Cucalón RV, Medina-Magües LG, Jones K, Alemán RA, Alfaro-Núñez A, Cárdenas WB (2019) Fibropapillomatosis in a Green Sea Turtle (Chelonia mydas) from the Southeastern Pacific. Journal of Wildlife Diseases 55:169–173. https://doi.org/10.7589/2017-12-295CrossRefPubMed

Chaves A, Aguirre AA, Blanco-Peña K, Moreira-Soto A, Monge O, Torres AM, Soto-Rivas JL, Lu Y, Chacón D, Fonseca L, Jiménez M, Gutiérrez-Espeleta G, Lierz M (2017) Examining the role of transmission of chelonid alphaherpesvirus 5. EcoHealth 14:530–541. https://doi.org/10.1177/0300985814560236CrossRefPubMed

Santos dos RG, Martins AS, Torezani E, Baptistotte C, Farias J, Horta PA, Work TM, Balazs GH (2010) Relationship between fibropapillomatosis and environmental quality: a case study with Chelonia mydas off Brazil. Diseases of Aquatic Organisms 89:87–95. https://doi.org/10.3354/dao02178CrossRefPubMed

Drummond AJ, Rambaut A (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology 7:214. https://doi.org/10.1186/1471-2148-7-214CrossRefPubMedPubMedCentral

Ene A, Su M, Lemaire S, Rose C, Schaff S, Moretti R, Lenz J, Herbst LH (2005) Distribution of chelonid fibropapillomatosis-associated herpesvirus variants in Florida: molecular genetic evidence for infection of turtles following recruitment to neritic developmental habitats. Journal of Wildlife Diseases 41:489–497. https://doi.org/10.7589/0090-3558-41.3.489CrossRefPubMed

Greenblatt RJ, Work TM, Balazs GH, Sutton CA, Casey RN, Casey JW (2004) The Ozobranchus leech is a candidate mechanical vector for the fibropapilloma-associated turtle herpesvirus found latently infecting skin tumors on Hawaiian green turtles (Chelonia mydas). Virology 321:101–110. https://doi.org/10.1016/j.virol.2003.12.026CrossRefPubMed

Greenblatt RJ, Quackenbush SL, Casey RN, Rovnak J, Balazs GH, Work TM, Casey JW, Sutton CA (2005) Genomic variation of the fibropapilloma-associated marine turtle herpesvirus across seven geographic areas and three host species. Journal of Virology 79:1125–1132. https://doi.org/10.1128/jvi.79.2.1125-1132.2005CrossRefPubMedPubMedCentral

Herbst LH (1994) Fibropapillomatosis of marine turtles. Annual Review of Fish Diseases 4:389–425CrossRef

Herbst LH, Jacobson ER, Moretti RH, Brown T, Sundberg JP, Klein PA (1995) Experimental transmission of green turtle fibropapillomatosis using cell-free tumor extracts. Diseases of Aquatic Organisms 22:1–12CrossRef

Herbst LH, Ene A, Su M, DeSalle R, Lenz J (2004) Tumor outbreaks in marine turtles are not due to recent herpesvirus mutations. Current Biology 14:R697–R699. https://doi.org/10.1016/j.cub.2004.08.040CrossRefPubMed

Huerta P, Pineda H, Aguirre A, Spraker T, Sarti L, Barragán A (2002) First confirmed case of fibropapilloma in a leatherback turtle (Dermochelys coriacea). In: Mosier A, Foley A, Brost B (eds) Proceedings of the 20th annual symposium on sea turtle biology and conservation, p 193

Jacobson ER, Buergett C, Williams B, Harris RK (1991) Herpesvirus in cutaneous fibropapillomas of the green turtle Chelonia mydas. Diseases of Aquatic Organisms 12:1–6CrossRef

Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649. https://doi.org/10.1093/bioinformatics/bts199CrossRefPubMedPubMedCentral

Lackovich JK, Brown DR, Homer BL, Garber RL, Mader DR, Moretti RH, Patterson AD, Herbst LH, Oros J, Jacobson ER, Curry SS, Klein PA (1999) Association of herpesvirus with fibropapillomatosis of the green turtle Chelonia mydas and the loggerhead turtle Caretta caretta in Florida. Diseases of Aquatic Organisms 37:89–97. https://doi.org/10.3354/dao037089CrossRefPubMed

Li T-H, Hsu W-L, Lan Y-C, Balazs GH, Work TM, Tseng C-T, Chang C-C (2017) Identification of Chelonid herpesvirus 5 (ChHV5) in endangered green turtles (Chelonia mydas) with fibropapillomatosis in Asia. Bulletin of Marine Science 93:1011–1022. https://doi.org/10.5343/bms.2017.1018CrossRef

Limpus CJ, Walter DG (1980) The growth of immature green turtles (Chelonia mydas) under natural conditions. In: Herpetologica. https://www.jstor.org/stable/3891481?seq=1. Accessed 25 Jan 2020

A Maldonado-Gasca, Zapata-Rosales M (2007) Primeros registros de tortugas blancas Chelonia mydas con fibropapillomas en Yucatán, México. CICIMAR Oceánides 22:29–33

Monezi TA, Mehnert DU, de Moura EMM, Müller NMG, Garrafa P, Matushima ER, Werneck MR, Borella MI (2016) Chelonid herpesvirus 5 in secretions and tumor tissues from green turtles (Chelonia mydas) from Southeastern Brazil: A ten-year study. Veterinary Microbiology 186:150–156. https://doi.org/10.1016/j.vetmic.2016.02.020CrossRefPubMed

Page-Karjian A, Norton TM, Ritchie B, Brown C, Mancia C, Jackwood M, Gottdenker NL (2015) Quantifying chelonid herpesvirus 5 in symptomatic and asymptomatic rehabilitating green sea turtles. Endangered Species Research 28:135–146. https://doi.org/10.3354/esr00687CrossRef

Patricio AR, Herbst LH, Duarte A, Velez-Zuazo X, Santos Loureiro N, Pereira N, Tavares L, Toranzos GA (2012) Global phylogeography and evolution of chelonid fibropapilloma-associated herpesvirus. Journal of General Virology 93:1035–1045. https://doi.org/10.1099/vir.0.038950-0CrossRefPubMed

Quackenbush SL, Work TM, Balazs GH, Casey RN, Rovnak J, Chaves A, duToit L, Baines JD, Parrish CR, Bowser PR, Casey JW (1998) Three closely related herpesviruses are associated with fibropapillomatosis in marine turtles. Virology 246:392–399. https://doi.org/10.1006/viro.1998.9207CrossRefPubMed

Quackenbush SL, Casey RN, Murcek RJ, Paul TA, Work TM, Limpus CJ, Chaves A, duToit L, Perez JV, Aguirre AA, Spraker TR, Horrocks JA, Vermeer LA, Balazs GH, Casey JW (2001) Quantitative analysis of herpesvirus sequences from normal tissue and fibropapillomas of marine turtles with real-time PCR. Virology 287:105–111. https://doi.org/10.1006/viro.2001.1023CrossRefPubMed

Reséndiz E, Flores-Ramírez S, Koch V, Cordero-Tapia A (2016) First record of fibropapillomatosis in a green turtle Chelonia mydas from the Baja California Peninsula. Journal of Aquatic Animal Health 28:252–257. https://doi.org/10.1177/0300985814560236CrossRefPubMed

Rodenbusch CR, Baptistotte C, Werneck MR, Pires TT, Melo M, de Ataíde MW, Reis dos K, Testa P, Alieve MM, Canal CW (2014) Fibropapillomatosis in green turtles Chelonia mydas in Brazil: characteristics of tumors and virus. Diseases of Aquatic Organisms 111:207–217. https://doi.org/10.3354/dao02782CrossRefPubMed

Seminoff JA, Resendiz A, Nichols WJ (2002) Home range of green turtles Chelonia mydas at a coastal foraging area in the Gulf of California, Mexico. Marine Ecology Progress Series 242:253–265. https://doi.org/10.3354/meps242253CrossRef

Senko J, López-Castro MC, Koch V, Nichols WJ (2010) Immature east pacific green turtles (Chelonia mydas) use multiple foraging areas off the Pacific coast of Baja California Sur, Mexico: first evidence from mark-recapture data. BioOne 64:125–130. https://doi.org/10.2984/64.1.125CrossRef

Van Houtan KS, Smith CM, Dailer ML, Kawachi M (2014) Eutrophication and the dietary promotion of sea turtle tumors. PeerJ 2:e602. https://doi.org/10.7717/peerj.602CrossRefPubMedPubMedCentral

Vivaldo SG, Márquez L, Sarabia DO, García J, Casas FC (2009) Patología de las tortugas marinas (Lepidochelys olivacea) que arribaron a las playas de Cuyutlán, Colima, México. In: Veterinaria Mexico. http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922009000100007. Accessed 25 Jan 2009

Williams EH, Bunkley-Williams L, Casey JW (2006) Early fibropapillomas in Hawaii and occurrences in all sea turtle species: the panzootic, associated leeches wide-ranging on sea turtles, and species of study leeches should be identified. Journal of Virology 80:4643–4644. https://doi.org/10.1128/jvi.80.9.4643-4644.2006CrossRefPubMedPubMedCentral

Work TM, Dagenais J, Balazs GH, Schumacher J, Lewis TD, Leong J-AC, Casey RN, Casey JW (2009) In vitro biology of fibropapilloma-associated turtle herpesvirus and host cells in Hawaiian green turtles (Chelonia mydas). Journal of General Virology 90:1943–1950. https://doi.org/10.1099/vir.0.011650-0CrossRefPubMed

Title: Genetic Analysis of Chelonid Herpesvirus 5 in Marine Turtles from Baja California Peninsula
Authors: Joelly Espinoza
Elsa Hernández
María Mónica Lara-Uc
Eduardo Reséndiz
Alonzo Alfaro-Núñez
Sawako Hori-Oshima
Gerardo Medina-Basulto
Publication date: 01-06-2020
Publisher: Springer US
Keyword: Herpes Virus
Published in: EcoHealth / Issue 2/2020
Print ISSN: 1612-9202
Electronic ISSN: 1612-9210
DOI: https://doi.org/10.1007/s10393-020-01482-z

At a glance: The STEP trials

Springer Medicine

Genetic Analysis of Chelonid Herpesvirus 5 in Marine Turtles from Baja California Peninsula

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2020

Impact of Annual Bacterial Epizootics on Albatross Population on a Remote Island

Catastrophic Risk: Waking Up to the Reality of a Pandemic?

Earthquake-Ridden Area in USA Contains Coccidioides, the Valley Fever Pathogen

Species Identity and Size are Associated with Rat Lungworm Infection in Gastropods

Developing a Global One Health Workforce: The “Rx One Health Summer Institute” Approach

Synergistic Effects of Grassland Fragmentation and Temperature on Bovine Rabies Emergence