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01-08-2019 | Herpes Simplex Type 1

Proteomics analysis of HSV-1-induced alterations in mouse brain microvascular endothelial cells

Authors: Hui Liu, Chu-Xin Huang, Qiang He, Dong Li, Min-Hua Luo, Fei Zhao, Wei Lu

Published in: Journal of NeuroVirology | Issue 4/2019

Abstract

Herpes simplex virus 1 (HSV-1) is a predominant cause of herpes simplex encephalitis (HSE), leading to a high mortality rate and severe neurological sequelae worldwide. HSE is typically accompanied by the blood-brain barrier (BBB) disruption, but the underlying mechanisms are unclear. To explore the disruption mechanisms of the BBB, quantitative analysis of the cellular proteome was carried out to investigate the proteomic changes that occur after infection. In this study, bEnd.3 cells were infected with HSV-1, followed by liquid chromatography-tandem mass spectrometry. A total of 6761 proteins were identified in three independent mass spectrometry analyses. Compared to the uninfected cells, 386 and 293 differentially expressed proteins were markedly upregulated or downregulated, respectively. Bioinformatic analysis showed that the activator protein-1 factor, including Fos, Jun, and ATF family proteins and cell adhesion molecules were significantly changed. Further validation of the changes observed for these proteins was carried out by western blotting and quantitative real-time PCR. Transendothelial electrical resistance (TEER) studies were performed to explore the effects of ATF3, Fra1, or JunB overexpression on the function of bEnd.3 cells. Characterization of the differential expression of these proteins in bEnd.3 cells will facilitate further exploration of BBB disruption upon HSV-1 infection.

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Title: Proteomics analysis of HSV-1-induced alterations in mouse brain microvascular endothelial cells
Authors: Hui Liu
Chu-Xin Huang
Qiang He
Dong Li
Min-Hua Luo
Fei Zhao
Wei Lu
Publication date: 01-08-2019
Publisher: Springer International Publishing
Keywords: Herpes Simplex Type 1
Herpes Simplex Encephalitis
Published in: Journal of NeuroVirology / Issue 4/2019
Print ISSN: 1355-0284
Electronic ISSN: 1538-2443
DOI: https://doi.org/10.1007/s13365-019-00752-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Proteomics analysis of HSV-1-induced alterations in mouse brain microvascular endothelial cells

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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